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A detection method, device and terminal equipment for off-target sites

A detection method and off-target technology, applied in the field of genomics, can solve the problems affecting the safety of CRISPR experiments and the inaccurate evaluation method of off-target effects, etc.

Active Publication Date: 2021-09-10
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the embodiment of the present invention provides a detection method, device and terminal equipment for off-target sites, so as to solve the problem that the existing evaluation method of off-target effects using CRISPR-Cas9 technology for multi-cells is not accurate enough, thus affecting the CRISPR experiment process security issues in

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  • A detection method, device and terminal equipment for off-target sites
  • A detection method, device and terminal equipment for off-target sites
  • A detection method, device and terminal equipment for off-target sites

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Embodiment 1

[0026] figure 1 A flow chart of a detection method for an off-target site provided by an embodiment of the present invention is shown, and the details are as follows:

[0027] Step S101, selecting a gene sample, the gene sample includes a gene sample of an offspring and a parent gene sample of the gene sample of the offspring.

[0028] Specifically, the gene samples of the target embryonic cells are obtained, including the offspring gene samples of the target embryonic cells and the parental gene samples of the offspring gene samples. In the embodiment of the present invention, the selected gene samples include offspring gene samples and the parent gene samples of the offspring gene samples. Mark) site, it is not compared with the information in the database, but compared with the InDel site information in the parental parent (that is, the parental gene sample), so as to realize the personalized search for the target cell genome Possible off-target (off-target site). Among ...

Embodiment 2

[0061] Corresponding to the detection method of the off-target site described in the above examples, image 3 A structural block diagram of the off-target site detection device provided by the embodiment of the present invention is shown, and for the convenience of description, only the parts related to the embodiment of the present invention are shown.

[0062] refer to image 3 , the detection device of the off-target site includes: a sample selection unit 31, a sample comparison unit 32, a result generation unit 33, and a result display unit 34, wherein:

[0063] A sample selection unit 31, configured to select a gene sample, the gene sample comprising a gene sample of a progeny and a parent gene sample of the gene sample of the progeny;

[0064] The sample comparison unit 32 is used to process the genetic samples of the offspring and the genetic samples of the parents through whole genome sequencing, and compare the sequenced genetic samples of the offspring and the genet...

Embodiment 3

[0090] Figure 4 It is a schematic diagram of a terminal device provided by an embodiment of the present invention. Such as Figure 4 As shown, the terminal device 4 of this embodiment includes: a processor 40, a memory 41, and a computer program 42 stored in the memory 41 and operable on the processor 40, such as a detection program for off-target sites. When the processor 40 executes the computer program 42, it realizes the steps in the above embodiments of the detection method for each off-target site, for example figure 1 Steps 101 to 104 are shown. Alternatively, when the processor 40 executes the computer program 42, it realizes the functions of the modules / units in the above-mentioned device embodiments, for example image 3 Function of units 31 to 34 shown.

[0091] Exemplarily, the computer program 42 can be divided into one or more modules / units, and the one or more modules / units are stored in the memory 41 and executed by the processor 40 to complete this inven...

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Abstract

The present invention is applicable to the technical field of genomics, and provides a detection method, device and terminal equipment for off-target sites, including: selecting a gene sample, the gene sample includes a progeny gene sample and a parental gene of the progeny gene sample Samples: respectively process the progeny gene sample and the parent gene sample by whole genome sequencing, and compare the sequenced progeny gene sample and the parent gene sample according to specified comparison rules; Perform format conversion on the result of the comparison to generate an comparison result file in a specified format; display the comparison result file in the specified format through the integrated genomics viewer IGV, so that the user can confirm the number of off-targets and off-target sites. Through the above method, the safety during the CRISPR experiment can be improved.

Description

technical field [0001] The invention belongs to the technical field of genomics, and in particular relates to an off-target site detection method, device and terminal equipment. Background technique [0002] CRISPR (Clustered regularly interspaced short palindromic repeats), known as regular clustered interspaced short palindromic repeats, is a system used by bacteria to protect themselves against viruses and a genetic weapon against attackers. CRISPR-Cas9 is a genome editing technology that can treat a variety of diseases through DNA cutting technology, but this technology is not perfect. When used, it sometimes produces insertions and indels at unanticipated off-target sites. Given that unanticipated gene cuts may lead to unintended mutations, detection of off-target sites during CRISPR-Cas9 gene editing is critical. At present, there are many evaluation methods for off-target effects of CRISPR-Cas9 technology, such as software prediction (CRISPR Design), GUIDE-seq, Dige...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B30/10G16B40/00G16B50/10G16B45/00
CPCG16B30/00G16B45/00
Inventor 贺建奎陈凯婧陈杨冉
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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