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Preparation method of dandelion herb extract

A dandelion extract and dandelion technology are applied in the field of preparation of dandelion extract, can solve the problem of unrecorded extract content and the like, and achieve the effects of good clinical treatment effect, high extraction efficiency and short time consumption

Inactive Publication Date: 2018-05-08
湖南知达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above prior art only provides the preparation method of dandelion extract, and does not record the content of main active ingredients in the extract. The dandelion extract with high content of active ingredients helps to realize the treatment of dandelion for acute bronchitis, urinary tract infection, and anti-tumor. , protect the liver and gallbladder, lower blood sugar and blood fat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the preparation method of dandelion extract

[0043] Contains the following steps:

[0044] 1. Broken dandelion: cut dandelion into small pieces and pulverize to make coarse powder through a 35-mesh sieve;

[0045] 2. Microwave extraction: get dandelion coarse powder, add 75% ethanol solution, set microwave power 550w in microwave extractor, extract with extraction time 55 seconds, obtain extract; Concentrate above extract (concentrate under reduced pressure at 55°C to 1 / 4 volume) to obtain extract;

[0046] 3. Acetone extraction: extract the extract obtained in step 2 with acetone for 3 times to obtain an acetone extract, and obtain the extract after removing the solvent by rotary evaporation;

[0047] 4. Chromatographic column purification: the extract obtained in step 3 is separated and purified by column chromatography, washed with water and then continued to be eluted with ethyl acetate as the eluent, the eluate is collected, and the dandelion extr...

Embodiment 2

[0048] Embodiment 2: the preparation method of dandelion extract

[0049] Contains the following steps:

[0050] 1. Broken dandelion: cut dandelion into small pieces and crush it to make it into a coarse powder passing through a 30-mesh sieve;

[0051] 2. Microwave extraction: get dandelion coarse powder, add 70% ethanol solution, set microwave power 500w in microwave extractor, extract with extraction time 50 seconds, obtain extract; Concentrate above extract (concentrate under reduced pressure at 50°C to 1 / 5 volume) to obtain extract;

[0052] 3. Acetone extraction: the extract obtained in step 2 is extracted 4 times with acetone to obtain an acetone extract, and the extract is obtained after the solvent is removed by rotary evaporation;

[0053] 4. Chromatographic column purification: the extract obtained in step 3 is separated and purified by column chromatography, washed with water and then continued to be eluted with ethyl acetate as the eluent, the eluate is collect...

Embodiment 3

[0054] Embodiment 3: the preparation method of dandelion extract

[0055] Contains the following steps:

[0056] 1. Broken dandelion: cut dandelion into small pieces and crush it to make it into a coarse powder passing through a 40-mesh sieve;

[0057] 2. Microwave extraction: get dandelion coarse powder, add 80% ethanol solution, set microwave power 600w in microwave extractor, extract with extraction time 60 seconds, obtain extract; Concentrate above extract (concentrate under reduced pressure at 60°C to 1 / 3 volume) to obtain extract;

[0058] 3. Acetone extraction: extract the extract obtained in step 2 with acetone for 3 times to obtain an acetone extract, and obtain the extract after removing the solvent by rotary evaporation;

[0059] 4. Chromatographic column purification: the extract obtained in step 3 is separated and purified by column chromatography, washed with water and then continued to be eluted with ethyl acetate as the eluent, the eluate is collected, and ...

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Abstract

The invention relates to a preparation method of dandelion herb extract. The preparation method comprises the following steps: crushing dandelion herb, extracting via microwaves, extracting by using acetone, and purifying by using a chromatographic column. The preparation method comprises the following specific steps: cutting dandelion herb into small sections, crushing the dandelion herb sections, filtering the dandelion herb powder by virtue of a 30-mesh to 40-mesh sieve, and obtaining crude powder; adding 70 to 80 percent ethanol solution into dandelion herb crude powder, extracting for 50to 60 seconds in a microwave extraction instrument with microwave power of 500 to 600 w, and obtaining extract liquid; concentrating the extract liquid to obtain extract; extracting the extract for 3to 4 times by using the acetone, obtaining an acetone extraction solution, removing the solvent in a rotary steaming manner, and obtaining the extract; and separating purifying the extract in a columnchromatographic method, firstly washing, then continuously eluting by using an ethyl acetate elution solution, collecting eluted liquid, drying, and obtaining the dandelion herb extract, wherein thetotal content of luteolin, quercetin, chlorogenic acid and caffeic acid is greater than or equal to 70 percent. The preparation method of the dandelion herb extract is simple in process, easy in industrial production and high in content of active ingredients.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a preparation method of a dandelion extract. Background technique [0002] Dandelion is the dried whole herb of Taraxacum mongolicum Hand-Mazz, Taraxacum borealisinese Kitam or several plants of the same genus. Sweet in taste, slightly bitter, cold in nature, non-toxic, used for treating sores, mastitis, sore throat, damp-heat jaundice, hot stranguria and astringent pain. Dandelion also contains protein, fat, carbohydrates, etc., and has rich nutritional value. Dandelion is often used clinically to treat acute bronchitis and urinary tract infection, and has anti-tumor, liver-protecting and choleretic effects, lowering blood sugar and blood lipids. Because it is rich in minerals, dandelion is also used as a health product to prevent anemia caused by iron deficiency; at the same time, dandelion can whiten the skin, resist free radical damage to the human b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/288G01N30/88
CPCA61K36/288A61K2236/15A61K2236/333A61K2236/35A61K2236/39A61K2236/55G01N30/88
Inventor 赵振伟卞国良李君佩
Owner 湖南知达医药科技有限公司
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