A kind of fat-soluble gardenia blue pigment and preparation method thereof

A gardenia blue pigment and fat-soluble technology, applied in the field of biochemistry, can solve the problems of unclear pigment composition and structure, and unimproved light stability

Active Publication Date: 2021-08-20
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through patent search, the authorized patent "a method for preparing alcohol-soluble gardenia blue pigment derivatives" uses water-soluble gardenia blue pigment and acetic anhydride as raw materials, and prepares an alcohol soluble in absolute ethanol through esterification reaction Soluble gardenia blue pigment, but the pigment is still insoluble in organic solvents such as ethyl acetate, acetone, petroleum ether, etc., its light stability has not been improved, and the pigment composition and structure of the derivative are not clear

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  • A kind of fat-soluble gardenia blue pigment and preparation method thereof
  • A kind of fat-soluble gardenia blue pigment and preparation method thereof
  • A kind of fat-soluble gardenia blue pigment and preparation method thereof

Examples

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Effect test

Embodiment 1

[0046] Example 1 is used to illustrate the preparation method of the fat-soluble gardenia blue pigment provided by the present invention, and the amino acid methyl ester hydrochloride used in the implementation case is phenylalanine methyl ester hydrochloride.

[0047] Accurately weigh the reaction raw materials for preparing fat-soluble gardenia blue pigment according to the amounts listed in Table 1, and react according to the listed reaction temperature and reaction time.

[0048] The preparation conditions of table 1 fat-soluble gardenia blue pigment

[0049]

[0050] Embodiment 1, the preparation of fat-soluble gardenia blue pigment

[0051] According to the formula shown in Table 1, the following reaction steps are used to prepare fat-soluble gardenia blue pigment:

[0052] Preparation of fat-soluble gardenia blue pigment using genipin and phenylalanine methyl ester hydrochloride:

[0053] (1) Accurately weigh 2mmol genipin and 2.6mmol phenylalanine methyl ester hyd...

Embodiment 2

[0057] Embodiment 2, structure characterization of fat-soluble gardenia blue pigment

[0058] 2.1. Observing the microscopic morphology of the fat-soluble gardenia blue pigment prepared in Example 1 by scanning electron microscopy (SEM), the results are shown in figure 1 1. The prepared fat-soluble gardenia blue pigment is mainly in the form of spherical particles, and the particle size is about 100nm.

[0059]2.2. Weigh 0.1g of the fat-soluble gardenia blue pigment prepared in Example 1 and dissolve it in ethanol, dilute it by an appropriate multiple, use ethanol as a reference, and use a UV-Vis spectrophotometer (UV-Vis) to measure the pigment solution 400~ UV-Vis absorption spectrum in the 700nm interval.

[0060] 2.3, adopt Fourier transform infrared spectrometer (FTIR) and X-ray photoelectron spectrometer (XPS) to measure the characteristic functional group of the fat-soluble gardenia blue pigment that embodiment 1, 2 prepares, as Figure 2-7 And shown in Table 2-4.

...

Embodiment 3

[0068] Embodiment 3, fat-soluble gardenia blue pigment stability investigation

[0069] 3.1. Solubility investigation:

[0070] The fat-soluble gardenia blue pigment and the water-soluble gardenia blue pigment prepared in Example 1 were respectively placed in equal volumes of distilled water and various organic solvents, fully dissolved, and tested for solubility.

[0071] Table 5 Solubility of fat-soluble and water-soluble gardenia blue pigments in different solvents

[0072]

[0073] Note: √Easily soluble; ×Insoluble

[0074] 3.2. Light stability inspection

[0075] The stability of fat-soluble gardenia blue pigment and water-soluble gardenia blue pigment were investigated under the conditions of sunlight, direct light, indoor scattering and dark and light-proof (control) respectively. The light intensity is shown in Table 6:

[0076] Table 6 Light intensity of different light conditions

[0077]

[0078] The fat-soluble gardenia blue pigment powder and the water-...

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Abstract

The invention relates to a fat-soluble gardenia blue pigment and a preparation method thereof. The preparation method comprises the following steps: (1) Weighing genipin and amino acid methyl ester hydrochloride, dissolving them in ethanol, fully dissolving and shaking uniformly; 2) Put it in a water bath shaker at 40°C-70°C, and react under normal pressure at 150-220rpm in the presence of oxygen; (3) After the reaction is completed, the ethanol is removed by rotary evaporation to obtain the crude fat-soluble gardenia blue pigment; (4) Concentrate 1. Purifying crude fat-soluble gardenia blue pigment to obtain pure fat-soluble gardenia blue pigment. The fat-soluble gardenia blue pigment is a mixture, and the fat-soluble gardenia blue pigment is mainly in the form of spherical particles with a particle size of about 100 nm. Light and temperature stability are higher than water-soluble gardenia blue pigment.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a fat-soluble gardenia blue pigment and a preparation method thereof. Background technique [0002] Gardenia blue pigment is a natural blue pigment approved for use in my country since 1989. It uses the Rubiaceae plant gardenia fruit as raw material to transform into genipin through microbial fermentation or enzymatic biotransformation, or directly from genipin and Amino acids are prepared after a series of polymerization and rearrangement reactions, and can be widely used in food, medicine and cosmetics. [0003] The study on the stability of gardenia blue pigment found that it is easily soluble in water, has good dyeing performance, and has high stability to acid and alkali, high temperature and various commonly used food additives. However, since the pigment cannot be dissolved in organic solvents and oils such as absolute ethanol, ethyl acetate, acetone, petroleum ethe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/04A23L5/43A61K47/22A61K8/49C09B57/00
CPCA23V2002/00A61K8/4926A61K47/22A23L5/43C07D221/04C09B57/00A23V2200/044
Inventor 刘国荣王成涛郜亚昆
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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