Quality determination method for HPLC-DAD fingerprint spectrum of eutrema wasabi maxim medicinal material

A fingerprint and quality measurement technology, which is applied in the field of quality control of traditional Chinese medicine, can solve problems such as lack of medicinal materials, and achieve the effects of reasonable detection time, good separation, and accurate quality control

Active Publication Date: 2018-04-20
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as the medicinal materials of gooseberry are concerned, the entire medicina...

Method used

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  • Quality determination method for HPLC-DAD fingerprint spectrum of eutrema wasabi maxim medicinal material
  • Quality determination method for HPLC-DAD fingerprint spectrum of eutrema wasabi maxim medicinal material
  • Quality determination method for HPLC-DAD fingerprint spectrum of eutrema wasabi maxim medicinal material

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Embodiment 1

[0040] The present embodiment proposes a kind of HPLC-DAD fingerprint spectrum quality determination method of the wild gooseberry medicinal material, and the steps are as follows:

[0041] (A) Preparation of the test solution: take 0.5g of the behenicum medicinal material, put it in 20ml of methanol, after ultrasonic treatment (power 250W, frequency 45KHz) for 0.5h, let it cool to 10°C, shake well, and use 0.22 After filtering with a microporous membrane of 1 μm, take the continued filtrate as the solution of the test sample and set it aside;

[0042] (B) Preparation of reference solution: take 3-butenyl isothiocyanate reference substance, 5-methylthiopentyl-isothiocyanate reference substance, 6-methylthiohexyl-isothiocyanate reference substance product, dissolved in methanol, and prepared as a reference substance containing 80 μg 3-butenyl isothiocyanate, 80 μg 5-methylthiopentyl-isothiocyanate and 80 μg 6-methylthiohexyl-isothiocyanate per 1 ml solution, ready to use;

[...

Embodiment 2

[0056] The present embodiment proposes a kind of HPLC-DAD fingerprint spectrum quality determination method of the wild gooseberry medicinal material, and the steps are as follows:

[0057] (A) Preparation of the test solution: Take 1.0g of the behenicum medicinal material, put it in 40ml of methanol, after ultrasonic treatment (power 250W, frequency 45KHz) for 1h, let it cool to 30°C, shake well, and use 0.22um After filtering with a microporous membrane, get the continued filtrate as the test solution and stand by;

[0058] (B) Preparation of reference solution: take 3-butenyl isothiocyanate reference substance, 5-methylthiopentyl-isothiocyanate reference substance, 6-methylthiohexyl-isothiocyanate reference substance product, dissolved in methanol, and prepared as a reference substance containing 80 μg 3-butenyl isothiocyanate, 80 μg 5-methylthiopentyl-isothiocyanate and 80 μg 6-methylthiohexyl-isothiocyanate per 1 ml solution, ready to use;

[0059] (C) Determination: Dr...

Embodiment 3

[0072] The present embodiment proposes a kind of HPLC-DAD fingerprint spectrum quality determination method of the wild gooseberry medicinal material, and the steps are as follows:

[0073] (A) Preparation of the test solution: Take 2.0g of the behenicum medicinal material, put it in 80ml of methanol, after ultrasonic treatment (power 250W, frequency 45KHz) for 0.5h, let cool to 25°C, shake well, and use 0.22 After filtering with a microporous membrane of 1 μm, take the continued filtrate as the solution of the test sample and set it aside;

[0074] (B) Preparation of reference solution: take 3-butenyl isothiocyanate reference substance, 5-methylthiopentyl-isothiocyanate reference substance, 6-methylthiohexyl-isothiocyanate reference substance product, dissolved in methanol, and prepared as a reference substance containing 80 μg 3-butenyl isothiocyanate, 80 μg 5-methylthiopentyl-isothiocyanate and 80 μg 6-methylthiohexyl-isothiocyanate per 1 ml solution, ready to use;

[007...

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Abstract

The invention discloses a quality determination method for an HPLC-DAD fingerprint spectrum of a eutrema wasabi maxim medicinal material. The method includes the steps: respectively preparing a test sample solution and a control substance solution prepared from a 3-butylene isothiocyanate control substance, a 5-methylthiopentyl-isothiocyanate control substance and a 6-methylthiohexyl-isothiocyanate control substance, and injecting the two solutions into a high performance liquid chromatographic instrument, and determining by gradient elution via a high performance liquid chromatography method,to obtain the fingerprint spectrum of the eutrema wasabi maxim medicinal material. According to the method, the eutrema wasabi maxim medicinal material is determined by gradient elution via the highperformance liquid chromatography method; the fingerprint spectrum of the eutrema wasabi maxim medicinal material is constructed, characteristic substances contained in the eutrema wasabi maxim medicinal material are truly reflected, and isothiocyanate compounds in the eutrema wasabi maxim medicinal material are analyzed and determined; and at the same time, the method is used as a quality controlmethod of the eutrema wasabi maxim medicinal material, and has the characteristics of good separation effect and high sensitivity.

Description

technical field [0001] The invention relates to an HPLC-DAD fingerprint quality determination method for the Chinese gourd medicinal material, in particular to an HPLC-DAD fingerprint quality evaluation method for the Chinese gourd medicinal material, and belongs to the field of traditional Chinese medicine quality control. Background technique [0002] Beancurd ( Eutrema wasabi Maxim), also known as wasabi, is a perennial herbaceous plant of the genus Behenus in the family Brassicaceae. Behenicum is rich in mustard oil, which ranks first among all kinds of spicy seasonings for its unique aroma, pungent, and sticky properties. In addition, it also has the functions of helping digestion, eliminating toxins, resisting active oxygen free radicals, and antagonizing a variety of human body. The role of disease germs, has a high edible health value. The main spicy ingredient isothiocyanate (Isothiocyanate) has antibacterial, anticancer, antioxidation and other pharmacological e...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/64
CPCG01N30/02G01N30/64
Inventor 李启发王晨曦张巨琼张佳卉徐仕银刘丁文焕松夏柯陈冲
Owner CHENGDU PUSH BIOLOGICAL TECH
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