Method for simple and rapid determination of six fatty acid ethyl esters in human whole blood

A technology for rapid determination of fatty acid ethyl esters, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems that plasma or serum biological samples cannot be obtained, no whole blood analysis method has been seen, and the processing steps are cumbersome, etc., to achieve easy The effect of operation and promotion, high detection time requirements, and simple pretreatment process

Active Publication Date: 2018-01-05
FUDAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The currently disclosed methods for the determination of fatty acid ethyl esters in biological samples only detect hair, meconium, and serum, and there are problems such as cumbersome sample pretreatment steps and a large amount of sample materials.
Whole blood is the most important biological sample in fore

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simple and rapid determination of six fatty acid ethyl esters in human whole blood
  • Method for simple and rapid determination of six fatty acid ethyl esters in human whole blood
  • Method for simple and rapid determination of six fatty acid ethyl esters in human whole blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 adopts this method to measure the content of 6 kinds of fatty acid ethyl esters in human whole blood

[0038] Among them, the chromatographic conditions are set as:

[0039] Chromatographic column: HP-5MS elastic quartz capillary gas chromatography column, 30m×0.25mm×0.25μm

[0040] Column temperature: initially 100°C for 1min, then 15°C / min to 210°C and hold for 7min, then 20°C / min to 280°C and hold for 3min

[0041] Solvent delay time: 5min

[0042] Inlet / transfer line temperature: 250°C / 280°C

[0043] Carrier gas: high purity He, constant flow 1mL / min

[0044] Split ratio: 2:1

[0045] EI source electron energy: 70eV

[0046] Scanning method: full scan

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for simple and rapid determination of six fatty acid ethyl esters in human whole blood, belonging to the technical fields of drug detection and forensic identification, wherein the six fatty acid ethyl esters include ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate, ethyl linoleate and ethyl arachidonate. The method is low in the usage amount of samples, and as few as 200 [mu]L of samples are examined. The process of pretreatment is simple and fast. A HP-5MS capillary gas chromatographic column and a mass spectrometric detector are employed for determination of the contents of the six fatty acid ethyl esters in human whole blood. The method has the advantages of high sensitivity, good specificity, a wide linear range, simple and fast operation and low usage amount of extraction solvents, and is capable of satisfying high requirements of urgent forensic identification tasks on detection time and easy to operate and promote.

Description

technical field [0001] The invention belongs to the technical field of drug detection and forensic identification, and particularly relates to a simple and rapid determination of 6 kinds of fatty acid ethyl esters (ethyl myristate, ethyl palmitate, ethyl stearate, ethyl oleate) in human whole blood ester, ethyl linoleate, ethyl arachidonic acid). Background technique [0002] The data shows that the abuse of ethanol is very common in today's society, and has produced many adverse effects on the society. In the work of forensic identification, the detection of ethanol is of great significance for the investigation of the cause of traffic accidents and other cases and the determination of responsibility. However, in actual cases, due to the fast metabolism of ethanol, and sometimes ethanol may be produced in the process of corpse decay, the detection of ethanol in the samples cannot truly reflect the drinking situation. Studies have shown that fatty acid ethyl esters include...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 林泽彬饶渝兰李椒纶张馨予黄志斌沈忆文赵子琴
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap