Matrix-assisted laser desorption-gas phase polarization-induced proton transfer mass spectrometry

Abstract

The invention discloses a matrix-assisted laser desorption-gas-phase polarization induced proton transfer mass spectrum. A protonated analyte is produced through combination of a matrix-assisted laserdesorption technology with a proton transfer reaction technology. Gas-state analyte molecules are formed through matrix-assisted laser desorption, and protonation of the analyte is realized through collision and reaction of the gas-state analyte molecules with excited-state highly-polarized dichloromethane. According to the method, the desorption and ionization processes of the analyte are separated, so that ionization of the analyte is independent of the effects of a solid-phase matrix, and wider desorption, ionization and quality analysis of organic matter and biological molecules can be realized. The matrix application foundation and selectable range are broadened and new ion formation mechanisms and technologies are provided.

Description

[0001] Technical field [0002] The invention proposes a new soft ionization mass spectrum mode. This mode first uses matrix-assisted laser desorption of various organic substances and biomolecules, and uses the stimulated polarization of specific gas-phase molecules to selectively transfer protons to their polar groups to achieve protonation for their mass analysis. Provides new mass spectrometry techniques and methods. Background technique [0003] Existing soft ionization mass spectrometry techniques, such as electrospray and matrix-assisted laser desorption ionization mass spectrometry techniques, are important means for detecting organic substances and biomolecules. These soft ionization techniques have one thing in common: two critical steps, gasification and ionization of biomolecules, are difficult to distinguish because they occur simultaneously or coupled, thus bringing many technical and application limitations. [0004] In order to illustrate the above problems, ...

AI Technical Summary

Problems solved by technology

Although matrix-assisted laser desorption ionization mass spectrometry has high sensitivity (10 -15 mol), but only for some specific substances

The main limitation of the current matrix-assisted laser desorption ionization mass spectrometry technology is the lack of a wide range of effective matrix to desorb and ionize the analyte at the same time, and many matrices (such as 2,5-dihydroxybenzoic acid, 2,5-DHB) have certain carcinogenic properties. In addition, due to the lack of good miscibility between the analyte, the matrix and the doped salt and the solution, the sample preparation process is often very complicated, and the professional requirements for the instrument operator are very high

These problems seriously restrict the application of matrix-assisted laser desorption ionization mass spectrometry

Images