Mucosa-targeted immune enhancer for animals and its application in veterinary vaccines

Abstract

The invention relates to the field of biological medicine, in particular to a mucosa targeted immunopotentiator for animals and an application of the mucosa targeted immunopotentiator for animals to veterinary vaccines. The invention discloses the mucosa targeted immunopotentiator for animals in order to solve the problems in the prior art at present and meet expectation for ideal veterinary vaccines. The mucosa targeted immunopotentiator for animals contains 0.5 mu g-500 mu g / mL CpG, 0.1 mu g-1mg / mL alpha-GC, 0.1 mu g-3 mg / mL Poly(I:C), 1 mu g-5 mg / mL PLGA, 0.1 mu g-5 mg / mL PEI and 1 mu g-10mg / mL chitosan. Meanwhile, the invention further discloses application probability of the mucosa targeted immunopotentiator for animals to the veterinary vaccines and an application method. Therefore,good mucosal immunity can be produced, immunity window period is shortened, antigen dose is effectively reduced, and sterile immunity is produced.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an animal mucosa-targeted immune enhancer and its application in veterinary vaccines. Background technique [0002] All kinds of viral infectious diseases restrict the development of aquaculture industry in my country and the world, cause heavy economic losses, and even endanger human public health security. Inactivated vaccines based on mineral oil adjuvants, which are widely used clinically, have played an important role in the prevention and control of these diseases. However, these vaccines still have certain defects, and the quality of vaccines needs to be further improved. These deficiencies include the following: [0003] First, almost no mucosal antibodies were produced after immunization with inactivated mineral oil-based vaccines. However, the main route of viral infection is the mucosal route. Therefore, the mucous membrane is the first line of defense of the host defens...

AI Technical Summary

Problems solved by technology

[0004] Second, the inactivated vaccine based on mineral oil has relatively large side effects after immunization of the body

Specifically, for broilers that go out quickly, mineral oil vaccines are difficult to absorb, which will affect meat quality and subsequent consumption

For laying hens, it will affect the decline in egg production of laying hens

For pigs, cattle, etc., after immunization with mineral oil vaccine, it will cause severe side effects such as fever, swelling, and decreased feed intake.

[0005] Third, there is a longer period of antibody production after immunization with inactivated mineral oil-based vaccines

After immunization with mineral oil-based vaccines, the qualified antibodies for effective protection are generally achieved in the third week after immunization, resulting in a state of being unable to provide effective protection against field mutant strains for a long time

This brings a great risk of disease to clinical production

[0006] Fourth, mineral oil-based inactivated vaccines require a higher vaccine antigen content

Veterinary vaccines increase the amount of antigen to improve the efficacy of the vaccine after immunization, but veterinary vaccines are limited by low prices, resulting in poor purity of vaccine antigens, more miscellaneous proteins, and greater side effects

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