Serum for treating otitis media caused by Haemophilus influenzae and application of the serum

A technology of Haemophilus influenzae and otitis media, applied in the direction of serum immunoglobulin, sensory disease, anti-bacterial immunoglobulin, etc., can solve the problems of bacteria dispersion, insufficient acquisition, transmission, etc., and achieve the effect of easy absorption

Active Publication Date: 2020-07-14
ZHEJIANG MEDICAL COLLEGE
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  • Abstract
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Problems solved by technology

However, studies have found that during the growth of Haemophilus influenzae, when the expression of Hap reaches a certain level, it will initiate self-dissociation, which will eventually lead to the dispersion of bacteria and spread in the respiratory tract. Therefore, ordinary bacterial culture is not enough to obtain enough Hap Protein, to obtain a large amount of Hap protein as an antigen to prepare antibodies requires biological modification

Method used

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  • Serum for treating otitis media caused by Haemophilus influenzae and application of the serum
  • Serum for treating otitis media caused by Haemophilus influenzae and application of the serum
  • Serum for treating otitis media caused by Haemophilus influenzae and application of the serum

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Experimental program
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Embodiment

[0018] Materials and Methods

[0019] Sources of strains, sera and primary reagents

[0020] Non-typeable Haemophilus influenzae standard strain ATCC49247 (NTHi), encapsulated Haemophilus influenzae standard strain ATCC9327 (Hia), ATCC 9334 (Hib), ATCC 9007 (Hic), ATCC 9332 (Hid), ATCC 8142 (Hie) and ATCC 9833 (Hif) provided by the Department of Pathogen Biology, School of Medicine, Zhejiang University. Clean-grade Qingzilan rabbits, 3-4 weeks old, clean-grade BALB / c mice, female, 6-8 weeks old, weighing (18±1) g, were provided by the Animal Experiment Center of Hangzhou Medical College. This study complied with the ethical standards established by the Experimental Animal Ethics Committee of Hangzhou Medical College. Each Hi strain was subcultured on Columbia blood agar plate (bioMérieux) at 37ºC.

[0021] Construction and Cloning of Antigen Epitope Fusion Fragment

[0022] Genomic DNA of ATCC49247 strain was extracted by Bacterial Genomic DNA Extraction Kit (Axygen), and ...

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Abstract

The invention discloses serum for treating otitis media caused by haemophilus influenza and an application thereof and belongs to the technical field of biology. The serum is obtained by immunizationof recombinant protein MAP-rHap-C4GGGS-Hap-C150. The serum (serum diluent) has an effective-sterilization function. In addition to antibody ingredients with a sterilization function, the other ingredients in the serum are similar to the serum ingredients of a human body, and if being prepared into ear drops by aseptic and biosafety treatment to be used for treating the otitis media, the serum hasthe advantages of easiness in absorption, no use of hormone, no generation of dependency and suitability for use of infants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a serum for treating otitis media caused by Haemophilus influenzae and an application of the serum. Background technique [0002] Haemophilus influenzae ( Haemophilus influenzae , Hi) are Gram-negative small bacilli that parasitize in the nasopharynx and upper respiratory tract of humans. The respiratory tract is the main entry portal and can cause infection in multiple parts of the body. In recent years, studies have reported that the detection rate of Hi in acute suppurative otitis media (especially children under the age of 12) has increased significantly. Haemophilus influenzae can be divided into capsular type and non-capsular type. The former can be divided into 6 serotypes by capsular antibody, so capsular type Hi is also called typeable Haemophilus influenzae (typeable H. influenzae , THi); the latter cannot be typed with capsular polysaccharide antibody, so no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/06C07K16/12C12N15/70A61K39/40A61P27/16
CPCA61K2039/505C07K16/06C07K16/1242C12N15/70
Inventor 孙爱华严杰范兴丽张新蔚
Owner ZHEJIANG MEDICAL COLLEGE
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