Preparation method of fusiform nanomicelles

A technology of nano-micelle and nano-composite glue is applied in the field of preparation of shuttle-shaped nano-micelle, which can solve the problems of complex assembly rules and no self-assembly.

Active Publication Date: 2018-01-19
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Proteins are high molecular polymer materials and also block copolymers. Proteins with a certain structure can spontaneously assemble into assemblies with certain shapes in solution. However, no one has studied the assembly of proteins so far. The assembly process is not only Affected by

Method used

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  • Preparation method of fusiform nanomicelles
  • Preparation method of fusiform nanomicelles

Examples

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Embodiment 1

[0018] A preparation method of spindle-shaped nano micelles, comprising the following steps:

[0019] 1. Use sodium carbonate solution to prepare sericin: weigh a certain amount of silkworm cocoons, under the conditions of temperature 95°C, pH 13, mass concentration of sodium carbonate at about 8g / L, bath ratio 50:1, and reaction time 2 hours. Silk is degummed, and deionized water is used to prepare a sodium carbonate degumming solution.

[0020] 2. Purification of sericin: filter the sericin solution prepared in the above steps with roving and qualitative filter paper in order to remove insoluble impurities and undissolved proteins; then use a dialysis bag with a molecular weight cut-off of 500Da for dialysis, To remove sericin segments with too small molecular weight and excess Ca in the solution 2+ 、Na + , CO 3 2+ ions and other miscellaneous ions; centrifuge the obtained sericin solution in a high-speed centrifuge with a rotation speed of 12000 rpm to obtain sericin. ...

Embodiment 2

[0025] A preparation method of spindle-shaped nano micelles, comprising the following steps:

[0026] 1. Use sodium carbonate solution to prepare sericin: weigh a certain amount of silkworm cocoons, under the conditions of temperature 90°C, pH 12, mass concentration of sodium carbonate at 5g / L, bath ratio 50:1, and reaction time 3 hours. For degumming, a sodium carbonate degumming solution was prepared using deionized water.

[0027] 2. Purification of sericin: filter the sericin solution prepared in the above steps with roving and qualitative filter paper in order to remove insoluble impurities and undissolved proteins; then use a dialysis bag with a molecular weight cut-off of 500Da for dialysis, To remove sericin segments with too small molecular weight and excess Ca in the solution 2+ 、Na + , CO 3 2+ ions and other miscellaneous ions; centrifuge the obtained sericin solution in a high-speed centrifuge with a rotation speed of 12000 rpm to obtain sericin with a weight a...

Embodiment 3

[0031] A preparation method of spindle-shaped nano micelles, comprising the following steps:

[0032]1. Use sodium carbonate solution to prepare sericin: weigh a certain amount of silkworm cocoons under the conditions of temperature 90°C, pH 12.5, sodium carbonate concentration 6g / L, bath ratio 50:1, and reaction time 3 hours Degumming the silk, using deionized water to prepare a sodium carbonate degumming solution.

[0033] 2. Purification of sericin: filter the sericin solution prepared in the above steps with roving and qualitative filter paper in order to remove insoluble impurities and undissolved proteins; then use a dialysis bag with a molecular weight cut-off of 500Da for dialysis, To remove sericin chain segments with too small molecular weight and excessive Ca2+, Na+, CO32+ ions and other miscellaneous ions in the solution; centrifuge the obtained sericin solution in a high-speed centrifuge with a rotation speed of 12000 rpm to obtain For sericin with a molecular we...

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Abstract

The invention discloses a preparation method of fusiform nanomicelles, and solves the technical problems that at present, no self-assemblies with various morphologic characteristics and different properties are assembled from different proteins. The preparation method comprises the following steps: 1, preparing a sericin solution and an acid-process glutin liquid: adding sericin into deionized water to obtain the sericin solution with the mass concentration of 0.5-1.5%, and preparing the acid-process glutin solution with the concentration of 0.5-1.5% by using deionized water; 2, mixing the sericin solution and the acid-process glutin solution which are prepared in the step 1 to obtain a mixed solution, and adjusting the pH value of the mixed solution to be between isoelectric points of thetwo proteins; 3, assembling the fusiform nanocomposite micelles: uniformly stirring the mixed solution prepared in the step 2, and assembling at constant temperature for 12-18 hours to obtain the fusiform nanocomposite micelles. The composite micelles have good electrochemical stability, dilution stability and storage stability.

Description

technical field [0001] The invention relates to the field of polymer nanomaterials, in particular to a method for preparing a shuttle-shaped nanomicelle by using sericin and acid-processed gelatin. Background technique [0002] Polymer self-assembly refers to the process in which macromolecules spontaneously form a thermodynamically stable ordered molecular structure driven by weak interaction forces such as hydrogen bonds, electrostatic interactions, hydrophobic interactions, and van der Waals forces. In recent years, self-assembly technology has attracted extensive attention in the scientific field and has a good application prospect. Related research is of great significance for the design of polymer aggregates and new materials with highly ordered structures and controllable functions. [0003] The preparation methods of polymer nanomaterials mainly include covalent bond micelle assembly, heterogeneous polymerization, template method, non-covalent bond micelle self-asse...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08K5/07B82Y30/00B82Y40/00
Inventor 苏小舟栗蕾
Owner ZHONGYUAN ENGINEERING COLLEGE
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