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A ratiometric fluorescent probe of morpholinoguanidine based on copper nanocluster/fluorescein complex

A technology of ratiometric fluorescent probes and copper nanoclusters, applied in the field of preparation of fluorescent nanomaterials and visualization probes, achieving the effects of high product sensitivity, simple preparation process, and low preparation cost

Active Publication Date: 2019-10-22
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no ratiometric fluorescence method for the detection of morpholine guanidine, the preparation of morpholine guanidine ratiometric fluorescent probes based on copper nanoclusters / fluorescein complexes, and the relevant domestic and foreign literature on the ratiometric fluorescence detection of morpholine guanidine. Patent report

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  • A ratiometric fluorescent probe of morpholinoguanidine based on copper nanocluster/fluorescein complex
  • A ratiometric fluorescent probe of morpholinoguanidine based on copper nanocluster/fluorescein complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation process of a morpholine-guanidine ratiometric fluorescent probe based on copper nanocluster / fluorescein complexes involved in this example and the schematic diagram of the principle of morpholineguanidine ratiometric fluorescence detection can be found in figure 1 As shown, the specific process steps are:

[0018] Preparation of CuNCs: Prepare 50 ml of copper chloride aqueous solution with a molar concentration of 0.1 mol / L, add 0.25 g of bovine serum albumin, and prepare a homogeneous solution under magnetic stirring, then add 1 ml of 50 wt% hydrazine hydrate dropwise, at room temperature The reaction was carried out for 6 hours. After the reaction was completed, it was extracted with acetone, the suspension was sucked and centrifuged, washed with ethanol, and dried in vacuum to obtain CuNCs;

[0019] Preparation of CuNCs / fluorescein complex: Prepare 50 ml of CuNCs aqueous solution with a mass concentration of 1 mg / ml, add 5 mg of nitrogen-hydroxysuccin...

Embodiment 2

[0023]In this example, the specific process steps for the preparation of CuNCs and CuNCs / fluorescein complexes are the same as those in Example 1. 20 ml of 1 mg / ml CuNCs / fluorescein complex aqueous solution was prepared, and the molar concentration of 20 mmol / ml was added dropwise. Morpholine guanidine solution forms a series of homogeneous mixed solutions under room temperature and magnetic stirring, and uses a fluorescence spectrometer to measure the fluorescence emission spectra of the homogeneous solution at different molar concentrations of morpholine guanidine coexistence, and fits the fluorescence emission peak intensities of CuNCs and fluorescein Ratio I 荧光素 / I CuNCs and the linear relationship between the concentration of morpholine guanidine in the analyte, a ratio fluorescent probe that can quantitatively detect morpholine guanidine is constructed, and the concentration range of the probe detection morpholine guanidine in the analyte is 0.1 to 1 millimolar / L, the ...

Embodiment 3

[0025] In this example, the specific process steps for the preparation of CuNCs and CuNCs / fluorescein complexes are the same as in Example 1. 50 ml of 1 mg / ml CuNCs / fluorescein complex aqueous solution was prepared, and the molar concentration of 50 mmol / ml was added dropwise. Morpholine guanidine solution was mixed at room temperature and under magnetic stirring to form a series of homogeneous mixed solutions. Fluorescence emission spectra of the homogeneous solutions at different molar concentrations of morpholine guanidine were measured by fluorescence spectrometer, and the fluorescence emission peaks of CuNCs and fluorescein were fitted. Intensity Ratio I 荧光素 / I CuNCs and the linear relationship between the concentration of morpholine guanidine in the analyte, and then construct a ratiometric fluorescent probe that can quantitatively detect morpholine guanidine. mol / L, the detection limit of morpholine guanidine is 0.1 mmol / L; transfer a series of the above-mentioned homo...

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Abstract

The invention belongs to the technical field of preparation of fluorescent nano materials and visual probes, and particularly relates to a preparation method of a moroxydine ratiometric fluorescent probe based on a copper nano-cluster / fluorescein composite. The method mainly includes the steps of: 1) preparing bovine serum albumin stabilized red fluorescence emitting copper nano-cluster through ahydrothermal method; 2) preparing the copper nano-cluster / fluorescein nano composite through a carboxamide coupling method; 3) adding the moroxydine molecules, which is combined with the BSA, so as toinduce red fluorescence quenching of the copper nano-cluster, while green fluorescence of the fluorescein is not significantly influenced; 4) establishing a linear relationship between the ratio of intensities of fluorescence-emission peaks of the copper nano-cluster and the fluorescein and the molar concentration of the moroxydine, and a correspondence relationship between the molar concentration of the moroxydine and the fluorescent color of same, thus preparing the ratiometric fluorescent visual probe for detection of the moroxydine. The probe is simple in preparation method and low in preparation cost, has high sensitivity, and is suitable for ratiometric fluorescent visual detection of the moroxydine in a biosample.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of fluorescent nanomaterials and visualization probes, in particular to a method for preparing a morpholine guanidine ratio fluorescent probe based on a copper nanocluster / fluorescein complex, and the prepared probe can be used for morpholine guanidine Ratiometric Fluorescence Visualization Assay. Background technique: [0002] Guanidine compounds exist in physiological fluids and tissues, play an important role in regulating the functions of organisms, and can also be widely used in the pharmaceutical industry as pharmaceutical ingredients. As one of the important guanidine compounds, morpholine guanidine (or virus spirit) has various antiviral effects on RNA and DNA viruses. Morpholinidine has been proven effective in the treatment of hepatitis C, flu symptoms, varicella zoster, measles, mumps, tobacco mosaic virus and other diseases. Conventional methods for detecting morpholine guanidin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 桂日军王本乾王宗花
Owner QINGDAO UNIV
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