Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological bacterial fertilizer as well as preparation method and application thereof in soil phosphate solubility

A technology of biological bacterial fertilizer and phosphate-solubilizing bacteria, which is applied in biochemical equipment and methods, microorganism-based methods, fertilization methods, etc., can solve the problem of low efficiency of nitrogen fertilizer improvement, achieve good growth promotion effect, improve phosphorus-solubilizing effect, Fertilizer effect

Active Publication Date: 2018-03-23
内蒙古力能肥业生物科技有限公司
View PDF9 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the single use of phosphorus solubilizing bacteria in the soil is not efficient in increasing the nitrogen fertilizer in the soil, and the synergistic effect of the EM bacteria solution and the phosphorus solubilizing bacteria is used to improve the content of phosphorus and nitrogen fertilizers in the soil. Effect; the present invention also provides a kind of carrier of this compound bacterial fertilizer simultaneously

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological bacterial fertilizer as well as preparation method and application thereof in soil phosphate solubility
  • Biological bacterial fertilizer as well as preparation method and application thereof in soil phosphate solubility
  • Biological bacterial fertilizer as well as preparation method and application thereof in soil phosphate solubility

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032]The present invention also provides the preparation method of the above-mentioned biological bacterial fertilizer. The above-mentioned preparation method of the biological bacterial fertilizer comprises the following steps: Step 1, after activating the phosphate-solubilizing bacteria, inoculate it on the activated medium and cultivate it for 12-36 hours; The second step is to select the strains on the activated medium, inoculate them in the fermentation medium, then add the EM stock solution to carry out fermentation, the temperature is 30-35°C, and the fermentation time is 12-36h. After fermentation, the fermentation liquid is obtained; the third step , the biological fertilizer obtained after mixing the fermentation broth with the adsorbent carrier. Described activation culture medium is calculated according to 1 liter composition ratio and is: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0~8.0; fermentation med...

Embodiment 1

[0034] Embodiment 1 Preparation of compound biological bacterial fertilizer

[0035] Preparation of fermentation broth:

[0036] Activate the phosphate-solubilizing bacterium Bacillus subtilis HL-1 stored on a slant at 4°C, inoculate it on the activation medium, and cultivate it for 18 hours. Transfer the well-grown strains to 10L fermentation medium, then add 50mL of EM bacteria solution, and cultivate at 30-35°C for 20 hours to obtain fermentation broth or dried fermentation broth. The number of bacteria in the bacterial agent can reach 1×10 10 ~9×10 12 CFU / g. The activation medium is LB medium, and the components of the fermentation medium are: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0; the composition of the fermentation medium is: according to 200 liters, the composition ratio is: KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH; The...

Embodiment 2

[0042] Embodiment 2 Preparation of compound biological bacterial fertilizer

[0043] Preparation of fermentation broth:

[0044] Activate the phosphate-solubilizing bacterium Bacillus subtilis HL-1 stored on a slant at 4°C, inoculate it on the activation medium, and cultivate it for 36 hours. Transfer the well-grown strains to 10L fermentation medium, then add 50mL of EM bacteria solution, and cultivate at 30-35°C for 20 hours to obtain fermentation broth or dried fermentation broth. The number of bacteria in the bacterial agent can reach 1×10 10 ~9×10 12 CFU / g. The activation medium is LB medium, and the components of the fermentation medium are: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0; the composition of the fermentation medium is: according to 200 liters, the composition ratio is: KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH; The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biological bacterial fertilizer, a preparation method and application thereof in soil phosphate solubility and belongs to the technical field of bio-fertilizers. The biological bacterial fertilizer disclosed by the invention is prepared by compounding phosphate solubilizing bacteria and an EM bacterial solution; the biological bacterial fertilizer utilizes a synergisticeffect of effective microorganisms in the phosphate solubilizing bacteria and the EM bacterial solution, so that on one hand, sedimentary phosphor in soil can be dissolved; on the other hand, an acid-base state on the surface of phosphorus deposit can be changed by an acidic product which is generated after metabolism of the effective microorganisms of the EM bacterial solution, and a decomposingeffect of the phosphate solubilizing bacteria is improved; meanwhile, the EM bacterial solution contains rich beneficial microorganisms of actinomycetes, saccharomycetes, photosynthetic bacteria and the like and products of amino acid and the like generated after microbial metabolism; the EM bacterial solution can mutually cooperate with the phosphate solubilizing bacteria to make a nitrogen / phosphorus ratio in soil reach balance, so that a crop growth effect is better.

Description

technical field [0001] The invention relates to a biological bacterial fertilizer, a preparation method and its application in soil phosphorus solution, and belongs to the technical field of biological fertilizers. Background technique [0002] Phosphorus and potassium are essential nutrients for plant growth and development. It is of great significance to study the availability of phosphorus and potassium in soil to improve soil fertility. However, phosphorus is one of the least soluble mineral nutrients in the soil liquid phase. The chemical form of phosphorus absorbed by plants is mainly H 2 PO 4 - and HPO 4 2- , but the phosphorus in the soil mainly exists in the form of insoluble primary minerals, which are difficult to be directly absorbed and utilized by crops under normal circumstances. At present, people usually meet the demand of plants for phosphorus by applying phosphorus fertilizer. Phosphate radicals are chemically active. After being applied to the s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C05F11/08C05G3/00C05G3/04C12N1/20C12N1/16C12N1/14A01C21/00C12R1/80C12R1/66C12R1/07C12R1/125C12R1/22C12R1/10C12R1/01C05G3/80
CPCA01C21/005C05F11/08C05G3/00C12N1/14C12N1/16C12N1/20C05G3/80Y02W30/40
Inventor 许家兴贺爱永吴真胡磊夏军徐宁徐继明王新风
Owner 内蒙古力能肥业生物科技有限公司
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com