Matrix-assisted laser desorption-gas-phase polarization induced proton transfer mass spectrum
A matrix-assisted laser and polarization-induced technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high professional requirements for instrument operators, complicated sample preparation process, and restrictions on the application of matrix-assisted laser desorption ionization mass spectrometry technology , to achieve the effect of expanding the base and the range of options
Active Publication Date: 2018-04-03
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Problems solved by technology
Although matrix-assisted laser desorption ionization mass spectrometry has high sensitivity (10 -15 mol), but only for some specific substances
The main limitation of the current matrix-assisted laser desorption ionization mass spectrometry technology is the lack of a wide range of effective matrix to desorb and ionize the analyte at the same time, and many matrices (such as 2,5-dihydroxybenzoic acid, 2,5-DHB) have certain carcinogenic properties. In addition, due to the lack of good miscibility between the analyte, the matrix and the doped salt and the solution, the sample preparation process is often very complicated, and the professional requirements for the instrument operator are very high
These problems seriously restrict the application of matrix-assisted laser desorption ionization mass spectrometry
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[0013] The main part of matrix-assisted laser desorption-gas phase polarization-induced proton transfer mass spectrometry consists of vacuum ultraviolet light source (1), desorption ionization chamber (2), sample chamber (3), pulse laser (4), ion selection chamber (5), ion The collision dissociation chamber (6) and the mass spectrometry chamber (7) consist of seven parts. The specific implementation method is:
[0014] One, vacuum ultraviolet krypton lamp (13) produces vacuum ultraviolet light (14) of energy of 10 and 10.6 electron volts and enters desorption ionization chamber (2) through magnesium fluoride lens (15);
[0015] Two, methylene chloride gas (16) enters desorption ionization chamber (2) by methylene chloride gas sampling valve (8);
[0016] 3. Vacuum ultraviolet light (14) irradiates dichloromethane gas (16) to generate highly polarized dichloromethane in an excited state;
[0017] 4. The substrate automatic transporter (18) transmits the substrate (19) for dep...
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The invention discloses a matrix-assisted laser desorption-gas-phase polarization induced proton transfer mass spectrum. A protonated analyte is produced through combination of a matrix-assisted laserdesorption technology with a proton transfer reaction technology. Gas-state analyte molecules are formed through matrix-assisted laser desorption, and protonation of the analyte is realized through collision and reaction of the gas-state analyte molecules with excited-state highly-polarized dichloromethane. According to the method, the desorption and ionization processes of the analyte are separated, so that ionization of the analyte is independent of the effects of a solid-phase matrix, and wider desorption, ionization and quality analysis of organic matter and biological molecules can be realized. The matrix application foundation and selectable range are broadened and new ion formation mechanisms and technologies are provided.
Description
Technical field [0001] The invention proposes a new soft ionization mass spectrum mode. This mode first uses matrix-assisted laser desorption of various organic substances and biomolecules, and uses the stimulated polarization of specific gas-phase molecules to selectively transfer protons to their polar groups to achieve protonation for their mass analysis. Provides new mass spectrometry techniques and methods. Background technique [0002] Existing soft ionization mass spectrometry techniques, such as electrospray and matrix-assisted laser desorption ionization mass spectrometry techniques, are important means for detecting organic substances and biomolecules. These soft ionization techniques have one thing in common: two critical steps, gasification and ionization of biomolecules, are difficult to distinguish because they occur simultaneously or coupled, thus bringing many technical and application limitations. [0003] In order to illustrate the above problems, matrix-...
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IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 束继年汪海林李震杨波张鹏
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI