Control method of antibiotic resistance gene of livestock residue
A technology of breeding waste and drug-resistant genes, which is applied in the field of control of antibiotic-resistant genes of breeding waste, can solve problems such as environmental secondary pollution, and achieve the effects of eliminating potential risks, good environmental benefits and economic benefits
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Embodiment 1
[0024] Example 1: Control of Antibiotic Resistance Genes in Farming Wastes
[0025] Regulate moisture content: firstly, the relative abundance of antibiotics (macrolides and sulfonamides) resistance genes is 3.4×10 -1 About (quantitatively determined by PCR) of livestock and poultry manure, after mixing with biomass waste (wood chips, or bamboo chips, or rice husk) according to the weight ratio of livestock and poultry manure to biomass waste of 1.0:1.5, its moisture content Reduced to 30%.
[0026] Pyrolysis: The livestock and poultry manure mixture after water adjustment is continuously sent into the external thermal pyrolysis furnace by means of screw conveying, and is subjected to pyrolysis treatment in an oxygen-free or oxygen-deficient atmosphere to obtain solid substances. The pyrolysis temperature is 600 ℃, the pyrolysis time is 90min.
[0027] Cooling: cooling the above solid matter in an oxygen-deficient atmosphere to obtain biochar. No antibiotic resistance gene ...
Embodiment 2
[0028] Example 2: Control of Antibiotic Resistance Genes in Aquaculture Wastes
[0029] Regulate the water content: at first will be taken from the livestock and poultry farm, the water content is 50%, the relative abundance of antibiotics (polypeptides, tetracyclines and aminoglycosides) resistance genes is 3.0×10 -3 About (quantitatively determined by PCR) of livestock and poultry manure, mixed with biomass waste (sawdust or rice husk) according to the weight ratio of livestock and poultry manure to biomass waste of 1.0:0.5, and then reduce its moisture content to 20% .
[0030] Pyrolysis: The livestock and poultry manure mixture after adjusting the moisture is continuously sent into the external thermal pyrolysis furnace by means of screw conveying, and is subjected to pyrolysis treatment in an oxygen-free or oxygen-deficient atmosphere to obtain solid substances. The pyrolysis temperature is 700°C, pyrolysis time is 60min.
[0031] Cooling: cooling the above solid matter...
Embodiment 3
[0032] Example 3: Control of antibiotic resistance genes in farming waste
[0033] Regulate water content: at first will be taken from livestock and poultry farm, water content is 90%, antibiotic (quinolones, polypeptides and aminoglycosides) resistance gene abundance is at 1.5 * 10 -5About (quantitatively determined by PCR) of livestock and poultry manure, mixed with biomass waste (wood chips, or bamboo chips, or rice husk, or rice bran) according to the weight ratio of livestock and poultry manure to biomass waste of 1.0:1.25 , and its moisture content is reduced to 35%.
[0034] Pyrolysis: The livestock and poultry manure mixture after moisture adjustment is continuously sent into the external heating pyrolysis furnace by means of screw conveying, and is subjected to pyrolysis treatment in an oxygen-free or oxygen-deficient atmosphere to obtain solid substances. The pyrolysis temperature is 800°C, pyrolysis time is 30min.
[0035] Cooling: cooling the above solid matter i...
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