Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Haemadipsa sylvestris Sylvestin and gene and applications thereof

An antithrombotic peptide and antithrombotic technology, applied in the field of biomedicine, can solve problems such as high bleeding incidence

Active Publication Date: 2018-05-22
云南中科龙津生物科技有限公司
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The target of t-PA is plasminogen, which leads to a higher incidence of bleeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Haemadipsa sylvestris Sylvestin and gene and applications thereof
  • Haemadipsa sylvestris Sylvestin and gene and applications thereof
  • Haemadipsa sylvestris Sylvestin and gene and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The following examples are used to further illustrate the essential content of the present invention, but the content of the present invention is not limited thereto.

[0026] Cloning of the antithrombotic peptide gene of forest mountain leech:

[0027] 1. Extraction of total RNA from forest leech:

[0028] (1) Quickly take out multiple leeches from the liquid nitrogen, cut off the tip of the leeches (including the mouth and pharynx) with scissors, with a total weight of about 100mg, put them in a mortar, add liquid nitrogen and grind them thoroughly. After grinding into powder, add 1ml Trizol extraction buffer (Invitrogen), and grind with liquid nitrogen. After Trizol is dissolved, suck out all the liquid in the mortar into a 1.5ml centrifuge tube, and leave it at room temperature for 5 minutes.

[0029] (2) Add 200 μL of chloroform, shake vigorously for 15 seconds, and place at room temperature for 5 minutes; centrifuge at 12000g for 10 minutes at 4°C; pipette the upper colo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Haemadipsa sylvestris Sylvestin and a gene and applications thereof, and belongs to the technical field of biomedicine. The Haemadipsa sylvestris Sylvestin is a single chainpolypeptide, which is coded by a Haemadipsa sylvestris pain relieving peptide gene. The molecular weight of Haemadipsa sylvestris Sylvestin is 4790.5 Dalton, and the isoelectric point is 6.28. Haemadipsa sylvestris Sylvestin is composed of an amino acid sequence represented by SEQ ID No.1. The gene that encodes Haemadipsa sylvestris Sylvestin is composed of an amino acid sequence represented by SEQ ID No.2. Haemadipsa sylvestris Sylvestin can inhibit FXIIa and kallikrein, can prominently prevent thrombus and acute cerebral hemorrhage, has the advantages of simple structure, convenient artificial synthesis, and strong anti-thrombus activity, and can be used to prepare an FXIIa and kallikrein inhibitor and drugs for preventing thrombus and acute cerebral hemorrhage.

Description

Technical field [0001] The invention belongs to the field of biomedicine technology, and specifically relates to a forest mountain leech (Haemadipsasylvestris) antithrombotic peptide Sylvestin and its gene and application. Background technique [0002] Cerebrovascular disease is one of the main causes of death among middle-aged and elderly people in my country, and it is also one of the key research points of the global health strategy. Among cerebrovascular diseases, the incidence of acute ischemic disease ranks first, with the characteristics of high disability rate, high mortality rate and high recurrence rate. There are more than 1.5 million deaths caused by stroke globally, which is increasing year by year with the increase of the elderly population, seriously threatening human life, health and quality of life. Therefore, research on drugs for the treatment of acute cerebral ischemia has huge social needs and important social significance. [0003] The main cause of acute ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/815C12N15/15A61K38/58A61P7/02A61P9/10
CPCC12N9/6445C12N9/6451C12Y304/21038C07K14/815A61K38/00A61K38/58A61P7/02A61P9/10
Inventor 赖仞刘伟慧龙承波吕秋敏
Owner 云南中科龙津生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com