Forming system and bio-3D printing forming method for preparing tissue-engineered osteochondral scaffold
A tissue engineering bone, 3D printing technology, applied in tissue regeneration, additive processing, prosthesis, etc., can solve the problems of easy formation of fibrocartilage, tissue damage, inability to regenerate osteochondral tissue, etc., to achieve simple and fast switching and improve performance , the effect of a good transition effect
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Embodiment 1
[0036] In this example, see Figure 1~3 , a forming system for preparing tissue-engineered osteochondral scaffolds, mainly composed of a three-dimensional motion mechanism 1, a nozzle device 2, a pressure source 3, a control and data processing system 4, and a forming platform 5; the nozzle device 2 mainly includes a barrel 8 and parallel double-needle 9; using pneumatic extrusion molding process device, the pressure source 3 communicates with the barrel 8 through the air pipe, and the barrel 8 communicates with the parallel double-needle 9 through the hose to form the extrusion pipeline for the slurry The control and data processing system 4 is respectively connected to the three-dimensional motion mechanism 1 and the pressure source 3 through the data line, the three-dimensional motion mechanism 1 pulls the forming table 5 to carry out three-dimensional movement in space, coordinates the movement of the forming table 5 and starts and stops the feeding, and the three-dimension...
Embodiment 2
[0048] This embodiment is basically the same as Embodiment 1, especially in that:
[0049] In this embodiment, a biological 3D printing forming method includes the following steps:
[0050] a. Prepare an aqueous gelatin solution with a mass percentage of 12%, add a sodium alginate solution with a mass percentage of 4% to the gelatin aqueous solution, and mix evenly according to the volume ratio of the gelatin aqueous solution and sodium alginate solution at a ratio of 1:1, and prepare Forming material hydrogel B, obtain gelatin-sodium alginate mixed solution 10ml, as cartilage layer material; Prepare the gelatin aqueous solution that mass percent is 20%, add the chitosan solution that mass percent is 5% to gelatin aqueous solution, according to gelatin The volume ratio of the aqueous solution and the chitosan solution is 1:2 and mixed evenly, and hydroxyapatite is added to enhance the mechanical strength of the prepared scaffold, and the forming material hydrogel A is prepared...
Embodiment 3
[0058] This embodiment is basically the same as the previous embodiment, and the special features are:
[0059] In this embodiment, a biological 3D printing forming method includes the following steps:
[0060] a. preparing an aqueous gelatin solution with a mass percentage of 15%, adding mTG enzyme pre-crosslinking to the gelatin aqueous solution, the mixing mass ratio of gelatin and mTG enzyme is 30:1, preparing the forming material hydrogel B according to the method, and obtaining gelatin precrosslinking Combined solution 10ml, as cartilage layer material;
[0061] Prepare an aqueous gelatin solution with a mass percentage of 20%, add a chitosan solution with a mass percentage of 5% to the gelatin aqueous solution, mix evenly according to the volume ratio of the gelatin aqueous solution and chitosan solution at a ratio of 1:2, and add hydroxyl Apatite is used to enhance the mechanical strength of the prepared bracket, and the forming material hydrogel A is prepared to obta...
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