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Microsatellite instability site combination, detection kit and application of microsatellite instability site combination

A technology for microsatellite instability and detection reagents, which is used in the determination/inspection of microorganisms, special data processing applications, genomics, etc., can solve the problems of low sensitivity to microsatellite instability, and achieve the effect of improving detection sensitivity

Active Publication Date: 2017-12-26
GENESEEQ TECH INC
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  • Abstract
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Problems solved by technology

[0020] At the same time, the present invention can also solve the problem of low sensitivity in the routine process of determining whether a site is microsatellite instability based on MSI sequencing

Method used

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  • Microsatellite instability site combination, detection kit and application of microsatellite instability site combination
  • Microsatellite instability site combination, detection kit and application of microsatellite instability site combination
  • Microsatellite instability site combination, detection kit and application of microsatellite instability site combination

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Embodiment 1

[0122] The preliminary screening of embodiment 1 locus

[0123] In the following, the present invention demonstrates the application of the analysis process and the MSI site screening method with examples. We performed high-throughput sequencing on the tumor tissue samples of 2,000 Chinese individuals suffering from solid tumors and the corresponding negative control samples of whole blood. The target genomic interval of the detection was the whole exon region of 422 cancer-related genes, and Intronic regions of some common gene fusions. The detection uses the liquid phase capture method to enrich the target DNA. The sequencing process is a common next-generation sequencing method, and its brief description is as follows: extract DNA from tissue samples and whole blood samples of each patient to obtain genomic DNA; Fragmentation to 200-350 base pairs; end repair, addition of adenine, library adapter ligation and other operations on fragmented DNA molecules; obtained DNA libr...

Embodiment 2

[0130] Example 2 Data Analysis of the Unstable State of Microsatellite Sites

[0131] Whole blood samples from 100 healthy people in Example 1 and 163 colorectal cancer clinical samples that had undergone Promega PCR microsatellite detection were used to perform sequencing analysis of microsatellite loci, and the sequencing process was carried out on an illumina next-generation sequencer Carried on.

[0132] First, after processing the off-machine sequencing data of normal population and patient samples, reads that can cover 22 MSI loci are found, and then these reads are normalized according to the number of single base repeats in the microsatellite loci. Classes and numbers accumulate. The main repeat unit types at each locus corresponding to normal samples and patient samples were counted, and the population-dominated repeat unit types on all normal samples were counted. The average value and standard deviation of the main repeat unit types on the 22 MSI sites were counte...

Embodiment 3

[0142] The microsatellite instability analysis of embodiment 3 site combination

[0143] In order to demonstrate the superiority of the 22 microsatellite instability sites combined with biological indicators proposed by the present invention, the 22 microsatellite sites obtained by screening were compared with the 22 randomly selected sites in the remaining sites in the 96 candidate sites. sites, compared in terms of accuracy in defining microsatellite instability in patients.

[0144] Control Experiment 1: Image 6 22 randomly selected microsatellite instability points (numbered in Table 2 are respectively 39, 88, 43, 34, 25, 56, 63, 69, 42, 32, 28, 45, 59, 98, 44 , 50, 85, 99, 67, 53, 52, and 66 MSI sites), only the analysis results obtained by using the determination condition of "the number of main repeating unit types". The classification of MSS, MSI-L, and MSI-H in the figure is based on the judgment results of the Promega kit, and each data point is 163 clinical sam...

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Abstract

The invention provides a microsatellite instability site combination, a detection kit and application of the microsatellite instability site combination. By the used microsatellite instability site combination, after next-generation sequencing (NGS) detection, a sequencing result about one or more microsatellite sites capable of indicating an MSI state can be generated as input data. The method can be used for accurately defining the MSI state is high, low or stable, and has the characteristics of high sensitivity and specificity as well as repeatability in comparison to existing analysis methods.

Description

technical field [0001] The present invention relates to the field of gene detection, in particular, the present invention relates to a method for analyzing detection results of microsatellite instability (MSI) indicator sites based on next-generation sequencing technology (NGS). The method is suitable for simultaneous analysis of one or more microsatellite sites indicating MSI status, accurately assessing the MSI status of patients, and providing theoretical basis and guidance for tumor diagnosis, prognosis, and formulation of clinical treatment plans. Background technique [0002] Microsatellite instability (Micro-Satellite Instability, or MSI) is a molecular phenotype, which is embodied in the presence of insertion or deletion mutations in the repeat sequence of microsatellite loci in the human genome, thereby producing alleles. These insertions or deletions are not corrected by DNA mismatch repair mechanisms (MMR) for a number of reasons. The phenomenon of MSI was first...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11G06F19/18
CPCC12Q1/6886C12Q2600/156C12Q2600/16G16B20/00
Inventor 邵阳马骧原吴雪赵忞超汪笑男张宪那成龙包华欧秋翔
Owner GENESEEQ TECH INC
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