A kind of biological bacterial fertilizer, its preparation method and its application in soil phosphorus decomposing

A bio-bacterial fertilizer and soil technology, applied in biochemical equipment and methods, microorganism-based methods, fertilization methods, etc., can solve the problem of low nitrogen fertilizer improvement efficiency, achieve good growth promotion effect, high fertilizer efficiency, and improve phosphorus removal effect. Effect

Active Publication Date: 2021-06-04
内蒙古力能肥业生物科技有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the single use of phosphorus solubilizing bacteria in the soil is not efficient in increasing nitrogen fertilizer in the soil, and the synergistic effect of EM bacteria liquid and phosphorus solubilizing bacteria is used to improve the content of phosphorus and nitrogen fertilizer in the soil. ; Simultaneously the present invention also provides a kind of carrier of this compound bacterial fertilizer

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  • A kind of biological bacterial fertilizer, its preparation method and its application in soil phosphorus decomposing
  • A kind of biological bacterial fertilizer, its preparation method and its application in soil phosphorus decomposing
  • A kind of biological bacterial fertilizer, its preparation method and its application in soil phosphorus decomposing

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preparation example Construction

[0032]The present invention also provides the preparation method of the above-mentioned biological bacterial fertilizer. The above-mentioned preparation method of the biological bacterial fertilizer comprises the following steps: Step 1, after activating the phosphate-solubilizing bacteria, inoculate it on the activated medium and cultivate it for 12-36 hours; The second step is to select the strains on the activated medium, inoculate them in the fermentation medium, then add the EM stock solution to carry out fermentation, the temperature is 30-35°C, and the fermentation time is 12-36h. After fermentation, the fermentation liquid is obtained; the third step , the biological fertilizer obtained after mixing the fermentation broth with the adsorbent carrier. Described activation culture medium is calculated according to 1 liter composition ratio and is: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0~8.0; fermentation mediu...

Embodiment 1

[0034] Embodiment 1 Preparation of compound biological bacterial fertilizer

[0035] Preparation of fermentation broth:

[0036] Activate the phosphate-solubilizing bacterium Bacillus subtilis HL-1 stored on a slant at 4°C, inoculate it on the activation medium, and cultivate it for 18 hours. Transfer the well-grown strains to 10L fermentation medium, then add 50mL of EM bacteria solution, and cultivate at 30-35°C for 20 hours to obtain fermentation broth or dried fermentation broth. The number of bacteria in the bacterial agent can reach 1×10 10 ~9×10 12 CFU / g. The activation medium is LB medium, and the components of the fermentation medium are: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0; the composition of the fermentation medium is: according to 200 liters, the composition ratio is: KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH; The ve...

Embodiment 2

[0042] Embodiment 2 Preparation of compound biological bacterial fertilizer

[0043] Preparation of fermentation broth:

[0044] Activate the phosphate-solubilizing bacterium Bacillus subtilis HL-1 stored on a slant at 4°C, inoculate it on the activation medium, and cultivate it for 36 hours. Transfer the well-grown strains to 10L fermentation medium, then add 50mL of EM bacteria solution, and cultivate at 30-35°C for 20 hours to obtain fermentation broth or dried fermentation broth. The number of bacteria in the bacterial agent can reach 1×10 10 ~9×10 12 CFU / g. The activation medium is LB medium, and the components of the fermentation medium are: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0; the composition of the fermentation medium is: according to 200 liters, the composition ratio is: KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH; The ve...

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Abstract

The invention relates to a biological bacterial fertilizer, a preparation method and its application in soil phosphorus solution, and belongs to the technical field of biological fertilizers. The biological bacterial fertilizer provided by the present invention is compounded by phosphate-dissolving bacteria and EM bacterial liquid. The biological bacterial fertilizer utilizes the synergistic effect of effective microorganisms in the phosphorus-dissolving bacteria and EM bacterial liquid. On the one hand, it can dissolve the deposited phosphorus in the soil. On the other hand, the acidic products of the effective microbial metabolism of the EM bacteria liquid can change the acid-base state on the surface of phosphorus deposits, and promote the decomposition effect of phosphorus bacteria. Beneficial microorganisms such as bacteria and amino acids and other products produced by microbial metabolism can cooperate with phosphorus-solubilizing bacteria to balance the nitrogen-phosphorus ratio in the soil and make crops grow better.

Description

technical field [0001] The invention relates to a biological bacterial fertilizer, a preparation method and its application in soil phosphorus solution, and belongs to the technical field of biological fertilizers. Background technique [0002] Phosphorus and potassium are essential nutrients for plant growth and development. It is of great significance to study the availability of phosphorus and potassium in soil to improve soil fertility. However, phosphorus is one of the least soluble mineral nutrients in the soil liquid phase. The chemical form of phosphorus absorbed by plants is mainly H 2 PO 4 - and HPO 4 2- , but the phosphorus in the soil mainly exists in the form of insoluble primary minerals, which are difficult to be directly absorbed and utilized by crops under normal circumstances. At present, people usually meet the demand of plants for phosphorus by applying phosphorus fertilizer. Phosphate radicals are chemically active. After being applied to the soil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/80C05F17/20C12N1/20C12N1/16C12N1/14A01C21/00C12R1/80C12R1/66C12R1/07C12R1/125C12R1/22C12R1/10C12R1/01
CPCA01C21/005C05F11/08C05G3/00C12N1/14C12N1/16C12N1/20C05G3/80Y02W30/40
Inventor 许家兴贺爱永吴真胡磊夏军徐宁徐继明王新风
Owner 内蒙古力能肥业生物科技有限公司
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