Compound vaccine for specifically preventing and treating escherichia coli-induced diarrhea of donkey foals

A technology of Escherichia coli and Escherichia coli, applied in the field of biomedicine, can solve problems such as antibiotic food safety, microcirculation failure, donkey foal death, etc., to slow down the production of animal-derived drug-resistant bacteria and stimulate mucosal immunity Response, enhance the effect of immunogenicity

Active Publication Date: 2018-02-16
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the underdevelopment of various systems of the donkey foal and poor resistance, the course of the disease occurs rapidly, and dehydration, microcirculation failure and shock often occur without treatment in time, resulting in the rapid death of the donkey foal.
In veterinary clinics, the abuse of antibiotics has also created serious food safety problems

Method used

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  • Compound vaccine for specifically preventing and treating escherichia coli-induced diarrhea of donkey foals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, isolation culture and identification of donkey diarrhea Escherichia coli La strain

[0060] 1. Isolation and cultivation of donkey diarrheal Escherichia coli La strain

[0061] Anal swabs of donkey foals with diarrhea were aseptically collected, spread on nutrient agar plates, and incubated in a 37°C incubator for 24 hours. Pick round convex, smooth, translucent, 2-3 mm in diameter, off-white colonies characteristic of Escherichia coli from the culture medium, smear them on nutrient agar plates, culture them in a 37°C incubator for 24 hours, and pass them for 3 generations Separation and purification were carried out to obtain donkey diarrhea Escherichia coli La strain.

[0062] 2. Identification of donkey diarrheal Escherichia coli La strain

[0063] 1. Physiological and biochemical identification

[0064] Physiological and biochemical identification was identified by XK automatic bacterial identification / drug sensitivity analyzer. Specific steps are ...

Embodiment 2

[0100] Embodiment 2, the preparation of the compound vaccine of specific prevention and treatment donkey foal Escherichia coli diarrhea

[0101] 1. Preparation of formaldehyde-inactivated bacterin

[0102] 1. Mix the nutrient broth culture solution of Escherichia coli La with donkey diarrhea and aqueous formaldehyde solution to obtain an inactivation system. The concentration of E. The volume fraction in the living system is 0.5%. Donkey diarrhea Escherichia coli La strain nutrient broth culture fluid is that the donkey diarrhea Escherichia coli La strain isolated in embodiment 1 is inoculated in nutrient broth culture fluid (nutrient broth culture fluid is peptone 10.0g, ground beef 3.0g Dissolve 5.0 g of sodium chloride in 1 L of distilled water) and mix them evenly.

[0103] Place the inactivation system in a 37°C incubator for 48-72 hours to kill the bacteria and obtain the inactivated bacteria liquid.

[0104] 2, add sodium thiosulfate aqueous solution (concentration o...

Embodiment 3

[0117] 实施例3、特异性防治驴驹大肠杆菌性腹泻的复合疫苗的应用

[0118] 一、致病性检测试验

[0119] 1、菌液制备

[0120] 将致病性大肠杆菌La株接种于LB培养基中,37℃摇菌24h;取20μL菌液倍比稀释后,涂板培养计数,计算结果表明:每毫升菌液中细菌含量为5亿。

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Abstract

The invention discloses a compound vaccine for specifically preventing and treating escherichia coli-induced diarrhea of donkey foals. By preparing a specific escherichia coli strain, separated from adonkey body, and fimbriae subunits into a thallus-fimbriae subunit compound vaccine, the invention provides the compound vaccine for specifically preventing and treating the escherichia coli-induceddiarrhea of the donkey foals and establishes a specific micro-ecological prevention and control system for comprehensively preventing and treating the escherichia coli-induced diarrhea of the donkey foals. The compound vaccine provided by the invention can effectively improve the immunogenicity of antigen, stimulate mucosal immune response and enhance the prevention effect on the escherichia coli-induced diarrhea, and has a function of preventing and controlling the escherichia coli-induced diarrhea of the donkey foals. Compared with the prior art, not only can the compound vaccine reduce theuse of antibacterial drugs for animals, reduce antibacterial drug residues in animal products and slow down the generation of animal-derived drug-resistant bacteria, but also avoids the spread of thedrug-resistant bacteria between animals or between animals and people. The compound vaccine is expected to become an excellent specific vaccine for clinical prevention of escherichia coli.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a compound vaccine for specifically preventing and treating Escherichia coli diarrhea in donkey foals. Background technique [0002] The incidence of diarrhea in newborn donkey foals in large-scale donkey farms is between 30% and 50%, and the peak age is 15-45 days. Most cases of diarrhea in donkey foals are not effective after antibiotic treatment, and about 15% of the cases It died within 48h-72h of onset, which brought huge economic losses to large-scale farms. There are many reasons for donkey diarrhea, but the main pathogenic factor is pathogenic Escherichia coli. At present, the disease is usually treated with antibiotic drugs, combined with supportive therapy. However, due to the underdevelopment of various systems of the donkey foal, poor resistance and rapid course of the disease, dehydration, microcirculatory failure and shock often occur before treatment, resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/108A61P31/04A61P1/12
CPCA61K39/0258A61K2039/521A61K2039/552Y02A50/30
Inventor 刘文强王仁虎姜桂苗高维平秦绪岭刘桂芹夏楠
Owner LIAOCHENG UNIV
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