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Microcystins-LR molecular imprinting photo-electrochemical sensor and preparation and application thereof

A microcystin, photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of high cost, inability to achieve online detection, low sensitivity, etc., achieve low cost, simple and fast preparation method , Strong anti-interference ability

Active Publication Date: 2018-01-12
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for detecting microcystin-LR, such as high performance liquid chromatography (HPLC), chromatography / mass spectrometry, Raman detection, etc. Although these methods are sensitive and reliable, they are expensive and require professional technicians. operation, and easy to introduce toxic reagents such as acetonitrile
However, the sensitivity of protease inhibition analysis or physiological toxicity test is low, and timely online detection cannot be achieved.

Method used

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  • Microcystins-LR molecular imprinting photo-electrochemical sensor and preparation and application thereof
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  • Microcystins-LR molecular imprinting photo-electrochemical sensor and preparation and application thereof

Examples

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Effect test

Embodiment 1

[0040] A visible light-driven Cu 2 Preparation of microcystin-LR molecularly imprinted photoelectrochemical sensors of O / PPy composites:

[0041] (1)Cu 2 Preparation of O photoelectric materials: the Cu 2 SO 4 solution with CH 3 CH(OH)COOH solution and a certain amount of PVP according to the molar ratio Cu 2 SO 4 :CH 3 Mix CH(OH)COOH 1:5:0.1 evenly, stir at room temperature for 2 hours, adjust the pH to 8-10 with NaOH solution, use ITO conductive glass as the working electrode, platinum sheet as the counter electrode, and Ag / AgCl as the reference Electrodes were deposited at a constant potential of -0.3V for 150s on a CHI660e workstation at 60°C, and then deposited at a constant potential of -0.2V for 700s. Finally, the electrodeposited Cu 2 The ITO conductive glass of the O photoelectric material is respectively placed in a certain amount of acetone, ethanol, and deionized water for 10 to 20 minutes of ultrasonication, and then placed in a blast drying oven at 40°C to...

Embodiment 2

[0046] Visible light driven Cu 2 Microcystin-LR Molecularly Imprinted Photoelectrochemical Sensor of O / PPy Composite Material Rapid Detection Method of Microcystin-LR:

[0047] (1) Draw a standard curve: use PBS phosphate buffer solution with a pH of 7.2 to 7.4 to configure a series of standard solutions of microcystin-LR, and use the template-removing ITO conductive glass prepared in Example 1 as a work Electrodes, with platinum as the counter electrode, Ag / AgCl as the reference electrode, and visible light as the excitation light source, using i-t technology on the CHI660e electrochemical workstation, adding a bias of -0.2V, in order from low to high concentration Different photocurrents were obtained by detection, and finally a linear relationship was established between the photocurrent density and the concentration of microcystin-LR, and a standard curve was drawn.

[0048] (2) Microcystin-LR of unknown solubility is also configured in PBS phosphate buffer solution with ...

Embodiment 3

[0056] A visible light-driven Cu 2 Preparation of microcystin-LR molecularly imprinted photoelectrochemical sensors of O / PPy composites:

[0057] (1)Cu 2 Preparation of O photoelectric materials: the Cu 2 SO 4 solution with CH 3 CH(OH)COOH solution and a certain amount of PVP according to the molar ratio Cu 2 SO 4 :CH 3 CH(OH)COOH was uniformly mixed at a ratio of 1:5:5, stirred at room temperature for 2 hours, adjusted to pH 8-10 with a certain concentration of NaOH solution, using ITO conductive glass as the working electrode, platinum sheet as the counter electrode, Ag / AgCl As a reference electrode, under the condition of 55 ~ 65 ° C, on the CHI660e workstation, firstly electrodeposited at -0.3V potential constant potential for 100 ~ 200s, then at -0.2V potential constant potential for 600 ~ 800s, and finally electrodeposited Cu 2 The ITO conductive glass of the O photoelectric material is respectively placed in a certain amount of acetone, ethanol, and deionized wa...

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Abstract

The invention relates to a visible light driving Cu2O / PPy composite material based microcystins-LR molecular imprinting photo-electrochemical sensor and preparation and application thereof. The visible light driving Cu2O / PPy composite material based microcystins-LR molecular imprinting photo-electrochemical sensor is characterized in that ITO conducting glass is used as a substrate, and a molecular imprinting film is deposited on the substrate through an electrochemical technology, wherein the molecular imprinting is prepared by compositing a photoelectric material which is a Cu2O semiconductor that can be absorbed within the visible light range and polypyrrole containing microcystins-LR vacancy; the visible light is treated as an excitation light source; the concentration of microcystins-LR is detected by i-t technology through a CHI660e workstation. Compared with the prior art, the microcystins-LR molecular imprinting photo-electrochemical sensor has the advantages of being sensitive to photo-electrochemical response, wide in detection range, capable of detecting within the concentration range of 1.0ng.L<-1> to 10.0 mu g.L<-1>, and high in interference resistance. The preparation method is simple, quick, low in cost, and applicable to quick online detection of water.

Description

technical field [0001] The invention belongs to the field of water pollution analysis and detection, in particular to a visible light-driven Cu 2 O / PPy composite microcystin-LR molecularly imprinted photoelectrochemical sensor and its preparation and application. Background technique [0002] Microcystins (Microcystins) is a monocyclic polypeptide compound released after the death of algae. It has a stable structure, is difficult to degrade, and is easy to accumulate in the body. It is a hepatotoxin with a strong cancer-promoting effect. , protein phosphatase 2A and stomach have a strong inhibitory effect. At present, there are more than 90 kinds of microcystin structures that have been isolated and identified, among which microcystin-LR is the most toxic and most abundant subtype. The World Health Organization (WHO) has also stipulated that the maximum content of microcystins in drinking water should not exceed 1ug·L -1 . In view of this, it is necessary to develop micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/333G01N27/36G01N27/48
Inventor 贾能勤高攀陈俊利张雨王海李鹏威
Owner SHANGHAI NORMAL UNIVERSITY
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