Preparation method and application of ligustrum lucidum ait total triterpene acid extract

A technology of Ligustrum lucidum and triterpene acid is applied in the field of preparation of a total triterpene acid extract of Ligustrum lucidum, and can solve the problems of unclear chemical components of the extract, low purity of total triterpenoid acids, low overall content, and the like, Achieving the effects of stable and controllable preparation process, good hypolipidemic effect and controllable quality

Active Publication Date: 2017-12-15
SHANDONG PROVINCIAL INST OF DERMATOLOGY & VENEREOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

That is, based on the content of oleanolic acid and ursolic acid, the total triterpene content in the Ligustrum lucidum total triterpene extract prepared by it is only: 25.20%, 23.40%, 22.14% and 25.20%, respectively, the overall content is low , that is to say that the purity of total triterpene acids in the prepared Ligustrum lucidum triterpene extract is too low, and needs to be further improved
[0010] Chinese invention CN105168373 discloses a Ligustrum lucidum extract for lowering blood fat and its preparation method. The Ligustrum lucidum extract prepared by this method has the effect of lowering blood fat, but the medicinal materials used in the extract include Ligustrum lucidum and licorice , the chemical composition of the extract is not clear, which is not conducive to the preparation of modern lipid-lowering traditional Chinese medicine

Method used

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  • Preparation method and application of ligustrum lucidum ait total triterpene acid extract
  • Preparation method and application of ligustrum lucidum ait total triterpene acid extract
  • Preparation method and application of ligustrum lucidum ait total triterpene acid extract

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Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, a kind of Ligustrum lucidum total triterpene acid extract

[0054] A kind of Ligustrum lucidum total triterpene acid extract, its preparation method comprises the steps:

[0055] (1) Pass Ligustrum lucidum powder through a 20-mesh sieve, weigh 5 parts, each 50.0g, use 8 times the amount of 20% ethanol solution containing 1% NaOH to ultrasonically extract once, each time for 0.2h, filter and ultrasonically extract liquid, take the filter residue, neutralize the filter residue with hydrochloric acid, and dry it;

[0056] (2) The dried filter residue obtained in step (1) is extracted once with 8 times the amount of 85% ethanol heat reflux, each time for 1h, the extract is filtered, the filtrate is taken, and the filtrate is neutralized by adding hydrochloric acid and then rotary evaporated to dry, evaporated filtrate with 8 times the amount of 85% ethanol heat reflux extraction once, each time for 1h, filter the extract, take the filtrate, concentrate the fi...

Embodiment 2

[0062] Embodiment 2, a kind of Ligustrum lucidum total triterpene acid extract

[0063] A kind of Ligustrum lucidum total triterpene acid extract, its preparation method comprises the steps:

[0064] (1) Pass Ligustrum lucidum powder through a 20-mesh sieve, weigh 5 parts, each 50.0g, ultrasonically extract 4 times with 15 times the amount of 40% ethanol solution containing 3% NaOH, each time for 2h, filter the ultrasonic extract , take the filter residue, after the filter residue is neutralized with hydrochloric acid, dry;

[0065] (2) The dried filter residue obtained in step (1) is extracted 3 times with 15 times the amount of 96% ethanol under reflux, each time for 2 hours, the extract is filtered, the filtrate is taken, and the filtrate is neutralized by adding hydrochloric acid and then rotary evaporated to dry, evaporated filtrate with 15 times the amount of 96% ethanol heat reflux extraction 3 times, each time 2h, filter the extract, take the filtrate, concentrate the...

Embodiment 3

[0071] Embodiment 3, a kind of Ligustrum lucidum total triterpene acid extract

[0072] A kind of Ligustrum lucidum total triterpene acid extract, its preparation method comprises the steps:

[0073] (1) Pass Ligustrum lucidum powder through a 20-mesh sieve, weigh 5 parts, each 50.0g, use 10 times the amount of 30% ethanol solution containing 2% NaOH to ultrasonically extract twice, each time for 2h, and filter the ultrasonic extract , take the filter residue, after the filter residue is neutralized with hydrochloric acid, dry;

[0074] (2) The dried filter residue obtained in step (1) is extracted 2 times with 10 times the amount of 95% ethanol under reflux, each time for 2 hours, the extract is filtered, the filtrate is taken, and the filtrate is neutralized by adding hydrochloric acid and then rotary evaporated to dry, the filtrate evaporated to dryness was extracted twice with 10 times the amount of 95% ethanol under reflux, each time for 2 hours, the extract was filtered...

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Abstract

The invention discloses a preparation method of a ligustrum lucidum ait total triterpene acid extract. The method comprises the following steps: (1) performing ultrasonic extraction on an ethyl alcohol solution of NaOH; (2) performing heat reflux extraction on ethyl alcohol; (3) performing adsorption by using macroporous resin; (4) performing elution by using a mixed solution of the ethyl alcohol, acetone and water; (5) performing extraction by using petroleum ether and the ethyl alcohol. The prepared ligustrum lucidum ait total triterpene acid extract has high total triterpene acid content (based on oleanolic acid and ursolic acid), namely the purity is high and is up to 70 percent or above; furthermore, the prepared ligustrum lucidum ait total triterpene acid extract contains definite chemical components; a blood fat-reducing medicine prepared from the ligustrum lucidum ait total triterpene acid extract is good in blood fat reducing effect and little in side effect. The preparation method disclosed by the invention is stable, controllable and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of extraction and purification of triterpene acids, and in particular relates to a preparation method of Ligustrum lucidum total triterpene acid extract and its application in the fields of medicine and health care. Background technique [0002] Ligustrum lucidum, another name Ligustrum lucidum Ait. The product is oval, oval or kidney-shaped, with a length of 6-8.5mm and a diameter of 3.5-5.5mm. The surface is black-purple or gray-black, wrinkled and uneven, with scars of fruiting stems or persistent calyx and short stems at the base. Lightweight. The exocarp is thin, the mesocarp is soft and easy to peel off, the endocarp is woody, yellow-brown, with longitudinal ribs, and the seed is usually 1 after breaking, kidney-shaped, purple-black, oily. Odorless, sweet, slightly bitter. [0003] Ligustrum lucidum has a long history of medicinal use. It has been used in my country for more than 2,000 years to treat disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/638A61P3/06A61K36/28A61K131/00
CPCA61K36/28A61K36/638A61K2236/333A61K2236/35A61K2236/39A61K2236/51A61K2236/55A61K2300/00
Inventor 马鲁豫潘晴孙京海
Owner SHANDONG PROVINCIAL INST OF DERMATOLOGY & VENEREOLOGY
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