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A kind of inoculation method of oyster mushroom culture medium

A culture medium and abalone mushroom technology, applied in mushroom cultivation, botanical equipment and methods, cultivation, etc., can solve the problems of large gap between bacteria strain and mushroom bag culture medium, irregular fruiting, low inoculation efficiency, etc., and achieve reduction Possibility of contamination, equalization of planting depth, and effect of reducing the probability of uneven fruiting

Active Publication Date: 2020-10-16
黎芷杉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The probability of contamination is high if the holes are drilled before inoculating the bacteria; the gap between the bacteria and the bacteria bag medium is large, the fruiting is irregular and the inoculation efficiency is low

Method used

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  • A kind of inoculation method of oyster mushroom culture medium
  • A kind of inoculation method of oyster mushroom culture medium
  • A kind of inoculation method of oyster mushroom culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The inoculation of the oyster mushroom culture medium can be completed by adopting the following process steps:

[0047] (1) Put the culture material for cultivating abalone mushroom into a plastic bag, tie the mouth of the bag tightly, obtain the culture medium after sterilization, and set aside;

[0048] (2) Put multiple bamboo or wooden perforated inoculation sticks with multiple strain slots in the disinfection pot, boil the water in the pot for disinfection for 15-30 minutes, take it out and cool it naturally for later use;

[0049] (3) Insert the sterilized perforated inoculation rod into the strain of oyster mushroom, so that the strain of oyster mushroom is attached to the strain groove of the perforated inoculation rod;

[0050] (4) Insert the perforated inoculation stick with abalone mushroom strains into the medium in a linear shape from the surface of the medium. After insertion, the perforated inoculation stick cannot be taken out and left in the medium; ev...

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PUM

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Abstract

The invention discloses an inoculation method of a pleurotus abalonus culture medium. The method comprises the following inoculation steps of: (1) putting a culture material for cultivating pleurotusabalonus into a plastic bag, tightening a bag opening, and carrying out sterilization to obtain a culture medium for later use; (2) sterilizing a plurality of perforated inoculation rods made of bamboo or wood and provided with a plurality of strain grooves for later use; (3) inserting the sterilized perforated inoculation rods into pleurotus abalonus strains to enable the pleurotus abalonus strains to be attached to the strain grooves of the perforated inoculation rods; (4) completely inserting the perforated inoculation rods attached with the pleurotus abalonus strains into the culture medium in a linear shape from the surface of the culture medium without taking out the perforated inoculation rods after insertion but leaving the perforated inoculation rods in the culture medium; and (5)stacking the culture medium with the inserted perforated inoculation rods on a culture frame, and guaranteeing the side, with the inserted perforated inoculation rods, of the culture medium to be upward, so as to complete inoculation. The raw materials of the culture material comprise mulberry branches, bamboo branches, peanut shells, siraitia grosvenorii vines, corn cob residues, bagasse, limestone powder and waste molasses. The method has a high inoculation efficiency and can reduce the probability of contamination of strain inoculation.

Description

technical field [0001] The invention relates to the technical field of abalone mushroom cultivation, in particular to an inoculation method of abalone mushroom culture medium. Background technique [0002] Abalone mushroom, Latin scientific name: Pleurotus abalonus , alias China Taiwan Pleurotus ostreatus, the mushroom is rich in nutrients, the flesh is thick, and the flavor is unique. Every 100 grams of dry product contains 7.8 grams of protein, 2.3 grams of fat, 10.2 grams of water, 69 grams of polysaccharides, 5.6 grams of crude fiber, and 21 mg of calcium. , 220 mg of phosphorus, 3.2 mg of iron, 0.12 mg of vitamin B1, 7.09 mg of B2, 6.7 mg of niacin, and 8 kinds of essential amino acids. Among the non-essential amino acids, glutamic acid, aspartic amino acid, and alanine are the most, and are deeply loved by the local people. Abalone mushroom is slightly warm in nature and sweet in taste. It has the effects of nourishing, tonifying the spleen and stomach, eliminating w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/30A01G18/00
CPCC05D3/02C05F17/00C05G3/00C05F5/002C05F5/006C05F11/00C05F11/08
Inventor 黄兴根
Owner 黎芷杉
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