Separation method of total ginsenosides

A technology for the separation of total ginseng saponins and its separation method, which is applied in the field of preparation of natural pharmaceutical ingredients, and achieves the effects of high separation efficiency, simple device, and reduced solvent consumption

Active Publication Date: 2020-10-02
JILIN YATAI PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the field of traditional Chinese medicine analysis, carbon nanotubes are mainly used to enrich the pesticide residues in traditional Chinese medicines, and their application in the extraction and separation of active ingredients in traditional Chinese medicines is still seldom, but there is no report on the separation of total ginseng saponins

Method used

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  • Separation method of total ginsenosides
  • Separation method of total ginsenosides
  • Separation method of total ginsenosides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) extract ginseng extract

[0076] Take fresh ginseng roots, wash them, and place them in a blast drying oven with a temperature controlled at 35°C to dry to constant weight. Dried ginseng is pulverized with a pulverizer, and 40 mesh sieves are used to obtain ginseng powder.

[0077] Get 5 g of ginseng powder and add it to 15 ml of 80% (volume fraction) ethanol, and extract it ultrasonically at room temperature and pressure for 15 minutes. Centrifuge at 4000rpm for 5 minutes to separate the ginseng powder. The separated ginseng powder was extracted twice again under the aforementioned conditions, the extraction solvents were combined, and the extract was diluted 10 times with 80% ethanol to obtain the diluted ginseng extract.

[0078] (2) Adsorption

[0079] Take 0.3 g of multi-walled carbon nanotubes and place them in 50 ml of diluted ginseng extract, and vortex for 10 seconds to fully contact the multi-walled carbon nanotubes with the diluted ginseng extract. Th...

Embodiment 2

[0085] The experimental method is the same as that in Example 1, except that the difference from Example 1 is that the extraction solvent used to extract the ginseng extract is methanol, and it is centrifuged at 4000 rpm for 10 minutes. The desorption efficiency of ginsenoside is 88.2-94.8%.

Embodiment 3

[0087] The experimental method is the same as that of Example 1, except that the extraction solvent of the ginseng extract is 95% ethanol, which is centrifuged at 8000 rpm for 4 minutes. The desorption efficiency of ginsenoside is 85.1-95.4%.

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Abstract

The invention provides a method for separating total saponins of ginseng. The method comprises the following steps: adding ginseng powder into an extraction solvent, carrying out ultrasonic extractionunder the conditions of room temperature and normal pressure, then carrying out centrifugal separation, collecting liquid, and diluting the collected liquid with the extraction solvent so as to obtain a ginseng extracting solution; adding multi-walled carbon nanotubes into the ginseng extracting solution for adsorbing the total saponins, then carrying out centrifugal separation, and collecting solid; adding the solid into an elution solvent for eluting, then carrying out centrifugal separation, and collecting eluant to obtain the total saponins of the ginseng. The method provided by the invention has the advantages of high separation efficiency, short time consumption, less solvent consumption, simple operation and simple equipment.

Description

technical field [0001] The invention belongs to the technical field of preparation of natural medicinal components. Specifically, the invention relates to a method for separating total ginsenosides. More specifically, the invention relates to a method for extracting and rapidly separating total ginsenosides from ginseng. Background technique [0002] Ginseng (Panax ginseng C.A.Mayer) is a perennial Araliaceae plant of the genus Panax, mainly produced in East Asia, and has a medicinal basis in China for thousands of years. The "Shen Nong's Materia Medica" in the Qin and Han Dynasties listed it as top grade. Ginsenosides are a class of glycoside compounds composed of saponin and sugar substituents, and are the main active ingredients of ginseng to exert pharmacological effects such as anti-aging, anti-tumor, lowering blood sugar, improving endocrine and regulating the immune system. Ginsenosides in ginseng are divided into protopanaxadiol type, protopanaxatriol type and olean...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258
CPCA61K36/258A61K2236/53A61K2236/55
Inventor 修洋李镇文赵幻希苗瑞刘淑莹孙秀丽
Owner JILIN YATAI PHARM CO LTD
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