Tissue culture and propagation method of clerodendrom bungei

A technique for tissue culture, stink peony, applied in the field of plant tissue culture

Inactive Publication Date: 2018-02-02
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Tissue culture and propagation method of clerodendrom bungei
  • Tissue culture and propagation method of clerodendrom bungei
  • Tissue culture and propagation method of clerodendrom bungei

Examples

Experimental program
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Effect test

Embodiment 1

[0041] (1) Acquisition of aseptic materials and induction culture: cut the young axillary buds of peony stinkbug with good growth and no pests and diseases, wash them with tap water for 30 minutes, then shake them in an aqueous solution with a volume concentration of 1% detergent for 2 minutes, and use sterile Rinse with sterile water; soak 30s in 70% volume concentration ethanol water solution on the ultra-clean workbench, then soak in mixed disinfectant solution for 10min, and then rinse with sterile water for 3 times; blot dry water on sterilized filter paper, and dissect Peel off the 0.3mm axillary bud tip with a knife, then inoculate the axillary bud tip into a culture bottle filled with cluster bud induction medium, cover the bottle cap and seal the bottle mouth with a parafilm, and place it in an incubator at 24°C with a light of 1500lx Cultivate under conditions for 15 days; the mixed disinfectant is a mixture of Tween 20 and 84 disinfectant with a volume ratio of 1:200...

Embodiment 2

[0045] The influence of different disinfectants of embodiment 2 on the growth of stink peony explants

[0046] 10% hydrogen peroxide (H 2 o 2 ) aqueous solution, mercuric chloride (HgCl) with a mass concentration of 0.1% 2 ) aqueous solution and the mixed disinfectant solution in embodiment 1 to sterilize the explant material (i.e. axillary bud) of stink peony, and other operations are the same as in embodiment 1. The axillary buds were inoculated into culture bottles filled with cluster bud induction medium, and the bottles were sealed with a parafilm and placed in an incubator. After cultivating at 24° C. under the condition of 1500 lx light for 15 days, observe and count the effects of different disinfectants on the induction and growth of stink peony buds. The results are shown in Table 1. The results showed that the disinfection effect of hydrogen peroxide with a mass concentration of 10% was slightly worse, the pollution rate reached 20%, and the growth was slow. But...

Embodiment 3

[0049] Example 3 The Induction Effect of Different Hormone Combinations on Smelly Peony Buds

[0050] The axillary buds of stinky peony were respectively inoculated in the MS basic medium (namely the cluster bud induction medium) added with different concentrations of 6-BA and NAA, and other operations were the same as in Example 1. The results showed that the medium with different combinations of hormones could induce the formation of shoots to varying degrees. Among them, the medium MS+6-BA2.0mg / L+NAA0.2mg / L+0.4g / L acid hydrolyzed casein is the best, the most regenerated buds are induced, the growth is fast, the average bud length is 1.55cm, and the bud induction The seedling rate was 90%, the seedlings were large and strong, and there was no vitrified seedling phenomenon, and the incision parts of the medium without adding acid hydrolyzed casein all showed different degrees of browning.

[0051] Bud induction rate = number of bud induction / number of explants × 100%

[0...

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Abstract

The invention discloses a tissue culture and propagation method of clerodendrom bungei. A large number of high-quality clerodendrom bungei seedlings can be obtained in a short period, and the market requirements are met. The method comprises the steps of aseptic material obtaining, bud induction culture, bud propagation, rooting and rooting seedling transplanting. Axillary buds are used as explants; fast propagation is performed by a tissue culture method; the culture medium is improved; the trophic factors such as acid hydrolyzed casein are added; the defects of long conventional cottage anddivision propagation period, low propagation coefficient, easy infection, virus spreading and the like of the clerodendrom bungei are overcome. The tissue culture seedlings produced by the method havethe advantages of few viruses, inheritance stability and the like.

Description

(1) Technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid seedling propagation of stink peony. By this method, large-scale production of fine seedlings can be carried out. (2) Background technology [0002] Clerodendrom bungei Steud is a dicotyledonous plant belonging to the genus Clerodendrom bungei. Stinky peony is widely distributed in my country, mainly in Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan, Hubei, Guangxi and other places. Its leaves are dark green, its flowers are beautiful, and its flowering period is long. It is a very beautiful garden flower. In addition to ornamental value, it also has important medical and medicinal value. According to historical records, stinky peony has the effects of promoting blood circulation and dispelling blood stasis, reducing swelling and pain, clearing heat and dampness. "Zhejiang Folk Herbal Medicine" records: Smelly Peony "clears hea...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 邹克琴李素芳管峰
Owner CHINA JILIANG UNIV
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