Preparation method of cell synergistic compound

Abstract

The invention belongs to the technical field of medicine and discloses a preparation method of a cell synergistic compound. The preparation method of the cell synergistic compound comprises the following steps: preparing glycyrrhetinic acid with the concentration of 89.04 [mu]mol / L to 96.8 [mu]mol / L; preparing ophiopogonpolysaccharide with the concentration of 800 [mu]g / mL, atractylodes macrocephalaon polysaccharide with the concentration of 800 [mu]g / mL and saposhnikovia divaricata polysaccharide with the concentration of 800 [mu]g / mL; preparing chebulinic acid with the concentration of 0.04mmol / L to 0.09 mmol / L and tellimagrandin I with the concentration of 0.04 mmol / L to 0.09 mmol / L; mixing and adding into a serum-free culture medium to perform cultivation. The glycyrrhetinic acid hasin vitro proliferation and inhibition effects on a myelogenous leukemia cell line K562, the change of cell cycle caused by the glycyrrhetinic acid is analyzed, and the possible anti-cancer mechanism is discussed; three kinds of traditional Chinese herbal medicine polysaccharide of radix ophiopogonis, bighead atractylodes rhizome and radices sileris are adopted, and the influence of crude ophiopogonpolysaccharide POJ on the proliferation and inhibition effect of leukemia cell K562 cells is detected by an MTT method.

Description

technical field [0001] The invention belongs to the field of medical technology, in particular to a preparation method of a cell synergistic compound. Background technique [0002] Natural killer cells (NK) are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases. Maturation depends on the bone marrow and thymus microenvironment. The function of NK cells is mainly realized in the following ways. NK cell natural killing activity: NK cells recognize target cells (some tumor cells, virus-infected cells, some self-organized cells, parasites, etc.), then NK cells release perforin, cytotoxic factors, and activated NK cells release TNF factors , so as to achieve the possibility of lysing and killing target cells. NK cell cytotoxicity (ADCC effect), mainly due to the FcγRⅢA on the surface of NK cells, which mainly binds to the Fc...

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Problems solved by technology

[0006] To sum up, the problems existing in the prior art are: In order to obtain a larger number of NK cells in the prior art, researchers conduct large-scale reproduction through in vitro culture. However, due to the in vitro environment and the human body The environment is not exactly the same, so the immune activity of NK cells is not very high; the preparation of existing cell synergistic compounds is affected by other factors, the purity of the prepared cell synergistic compounds is not high, and the preparation method is complicated; and for K562 Poor proliferation inhibition in vitro

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