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Establishment of relative characteristic fingerprints of chemical small molecular components of Dendrobium chinensis

A fingerprint spectrum, the technology of Dendrobium dendrobium, applied in the field of analysis and modeling of fingerprint spectrum, can solve the problems of high instrument requirements, inability to accurately detect macromolecular components, inability to accurately evaluate the quality of Dendrobium dendrobium, etc., to achieve strong pertinence and applicability, The effect of solving the curse of dimensionality

Active Publication Date: 2020-12-25
北京蓝标一成科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, in the existing Dendrobium detection standards, the polysaccharides and other macromolecular medicinal components are detected. However, the detection of polysaccharides and other macromolecular components requires high technology and equipment requirements, and the existing domestic instruments and equipment are usually unable to accurately detect macromolecules. Molecular composition, unable to accurately evaluate the quality of Dendrobium

Method used

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  • Establishment of relative characteristic fingerprints of chemical small molecular components of Dendrobium chinensis
  • Establishment of relative characteristic fingerprints of chemical small molecular components of Dendrobium chinensis
  • Establishment of relative characteristic fingerprints of chemical small molecular components of Dendrobium chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The extraction method of embodiment 1 American flower dendrobium

[0049] Take the dried sample of Dendrobium candidum, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml conical flask, and add 50mL of 75 % Methanol (V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75) was dissolved, and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered with a 0.45 μm microporous membrane to obtain a sample solution of Dendrobium mesicense.

Embodiment 2

[0050] The chromatographic detection method of the Dendrobium officinale extract of embodiment 2

[0051] 1. Extraction and processing method of samples for determination of small molecular components in Dendrobium meihua:

[0052] Take 1.00g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a 100ml volumetric flask, add 50ml of methanol-water (75:25) accurately, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, After filtration, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 5ml of methanol-water (75:25), the supernatant was passed through a 0.45μm microporous membrane, and the subsequent filtrate was obtained.

[0053] 2. Preparation of reference solution

[0054] Accurately weigh 4.10 mg of saffrontoside and 4.08 mg of naringenin respectively, place them in 10 ml volumetric flasks, add 75% methanol to dissolve and dilute, shake well, and use them as stock solutions. Refrigerate at...

Embodiment 3

[0065] Example 3 Establishment of characteristic fingerprints related to Schaftoside in Dendrobium meihua.

[0066] 1. Dendrobium sample solution preparation

[0067] Take the dry sample of Dendrobium, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL of 75% methanol ( V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75), and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered through a 0.45μm microporous membrane to obtain the product. Table 2 is the linear relationship obtained for the reference substance.

[0068] Table 2 The linear relationship table of the reference substance Schaftoside

[0069]

[0070] 2. Establishment method of the relev...

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Abstract

The invention discloses a method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium loddigesii. The method comprises: 1) respectively performing chromatographic detection on samples having a sample size of n, and obtaining test data with chemical small molecule components schaftoside and / or naringenin as reference components; 2) respectively performing fingerprint analysis on the samples to obtain a peak area value of the fingerprints of all chemical components of dendrobium loddigesii; 3) taking the component content value of schaftoside and / or naringenin in chromatographic data as a response variable, taking a peak area value of other components in the fingerprint as an independent variable to establish an analytical model, and establishing a linear regression model of the small molecular components and the fingerprints; 4) characterizing the contribution degree of the independent variable to regression modeling through a variable correlation coefficient index, and establishing the fingerprints of the related characteristics of the chemical small molecule components. According to the method, correlation analysis of the chemical small molecule component content is achieved, and the quality of the dendrobium loddigesii medicinal material can be effectively identified, controlled and evaluated.

Description

technical field [0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to an analysis and modeling method for the fingerprint of Dendrobium candidum. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium officinale, Dendrobium meihua, Dendrobium dendrobii, and Dendrobium drumsticks. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal use. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing effects. Dendrobium is clinically used to treat a variety of diseases, and has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵田邹婷婷
Owner 北京蓝标一成科技有限公司