Moroxydine ratiometric fluorescent probe based on copper nano-cluster/fluorescein composite
A ratio fluorescent probe and copper nanocluster technology is applied in the field of preparation of fluorescent nanomaterials and visualized probes to achieve the effects of low preparation cost, high product sensitivity and simple preparation process
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Embodiment 1
[0017] The preparation process of a morpholine-guanidine ratiometric fluorescent probe based on copper nanocluster / fluorescein complexes involved in this example and the schematic diagram of the principle of morpholineguanidine ratiometric fluorescence detection can be found in figure 1 As shown, the specific process steps are:
[0018] Preparation of CuNCs: Prepare 50 ml of copper chloride aqueous solution with a molar concentration of 0.1 mol / L, add 0.25 g of bovine serum albumin, and prepare a homogeneous solution under magnetic stirring, then add 1 ml of 50 wt% hydrazine hydrate dropwise, at room temperature The reaction was carried out for 6 hours. After the reaction was completed, it was extracted with acetone, the suspension was sucked and centrifuged, washed with ethanol, and dried in vacuum to obtain CuNCs;
[0019] Preparation of CuNCs / fluorescein complex: Prepare 50 ml of CuNCs aqueous solution with a mass concentration of 1 mg / ml, add 5 mg of nitrogen-hydroxysuccin...
Embodiment 2
[0023] In this example, the specific process steps for the preparation of CuNCs and CuNCs / fluorescein complexes are the same as those in Example 1. 20 ml of 1 mg / ml CuNCs / fluorescein complex aqueous solution was prepared, and the molar concentration of 20 mmol / ml was added dropwise. Morpholine guanidine solution forms a series of homogeneous mixed solutions under room temperature and magnetic stirring, and uses a fluorescence spectrometer to measure the fluorescence emission spectra of the homogeneous solution at different molar concentrations of morpholine guanidine coexistence, and fits the fluorescence emission peak intensities of CuNCs and fluorescein Ratio I荧光素 / I CuNCs and the linear relationship between the molar concentration of morpholine guanidine, a ratio fluorescent probe that can quantitatively detect morpholine guanidine is constructed, and the molar concentration range of the probe detection morpholine guanidine is 0.1~1 mmol / L, and the molar concentration of mor...
Embodiment 3
[0025] In this example, the specific process steps for the preparation of CuNCs and CuNCs / fluorescein complexes are the same as in Example 1. 50 ml of 1 mg / ml CuNCs / fluorescein complex aqueous solution was prepared, and the molar concentration of 50 mmol / ml was added dropwise. Morpholine guanidine solution was mixed at room temperature and under magnetic stirring to form a series of homogeneous mixed solutions. Fluorescence emission spectra of the homogeneous solutions at different molar concentrations of morpholine guanidine were measured by fluorescence spectrometer, and the fluorescence emission peaks of CuNCs and fluorescein were fitted. Intensity Ratio I 荧光素 / I CuNCs and the linear relationship between the molar concentration of morpholine guanidine, and then construct the ratio fluorescent probe that can quantitatively detect morpholine guanidine, the molar concentration range of this probe detection morpholine guanidine is 1~10 mmol / L, morpholine guanidine The limit of...
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