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Mouse ovary separation and oocyte culture device

A technology of oocyte and culture device, which is applied in the field of reproductive biology, can solve the problems such as the decline of oocyte survival rate, and achieve the effect of complete equipment, efficient collection and efficient extraction

Inactive Publication Date: 2018-03-30
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of ovarian crushing releases a lot of impurities that are not conducive to the survival of oocytes. These impurities directly contact with oocytes and seriously affect the necessary living environment of oocytes, so that the survival rate of oocytes gradually decreases.

Method used

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  • Mouse ovary separation and oocyte culture device
  • Mouse ovary separation and oocyte culture device
  • Mouse ovary separation and oocyte culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A mouse ovary isolation and oocyte culture device, the device includes a kit and a kit, the kit includes 2g / L collagenase, 2 g / L hyaluronidase, containing 300 U / mL of penicillin and 300 µg / mL streptomycin MII culture medium, MII culture medium was purchased from Sigma Company. The equipment box includes an ovarian stripping needle, an egg suction device and a detachable cell sieve.

[0032] In addition to the ovarian stripping needle, egg suction device, and cell screen, the equipment box also includes a 3.5 cm cell culture dish and Ep tube. In addition, you need to bring your own surgical instruments and a high-pressure 200 µl pipette tip. The ovum suction needle 6, the micro filter 9 and the high-pressure 200 µl pipette tip 8 are connected through the ovum suction tube 7. The actual egg suction tube is connected by two transparent rubber tubes with a diameter of 3 mm and a length of 20 cm and 70 cm, respectively, and a micro-filter

[0033] The detachable cell siev...

Embodiment 2

[0042] Embodiment 2 The present invention compares with previous method:

[0043] The previous method: after the mice were anesthetized and sacrificed by cervical dislocation, the ovaries were removed by laparotomy, placed in 190 μl MB culture drop, and the ovarian follicles were repeatedly broken with a 1 ml syringe. Then the naked oocytes were sucked into another MB culture medium one by one with egg suction. This process time can be as long as 1-4 hours, and because there is no filter device in the oviduct, the quality of life of the oocyte is seriously affected. In this way, it is difficult for the oocytes obtained by chance to wash all the granulosa cells around them.

[0044] The previous method used the MB culture medium produced by Life technologies, the full name is Waymouth’s MB752 / 1 Medium (1X), liquid + L-Glutamine. Product number W1625.

[0045]

previous method

This kit

summary

Egg retrieval time

1-4 hours

15-25 minutes

25...

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Abstract

The invention belongs to the field of reproductive biology and particularly relates to a mouse ovary separation and oocyte culture device, comprising a kit and an instrument box; the kit includes collagenase, hyaluronidase and MII culture solution containing penicillin and streptomycin; the instrument box comprises an ovary stripping needle, an ovum suction device and a releasable cell sieve. Themouse ovary separation and oocyte culture device has the advantages that separation and extraction time for mouse oocytes and granular cells around them can be shortened, the separation and extractionprocesses are simplified, and extraction quality and efficiency for oocytes and granular cells are greatly improved.

Description

technical field [0001] The invention belongs to the field of reproductive biology, in particular to a mouse ovary isolation and oocyte culture device. Background technique [0002] Mouse oocyte sampling and culture technology is an important means to study the mechanism of mouse early embryo development and its biological characteristics. The application of in vitro culture technology to the study of mouse early embryology has many advantages: (1) It can avoid multiple diseases in the body. Influenced by various factors, it is beneficial to use physical, chemical or biological factors to explore the life activity rules of oocytes and granulosa cells in the ovary. (2) It is beneficial to study the structure and function of oocytes and granulosa cells, as well as the localization of certain proteins in them. (3) It is also beneficial to screen agonists or inhibitors that affect the growth and development of oocytes, etc. [0003] Mouse oocyte culture techniques are different...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06C12N5/075
CPCC12M29/04C12M33/04C12N5/0609C12N2509/10
Inventor 邓欣白璐李森陈颖
Owner 中国医科大学
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