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Composition using microorganisms to ferment pollen typhae and application thereof

A technology of microbial fermentation and composition, which is applied in the field of medicine, can solve the problems of high heating temperature, destruction of active ingredients of Puhuang, and inability to make full use of active ingredients, so as to achieve high analgesic effect

Inactive Publication Date: 2017-12-15
SANZHU FUER PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing processing technology cannot make full use of its active ingredients, or the active ingredients of Puhuang are destroyed due to excessive heating temperature

Method used

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  • Composition using microorganisms to ferment pollen typhae and application thereof
  • Composition using microorganisms to ferment pollen typhae and application thereof
  • Composition using microorganisms to ferment pollen typhae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of the composition A of fermented Puhuang

[0032]Take 50g of Chinese herb Puhuang, add 1000ml of water to soak for 1.0 hour, add 50g of glucose, 10g of peptone, 1g of yeast extract, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate 7 hydrate, 0.01g of ferrous sulfate 7 hydrate, adjust the pH value to 6.5 . Sterilize at 115°C for 50 minutes, and when the temperature drops to 30°C, inoculate the pre-cultured 5% by volume Monascus (CGMCC 3.567) bacterial solution, and place it on a rotary shaker at 30°C with a speed of 180 r / min Cultivate for 72 hours, then inoculate Bifidobacterium breve (CGMCC 1.2213) and Bifidobacterium adolescentis (CGMCC 1.2190) with a pre-culture volume percentage of 1.0%, culture at 37°C for 6 hours, and then inoculate with a pre-culture volume percentage of 1.0% % of Lactobacillus plantarum (CGMCC 1.19) and Lactobacillus casei (CGMCC1.3206), cultivated at 37°C for 24 hours, then lowered the temperature ...

Embodiment 2

[0033] Embodiment 2: the preparation of the composition B of fermented Puhuang

[0034] Take 200g of Chinese herb Puhuang, add 1000ml of water to soak for 2.0 hours, add 20g of glucose, 5g of peptone, 5g of yeast extract, 2g of dipotassium hydrogen phosphate, 0.2g of magnesium sulfate 7 hydrate, 0.02g of ferrous sulfate 7 hydrate, and adjust the pH value to 6.0 . Sterilize at 115°C for 50 minutes. When the temperature drops to 30°C, inoculate the pre-cultured 6% volume percentage Monascus (CGMCC 3.4701) bacteria solution, and place it on a rotary shaker at 30°C with a speed of 180 r / min Cultivate for 48 hours, then inoculate the pre-cultured 2.0% volume percentage of Bifidobacterium bifidum (CGMCC 1.5090) bacteria solution, cultivate at 37°C for 12 hours, and then inoculate the pre-cultured 2.0% volume percentage of Lactobacillus rhamnosus (CGMCC 1.104) cultured at 37°C for 24 hours, and then lowered the temperature to 28°C for 5 days. Sampling and detection showed that the p...

experiment example 1

[0035] Experimental example 1: Microscopic observation of fermented and unfermented cattail pollen grains

[0036] Get the fermented cattail cattail composition A, B prepared in Example 1, 2 and the non-microbial fermented cattail cattail composition prepared by a similar method, as test samples.

[0037] Take a small amount of the test sample according to the conventional smear method, stain with crystal violet solution and iodine test solution, decolorize with ethanol, and observe under an optical microscope at 400 times magnification.

[0038] Results: Unfermented Puhuang pollen grains have clear edges and complete pollen grains (see attached figure 1 ); the edges of fermented Puhuang pollen grains are blurred, the walls of the pollen grains have been broken, and the contents have leaked out in large quantities (see attached figure 2 , 3 ).

[0039] Conclusion: Microbial fermentation breaks the cell wall of pollen grains of Puhuang, which is beneficial to extract the co...

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Abstract

The invention discloses a composition using microorganisms to ferment pollen typhae and application thereof. The composition using the microorganisms to ferment the pollen typhae is prepared by soaking the typha angustifolia material, preparing, sterilizing, and fermenting by monascus, bifidobacterium and lactobacillus. The composition using the microorganisms to ferment the pollen typhae has the advantages that indicated by observing results under a microscope, the cell wall of the pollen particle of the typha angustifolia is broken, and the content is favorably extracted; the particle of unfermented typha angustifolia pollen is more complete; indicated by component comparison experiment results, the content of polysaccharide is obviously higher than the content of polysaccharide in the unfermented typha angustifolia composition; indicated by pain-relieving action test results, the pain-relieving effect is obviously better than the pain-relieving effect in the unfermented typha angustifolia composition; the fermented typha angustifolia composition can be applied to prevent and treat the blood-stasis pain diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine fermented composition and a traditional Chinese medicine processing technology, in particular to a traditional Chinese medicine Pollen Pollen that is fermented by microorganisms to achieve the purpose of synergizing, and belongs to the technical field of medicine. Background technique [0002] Pollen Typhae is the dried pollen of Typha angustifolia L. orientalis Typha orientalis Presl or the same genus. Puhuang is a classic traditional Chinese medicine. It has been used since the Han Dynasty. It was first recorded in "Shen Nong's Herbal Classic", and it was listed as the top grade. Efficacy: mainly treat amenorrhea, dysmenorrhea, postpartum stagnant abdominal pain, bruises and various bleeding syndromes caused by blood stasis. [0003] In recent years, the research on the chemical composition and pharmacological activity of Puhuang has become more and more in-depth. The research on chemical compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61K36/899A61K45/00A61P29/00A61K35/745A61K35/747
CPCA61K35/745A61K35/747A61K36/062A61K36/88A61K36/899A61K45/00A61K2236/19A61K2300/00
Inventor 吴炳新褚新红孙筱林陆继臣吕美
Owner SANZHU FUER PHARMA
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