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Low temperature storage method of sugarcane pollen

A low-temperature storage and pollen technology, which is applied in botany equipment and methods, plant preservation, application, etc., to achieve the effect of enriching genetic diversity, breaking time and space constraints, and low-temperature storage methods are simple and easy to operate

Active Publication Date: 2020-05-19
广东省科学院南繁种业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a low-temperature storage method for sugarcane pollen, which can solve the problem of providing vigorous male parent pollen materials in sugarcane hybrid breeding when the parental flowering period is not encountered or long-distance hybridization, and has the advantages of flexible storage of sugarcane pollen, reliable technology, and low cost , safe operation, good effect, etc.

Method used

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  • Low temperature storage method of sugarcane pollen
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  • Low temperature storage method of sugarcane pollen

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one, sugarcane pollen low-temperature storage method, comprises the following steps:

[0026] Step 1, sugarcane pollen donor parent processing: the sugarcane female parent to be pollinated is chopped back with stems the day before and put into sulfurous acid stem culture solution to ensure that the sugarcane stems provide sufficient nutrients for flower spikes to bloom.

[0027] Step 2, pollen collection: start observing the opening time of katydid flowers at 7:30 the next morning, collect pollen in time, use a 40-mesh to 50-mesh stainless steel sieve to remove impurities such as anthers, take pure pollen and quickly put it into a dry centrifuge tube for sealed and dark storage .

[0028] Step 3, pollen storage: Weigh the fresh pollen in step 2, fully mix the fresh pollen and color-changing silica gel at a mass ratio of 1:7 to 1:8, put it into a 2ml cryopreservation tube, and let it stand in a refrigerator at 4°C for 8 ~12min, the water content of the pollen...

Embodiment 2

[0032] Embodiment 2, the low-temperature storage method of sugarcane pollen, comprises the following steps:

[0033] Step 1, sugarcane pollen donor parents are treated, the same as in Example 1.

[0034] Step 2, pollen collection, is the same as in Embodiment 1.

[0035] Step 3, pollen storage, weigh the fresh pollen in step 2, mix the fresh pollen and color-changing silica gel particles at a mass ratio of 1:4 to 1:5, put them into a 2ml cryopreservation tube, and place them in a refrigerator at 4°C 15 to 18 minutes to reduce the water content of the pollen to 20% to 15%. Afterwards, the silica gel particles were clamped out with tweezers, sealed and stored at room temperature, 4°C, 0°C, and -20°C, respectively, and the germination rate was measured by pollen germination in vitro at certain intervals.

[0036] The results are shown in Table 2: It can be seen from Table 2 that when the water content of the pollen is reduced to 20%-15%, all the pollen has been inactivated with...

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Abstract

The invention provides a low-temperature storage method of sugarcane pollen. The requirements for different storage time periods of the sugarcane pollen are met in a manner of regulating the moisture content of the fresh sugarcane pollen. The low-temperature storage method comprises the following steps: (I) reducing the moisture content of the fresh sugarcane pollen to 30-25 percent and storing for 1-5 days at a temperature of 0 DEG C; and (II) reducing the moisture content of the fresh sugarcane pollen to 20-15 percent and storing for 5-30 days at a temperature of 20 DEG C below zero. With the adoption of the low-temperature storage method provided by the invention, the vitality time of the sugarcane pollen can be prolonged, the problem of sugarcane parental flowering asynchronism is solved, the time and space limit in sugarcane cross breeding is overcome, the breeding efficiency is improved, and conveniences are provided for germplasm exchange among regions and even countries; and the low-temperature storage method has the advantages of being flexible in sugarcane pollen storage, reliable in technology, low in cost, safe to operate, good in effect and the like.

Description

technical field [0001] The invention relates to a method for low-temperature storage of sugarcane pollen. Background technique [0002] Pollen is indispensable for plants to complete sexual hybridization. It contains all gene types of species and has rich genetic diversity. It is one of the important materials for germplasm storage and exchange. However, under natural conditions, most plant pollen The lifespan of the species is very short, which brings great inconvenience to the work of hybrid breeding, and is also not conducive to related plant genetics research. [0003] In 1922, Knowlton first reported that snapdragon pollen was still viable after storage at -180°C, and found that reducing the water content of pollen could improve the survival rate of snapdragon pollen in frozen storage. In 1938, Brandes and Sartoris used a special container to hold the pollen of a cold-resistant cut-hand strain from the capital of the United States to South America for hybridization wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 常海龙王勤南陈俊吕张伟李奇伟邱永生胡后祥刘壮肖红艳吉家乐吴建涛谢静
Owner 广东省科学院南繁种业研究所
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