Extraction and purification method for matrine and oxymatrine in Sophora flavescens

A technology of oxymatrine and matrine, which is applied in the field of extraction and preparation of natural active substances, achieves the effects of easy industrialization, simple process, and environmental friendliness

A technology of oxymatrine and matrine, which is applied in the field of extraction and preparation of natural active substances, achieves the effects of easy industrialization, simple process, and environmental friendliness

CN107987079AInactive Publication Date: 2018-05-04ZHONGKAI UNIV OF AGRI & ENG
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  • Extraction and purification method for matrine and oxymatrine in Sophora flavescens
  • Extraction and purification method for matrine and oxymatrine in Sophora flavescens
  • Extraction and purification method for matrine and oxymatrine in Sophora flavescens

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Experimental program
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Effect test

Embodiment 1

[0041] 1. Raw materials and instruments (other similar raw materials and instruments can be used, the present invention is not limited to products produced by specific manufacturers)

[0042] Glycidyl methacrylate (GMA)>97.0%, Divinylbenzene (DVB) 80%: Sinopharm Chemical Reagent Co., Ltd.; Matrine 99%: Tianjin Fuyu Fine Chemical Co., Ltd.; α-methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), 4,4'-azobis(4-cyanovaleric acid) (ACPA), 2,2'azobisisobutyronitrile (AIBN): Analytical grade, Tianjin Damao Chemical Reagent Factory.

[0043] VECTOR-33FT-IR Fourier Transform Infrared Spectrometer: Bruker, Germany; MASTERSIZER 2000 Laser Particle Size Analyzer: Malvern Instruments, UK; HITACHI S-4300 Scanning Electron Microscope: Hitachi, Japan; UV-2550 Ultraviolet Spectrophotometer: Shimadzu, Japan .

[0044] 2. Preparation of surface molecularly imprinted polymer carrier microspheres:

[0045] S01. GMA and DVB in different proportions (20%, 30%, 40%, 50%, 60%) (the molar...

Embodiment 2

[0064] The aforementioned surface MIMs were named as ZK-607 as the adsorbent. The scanning electron microscope image of ZK-607 adsorbent molecularly imprinted polymer is shown in Figure 7 shown. The present embodiment provides a method for extracting and purifying matrine and oxymatrine in Sophora flavescens, comprising the following steps:

[0065] S1. Sophora flavescens powder is ultrasonically extracted with ethanol as an extraction solvent, and after filtration, the filtrate is collected and the solvent is evaporated to obtain a primary extract; the Sophora flavescens powder is obtained by pulverizing the Sophora flavescens and crushing it to a sieve of 20-40. The volume ratio concentration of ethanol used is 60%, and the liquid-material ratio of the ultrasonic extraction is set to 10:1. The ultrasonic power of the ultrasonic extraction was 100 Hz, and the extraction was performed 3 times, and the extraction time of each extraction was 0.5 h. The extraction yield of th...

Embodiment 3

[0074] The aforementioned surface MIMs were named as ZK-607 as the adsorbent. The scanning electron microscope image of ZK-607 adsorbent molecularly imprinted polymer is shown in Figure 7 shown. The present embodiment provides a method for extracting and purifying matrine and oxymatrine in Sophora flavescens, comprising the following steps:

[0075] S1. Sophora flavescens powder is ultrasonically extracted with ethanol as an extraction solvent, and after filtration, the filtrate is collected and the solvent is evaporated to obtain a primary extract; the Sophora flavescens powder is obtained by pulverizing the Sophora flavescens and crushing it to a sieve of 20-40. The volume ratio of ethanol used is 80%, the ultrasonic extraction, the liquid-to-material ratio is set to 10:1, the ultrasonic power of the ultrasonic extraction is 80 Hz, and the extraction is performed 3 times, and each extraction time is 1 h. The extraction yield of the primary extract was 5.1%.

[0076] S2. ...

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Abstract

The invention discloses an extraction and purification method for matrine and oxymatrine in Sophora flavescens. According to the method, ultrasonic extraction, organic solvent extraction and various separation techniques are applied scientifically and comprehensively, organic combined points of the conventional extraction and purification methods are summarized, ultrasonic primary extraction is performed with ethanol with reasonable concentration, then total alkali in the Sophora flavescens is extracted effectively by macroporous resin, and finally, the matrine and the oxymatrine are obtainedby column chromatographic separation. Particularly, the optimal separation effect is obtained by applying specific adsorbent as filler. The extraction process is fine, and the extraction rate and thepurity of the obtained matrine and oxymatrine are 91% or higher. The process is simple, industrialization is easy to realize, the product purity is high, strong guarantee is provided for antiviral, anti-inflammatory and antitumor application of the matrine and the oxymatrine, and the matrine and the oxymatrine have good market development prospect.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of natural active substances in Chinese medicinal materials, and more particularly, the invention relates to a method for extracting and purifying matrine and oxymatrine in Sophora flavescens. Background technique [0002] Sophora flavescens is the dry root of the leguminous plant Sophors flavescens Ait. It is bitter in taste, cold in nature, and has the functions of clearing dampness and killing insects. It is clinically used to treat dysentery, yellow gallbladder and skin itching. In recent years, it has also been found to have pharmacological effects such as anti-tumor and whitening. [0003] Sophora flavescens mainly contains alkaloids such as matrine and oxymatrine. Among them, matrine is a natural active substance with significant health care effects on human health, which has attracted widespread attention from the international community. It has been proved by scientifi...

Claims

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Application Information

Patent Timeline
04 May 2018
Publication
CN107987079A
IPC
C07D471/22
Inventors
程杏安; 蒋旭红