A kind of preparation method of medicine for parenteral nutrition-related liver disease

A technology of parenteral nutrition and correlation, applied in the field of medicine, to achieve enhanced inhibitory effect, high infection efficiency, and long-term effects

Active Publication Date: 2020-04-28
SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the use of mixed fat emulsions in children with PNALD, so the clinical safety and clinical benefits of mixed fat emulsions in the treatment of PNALD need further investigation

Method used

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  • A kind of preparation method of medicine for parenteral nutrition-related liver disease
  • A kind of preparation method of medicine for parenteral nutrition-related liver disease
  • A kind of preparation method of medicine for parenteral nutrition-related liver disease

Examples

Experimental program
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Effect test

Embodiment 1

[0081] 293T cell culture

[0082] (1) Take out the frozen 293T cells from the refrigerator at -80°C, thaw them immediately in a 37°C water bath, and add them to 1ml of complete medium (DMEM high-glucose medium containing 10% fetal bovine serum) after they are completely thawed , 1000rpm, centrifugal 5min;

[0083] (2) Discard the supernatant, absorb 1 mL of complete medium with a 1 ml gun tip, blow off the cells, transfer to a 10 cm culture dish containing 12 mL of complete medium, and store at 37°C, 5% CO 2 , saturated humidity culture;

[0084] (3) On the second day, discard the culture medium, wash with 1×PBS for 3 times, replace the DMEM medium, and continue culturing. Observe the cell growth and cell status under an inverted phase-contrast microscope every day, and replace the medium every 2 days;

[0085] (4) When the cells are more than 90% confluent, suck off the medium, wash twice with 1×PBS, add 2 mL of 0.25% trypsin, and shake gently to make the trypsin completely...

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Abstract

The invention discloses a preparation method of a medicine applied to parenteral nutrition-associated liver disease. The preparation method comprises the following steps: combining lentivirus packaging plasmid, envelop expression plasmid and interference expression plasmid, so that a three-plasmid lentivirus expression system is obtained; then, promoting co-transfection of the three-plasmid lentivirus expression system into packaging cells; and implementing culture and lysis, so that the parenteral nutrition-associated liver disease drug is obtained. According to the preparation method provided by the invention, shRNA, which has specific interference to an IRE1[alpha] gene, is packaged into lentivirus and is efficiently delivered into BRL cells, so that a stable interference effect on the IRE1[alpha] gene can be achieved, and the parenteral nutrition-associated liver disease can be relieved and treated.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method of medicines for parenteral nutrition-related liver diseases. Background technique [0002] Parenteral nutrition (PN) has brought revolutionary care for newborns who cannot meet their growth through normal intestinal methods. However, there are many related problems in continuous application of PN for a long time (>2 weeks). Parenteral nutrition-associated liver disease (PNALD) is one of the most common complications. With the application of long-term PN, the incidence of PNALD increases. PNALD has fatty liver and jaundice in different degrees. In severe cases, it can progress to liver cirrhosis, and eventually liver failure is life-threatening. Histological evidence of PNAC can be seen after 2 weeks of PN application, and varying degrees of fibrosis can be observed after 6 weeks. However, clinically, liver pathological examination is invas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N5/10C12N15/113A61K31/7105A61K48/00A61P1/16
CPCA61K31/7105C12N15/113C12N15/86C12N2310/141C12N2740/15043
Inventor 朱雪萍汪健朱晓黎崔宁迅崔铭玲李洁陈小倩黄凤霞李之珩
Owner SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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