Isatis root pre-treatment method for fermenting extraction of isatis root polysaccharide

A technology of isatis root polysaccharide and isatis root, applied in plant cells and other directions, can solve the problem of low extraction rate of isatidis polysaccharide, and achieve the effects of shortening the proliferation time, reducing mass transfer resistance, and improving the extraction rate of polysaccharide.

Inactive Publication Date: 2018-05-04
芮志行
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Technical problem to be solved by the present invention: Aiming at the problem that the extraction rate of Radix isatidis polysaccharide by microbial fermentation is not high at present, provide a Radix isatidis pretreatment method for fermenting and extracting Radix isatidis polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Plant culture medium: in parts by mass, 60 parts of ammonium nitrate, 100 parts of potassium nitrate, 120 parts of potassium dihydrogen phosphate, 200 parts of magnesium sulfate, 140 parts of calcium chloride, 0.02 parts of 6-BA, 0.02 parts of naphthaleneacetic acid , 20 parts of sucrose, 20 parts of agar, and 400 parts of water to obtain a plant culture medium.

[0031] A method for pretreatment of Radix Radix isatidis for fermenting and extracting Radix Radix Radix polysaccharide, comprising the following steps:

[0032] (1) Take lateral buds with internodes from Radix isatidis plants as explants, soak them in 70% ethanol solution for 30 seconds, then soak them in 0.1% mercuric chloride solution for 5 minutes, take out Radix isatidis was rinsed 3 times with sterile water, inoculated into plant culture medium, cultured at 23°C for 5 days, the light intensity was 2000lx, and the light time was 12h per day. The explants of Radix isatidis after cultivation were added with ...

Embodiment 2

[0037] Plant medium: in parts by mass, 65 parts of ammonium nitrate, 105 parts of potassium nitrate, 125 parts of potassium dihydrogen phosphate, 225 parts of magnesium sulfate, 145 parts of calcium chloride, 0.04 parts of 6-BA, 0.04 parts of naphthaleneacetic acid , 25 parts of sucrose, 23 parts of agar, and 450 parts of water to obtain a plant culture medium.

[0038] A method for pretreatment of Radix Radix isatidis for fermenting and extracting Radix Radix Radix polysaccharide, comprising the following steps:

[0039] (1) Take lateral buds with internodes from Radix isatidis plants as explants, soak them in 70% ethanol solution for 35 seconds, then soak them in 0.1% mercuric chloride solution for 5 minutes, take out Radix isatidis was rinsed with sterile water for 3 times, inoculated into plant medium, cultured at 24°C for 6 days, the light intensity was 2000lx, and the light time was 12h per day. After culturing, Radix isatidis explants were added with activated carbon an...

Embodiment 3

[0044] Plant medium: in parts by mass, 70 parts of ammonium nitrate, 110 parts of potassium nitrate, 130 parts of potassium dihydrogen phosphate, 250 parts of magnesium sulfate, 150 parts of calcium chloride, 0.06 parts of 6-BA, 0.05 parts of naphthaleneacetic acid , 20-30 parts of sucrose, 20-25 parts of agar, and 400-500 parts of water to obtain a plant medium.

[0045] A method for pretreatment of Radix Radix isatidis for fermenting and extracting Radix Radix Radix polysaccharide, comprising the following steps:

[0046] (1) Take lateral buds with internodes from Radix isatidis plants as explants, soak them in 70% ethanol solution for 40 seconds, then soak them in 0.1% mercuric chloride solution for 6 minutes, take out Radix isatidis was rinsed 4 times with sterile water, inoculated into plant culture medium, cultured at 27°C for 7 days, the light intensity was 2000lx, and the light time was 12h per day. After cultivation, Radix isatidis explants were added with activated c...

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Abstract

The invention relates to an extraction method in the technical field of traditional Chinese medicines, in particular to an isatis root pre-treatment method for fermenting extraction of isatis root polysaccharide. The isatis roots are crushed by means of fluid energy milling, so that the isatis root structure can be more fined and extraction of polysaccharide is not affected; by damaging the mechanical supporting wall membrane structures of the isatis roots, polysaccharide can be more fully dissolved in later stage. Steam explosion is carried out for pre-treatment. High-temperature saturated steam quickly permeates into the cell tissues of the isatis roots and component separation and structure change of raw materials are achieved by moment decompression, so that the cell tissues of the isatis roots are damaged; interconnection among fiber bundles is damaged, so that cell walls are broken to form smooth porous structures; the fiber bundles are separated, and the cell walls are broken, so that the functional components wrapped in the fiber bundles or cells are released, the mass transfer ability is obviously reduced, and therefore, a purpose of increasing the polysaccharide extraction ratio is achieved.

Description

technical field [0001] The invention relates to an extraction method in the technical field of traditional Chinese medicines, in particular to a pretreatment method for isatidis root polysaccharides fermented and extracted from isatidis. Background technique [0002] Banlangen, also known as indigo root, blue indigo root, and indigo root, is the dried root of the cruciferous plant Isatis indigo. It has the effects of clearing away heat and detoxifying, cooling blood and relieving sore throat. Freckles, mumps, rotten throat Dansha, big head plague, erysipelas, carbuncle, etc. As a typical representative of antiviral traditional Chinese medicine in my country, Banlangen is the first Chinese medicine research project funded by the National Institutes of Health. [0003] Polysaccharides are a class of biological macromolecules with complex structures, which are composed of multiple monosaccharide molecules connected by glycosidic bonds, and are widely distributed in natural med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12N5/04
CPCC08B37/0003C12N5/04
Inventor 芮志行郑薇林凡
Owner 芮志行
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