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A kind of coupling method of myoglobin antibody latex microsphere

A myoglobin and microsphere technology, which is applied in the field of latex microsphere antibody preparation, can solve the problems of increased detection-related items, poor protein specificity, prolonged time, etc., and achieves the effects of easy storage, simple operation, and good stability.

Active Publication Date: 2019-02-26
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specificity of this protein is poor, and it can be increased in diseases such as trauma and renal failure, resulting in an increase in detection-related items and a prolonged time

Method used

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  • A kind of coupling method of myoglobin antibody latex microsphere
  • A kind of coupling method of myoglobin antibody latex microsphere
  • A kind of coupling method of myoglobin antibody latex microsphere

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0044] According to the above-mentioned operation procedure, the particle size of the latex microspheres is screened, and the test results are as follows:

[0045]

[0046]The comparison between the actual concentration and the theoretical concentration of the low value, medium value, and high value shows that the difference between the two is small and within ±10%, but the difference between the three different particle sizes is smaller than that of 90nm microspheres.

specific Embodiment 2

[0047] Test the anti-interference ability according to the above-mentioned operation procedures, and the test results are as follows:

[0048]

[0049]

[0050] By comparing the actual concentration with the theoretical concentration of low value, medium value and high value, the recovery test results show that the anti-interference ability of the treated antibody is stronger than that of the untreated antibody.

specific Embodiment 3

[0051] The precision of the antibody latex microspheres coupled to the method according to the above-mentioned operating procedures, the detection results are as follows:

[0052] Intra-assay precision: A certain serum sample was measured 10 times, calculated for the following data, the intra-assay precision difference was 1.18%, and the measured difference was small.

[0053] frequency

1

2

3

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10

Concentration (ng / mL)

161

160

159

163

165

162

159

160

160

160

[0054] Batch-to-batch precision: select 3 different batches of coupled microspheres, and measure a certain serum sample 10 times, the results are shown in the table below.

[0055] 0.67%; compared with the kits of 3 different batches, the difference was small.

[0056]

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Abstract

The invention discloses a coupling method of a myoglobin antibody latex microsphere. A functional solution, which is used during the coupling process, comprises carboxyl latex microspheres, a goat-anti-human myoglobin monoclonal antibody, a coupling agent, an activation liquid, a washing liquid, a confining liquid, and a preserving fluid. The coupling agent comprises carbodiimide and N-hydroxyl succinimide. The myoglobin antibody latex microsphere has the advantages of good stability, high sensitivity, strong anti-interference performance, and easiness for storage. The operation of the coupling method is simple. The myoglobin antibody latex microsphere can be used as a detection reagent of common latex immunoturbidimetry in clinic.

Description

technical field [0001] The invention relates to the technical field of myoglobin content detection, in particular to a method for preparing latex microsphere antibodies in the process of myoglobin content detection. Background technique [0002] Latex particle agglutination test can be used in automatic biochemical analyzers, which produce changes in turbidity by combining with target substances in serum samples, and then reflect changes in absorbance values ​​to generate detection signal values. The detection results are important for disease staging, prognosis and A judgment mark to guide treatment. [0003] At present, the most widely used tests mainly include enzyme-linked immunosorbent assay, immunofiltration colloidal gold staining and radioimmunoassay. Among them, the latex particle agglutination test method has the advantages of fast determination, high sensitivity, good stability of the conjugate, and simple operation. In summary, this method can well make up for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/68G01N33/543G01N33/544
CPCG01N33/531G01N33/54313G01N33/544G01N33/6887G01N2333/805
Inventor 王贤俊何丹
Owner 王贤俊