Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for the determination of free exenatide by competitive chemiluminescence
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of exenatide and chemiluminescence, which is applied in chemiluminescence/bioluminescence, analysis through chemical reaction of materials, biological testing, etc., to achieve the effect of improving sensitivity, increasing sensitivity, and increasing dilution factor
Active Publication Date: 2020-11-27
SHANGHAI MEDICILON INC +1
View PDF4 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
This involves the universality of the method
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0064] 1) Use carbonate buffer (pH9.6) to dilute the mouse anti-exenatide (Exendin-4 1mg / mL) solution to 1 μg / mL, add 100 μL to each well of a 96-well microwell plate, 2- Incubate overnight at 8°C;
[0065] 2) Shake off the liquid in the plate, wash 3 times with PBST (PBS+0.05% Tween-20), 300uL / well, shake for 30sec. Pat dry, add PBS buffer containing 5% skimmed milk, 200uL / well, 37°C, incu...
Embodiment 2
[0075] Standard curve: because the method of the present invention has used competitive reaction mode, so concentration and signal value are in inverse relationship, the proterties of standard curve also present anti-S type, and typical curve sees figure 1 . The standard curves of different analysis batches were verified and compared (see Table 1). The standard curves can be fitted robustly every time, and the differences between different batches are within the range of precision and accuracy requirements, and will not exceed 20% deviation;
[0076]Table 1 Standard curves of different analytical batches
[0077] .
Embodiment 3
[0079] Recovery rate comparison: Add free exenatide to PBS and cell culture medium respectively, the concentration is 8000, 4000, 300, 100, 10, 5pg / mL, measure the recovery rate with reference to the method of Example 1, the recovery rate is In the range of 80% to 120% (see Table 2);
[0080] Table 2 The recovery rate of exenatide in PBS, cell culture medium
[0081] .
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to a method of determining free exenatide according to a competitive chemiluminescence method. The method comprises the steps of coating a microporous plate with an anti-exenatide antibody to form a solid phase antibody, adding exenatide standards of different concentrations and a sample to be determined into pores of the coated microporous plate for incubation for a period of time, adding biotin-labeled exenatide into the pores for continuous incubation for a period of time and washing, adding horse radish peroxidase labeled streptavidin into the pores of the microporousplate for incubation for a period of time and washing, adding chemiluminescence substrates capable of being catalyzed by horse radish peroxidase into the pores of the microporous plate, detecting chemiluminescence signal values after reaction for a period of time, fitting a standard curve with the concentrations of the exenatide standards and the corresponding chemiluminescence signal values, inserting the chemiluminescence signal value of the sample to be determined into the standard curve, and calculating the concentration of the sample to be determined.
Description
technical field [0001] The invention belongs to the fields of biological analysis, pharmaceutical analysis and immunodetection, and in particular relates to a method for measuring free exenatide by a competitive chemiluminescent method. Background technique [0002] Exendin-4 is a GLP-1 analog. Its discovery stems from the study of a poisonous lizard. The animal eats 4 times a year. During fasting, the pancreas shuts down and stops secreting insulin. Substance related. Later studies have shown that the role of Exendin-4 is almost identical to that of GLP-1. Exendin-4 can also act through the GLP-1 receptor, also known as GLP-1 receptor agonist, but the affinity for the receptor It is also stronger than GLP-1. Moreover, the N-terminus of Exendin-4 is not easy to be degraded by DPP-IV, has a longer plasma half-life, has good clinical application value and provides ideas for the treatment of type 2 diabetes. Many pharmaceutical companies have developed Exendin-4-related biot...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More