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A kind of bacterial agent for reducing heavy metal content in vegetables and its application

A technology of heavy metals and bacteria, which is applied in the field of agricultural technology and environmental pollution control, can solve the problems that affect the continuous application of passivation repair agents, hinder the absorption of micronutrient elements, and affect the physical and chemical properties of soil, so as to reduce the content of heavy metals in vegetables and improve the quality of crops. Quality, the effect of promoting vegetable growth

Active Publication Date: 2020-07-31
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Restorative agents or themselves have a certain content of heavy metals (such as phosphate fertilizers, compost, etc.), or will have a significant impact on soil properties and structures (such as lime, clay passivation repair agents), if these passivation repair agents are used in large quantities, it will produce Eutrophication of water body, secondary pollution of heavy metals, hindering the absorption of micronutrient elements, affecting the physical and chemical properties of soil and other adverse effects, and directly affect the continuous application of passivation restoration agents (Wang Liqun et al., 2009; Luo Tao et al., 2007)

Method used

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  • A kind of bacterial agent for reducing heavy metal content in vegetables and its application
  • A kind of bacterial agent for reducing heavy metal content in vegetables and its application
  • A kind of bacterial agent for reducing heavy metal content in vegetables and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The heavy metal-tolerant plant growth-promoting bacteria T1 strain (CCTCC NO: M 2016553) of the present invention was isolated from Cu-contaminated soil in Tangshan, Nanjing, China. The isolation and identification method is as follows: Weigh about 5g of fresh soil sample into a triangular flask containing 45 mL of sterile water, Add several sterile glass beads to the bottle to help release bacteria from the soil particles. After shaking at 180 rpm and 30 minutes, the soil suspension is allowed to stand for 10 minutes, and then applied to a solid medium (beef extract 3.0g, peptone). 5.0g, H 2 O1000ml, agar 20g, pH 7.0-7.2), placed at 30 ℃ for 3 days. Pick a single colony and streak it on the broth solid medium for storage. The strain was inoculated into broth liquid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, pH7.0-7.2), 30℃150rpm shaking culture for 16h, take 1.5mL bacterial solution in Eppendorf centrifuge tube, centrifuge at 5000rpm for 5min to collect the ...

Embodiment 2

[0044] Example 2 Activation of strain T1 and preparation of bacterial suspension

[0045] The T1 (CCTCC NO: M 2016553) slant strain was inoculated into a solid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, agar 20g, pH 7.0-7.2) incubate at 30°C for 3 days. Then select the full and viscous T1 colony to inoculate the liquid medium (beef extract 3.0g, peptone 5.0g, H 2 O 1000ml, pH 7.0-7.2), 30°C and 150rpm shaking culture for 16-20h. The fermentation broth was transferred to a sterile centrifuge bottle, centrifuged at 5000 rpm for 5 minutes to collect the bacteria, washed with sterile deionized water, and resuspended so that the number of cells reached more than 500 million CFU / ml.

Embodiment 3

[0046] Example 3 The ability of strain T1 to produce IAA

[0047] According to the measurement method of Gordon and Weber (1951), aliquot YMA liquid medium in test tubes, 4 mL per tube, and sterilize at 121°C and then add 1 mL of 2.5 mg / mL tryptophan solution sterilized by filtration to make the medium medium color The final concentration of acid is 0.5 mg / mL. The activated T1 bacterial suspension (CCTCC NO: M 2016553) was inoculated into the above medium and cultured in a shaker at 30°C for 3 days. The fermentation broth was centrifuged at 12000r / min for 5min, the supernatant was taken 1mL, 50μL of 10mM orthophosphoric acid was added, and 2mL of Sackowski's color developer was added, mixed thoroughly, developed in the dark at 25°C for 30min, and measured the absorbance at 530nm wavelength. The sterile medium is treated with the same treatment as above as a control and zeroed. The IAA standard solution with the concentration of 0, 5, 10, 20, 40, 60 mg / L was used as a standard c...

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Abstract

The invention discloses a bacterial inoculant for reducing the heavy metal content of vegetables and application thereof. A bacterium T1 for reducing the heavy metal content of the vegetables is classified and named as Serratia sp.T1, and is preserved in China Center for Type Culture Collection (CCTCC), wherein the preservation date is October 10, 2016, and the culture preservation number is CCTCCNO: M2016553. The Serratia sp.T1 strain is taken as a raw material, and is prepared into bacterium suspension after being activated by a culture medium. When the bacterium suspension is inoculated toa lead and cadmium containing liquid culture medium, the pH value of the solution can be increased and the heavy metal can be adsorbed. When the bacterium suspension is inoculated to lead and cadmiumpolluted soil, the exchangeable heavy metal content of the soil can be reduced, the vegetable growth is promoted, the crop quality is improved and the heavy metal content of the vegetables is reduced.

Description

Technical field [0001] The invention belongs to the application field of agricultural technology and environmental pollution treatment, and relates to a bacterial agent for reducing the content of heavy metals in vegetables and its application. Background technique [0002] Vegetable field is a type of agricultural soil with high utilization intensity, high input and output, and large influence by human activities. Many countries have successively experienced the accumulation of heavy metals in vegetable soils and exceeding the standards, which have had a greater impact on vegetable production and threatened human health through the food chain. The development of in-situ passivation methods is currently a better choice for remediation of moderately and lightly polluted soils and heavy metals exceeding the standard. Commonly used materials for stabilization / passivation include inorganic and organic modifiers. There are many varieties. Because of their low cost, they are easy to b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/425C09K101/00
Inventor 盛下放何琳燕韩辉王琪陈玲
Owner NANJING AGRICULTURAL UNIVERSITY