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Colloidal gold rapid-testing card and kit for detecting vomitoxin and method for detecting vomitoxin

A technology of vomitoxin and colloidal gold, which is used in measuring devices, instruments, scientific instruments, etc., can solve the problems of long detection time, low specificity and accuracy, and poor stability of vomitoxin, and achieves low detection cost, simple operation, High precision and stability

Active Publication Date: 2018-07-03
COFCO GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of long detection time, low sensitivity, specificity and accuracy, high cost and poor stability of DON in the prior art, and provide a fast, high sensitivity, high specificity, high Colloidal gold rapid test card and kit for detecting deoxynivalenol with accuracy and stability and a method for detecting deoxynivalenol

Method used

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  • Colloidal gold rapid-testing card and kit for detecting vomitoxin and method for detecting vomitoxin
  • Colloidal gold rapid-testing card and kit for detecting vomitoxin and method for detecting vomitoxin
  • Colloidal gold rapid-testing card and kit for detecting vomitoxin and method for detecting vomitoxin

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preparation example Construction

[0045] The inventors of the present invention have found in the course of their research that by carrying out redox reactions with a specific proportion of chloroauric acid and sodium citrate under specific conditions, and cooling the reaction product at a specific rate, uniform particle size can be obtained. Colloidal gold particles with a particle size of 10-35nm, preferably 10-30nm. When the colloidal gold particles are used to prepare a colloidal gold quick test card for the detection of mycotoxins, the sensitivity, specificity and precision of the detection can be effectively improved. Therefore, the preparation method of the colloidal gold particle in the colloidal gold solution of the present invention preferably comprises:

[0046] (i) under the condition of stirring, add sodium citrate to the boiling auric acid solution, make the auric acid and sodium citrate contact reaction 5-15min, obtain reaction product;

[0047] (ii) cooling the reaction product obtained in ste...

Embodiment 1

[0064] This embodiment is used to illustrate the preparation method of the colloidal gold quick test card provided by the present invention

[0065] (1) Preparation of colloidal gold solution

[0066] Content is that the chloroauric acid solution of 1.3% by weight is placed on the heating magnetic stirrer, is heated to boiling, adds 2.5ml trisodium citrate solution rapidly (the weight ratio of chloroauric acid and trisodium citrate 1:3.5), solution The color begins to change from light blue, blue, purple, and wine red in sequence. When the solution turns into wine red, continue heating for 5 minutes, then cool down to room temperature at a rate of 15°C / min, and store at 4°C for later use. Through transmission electron microscope inspection, the colloidal gold particles are uniform in size, all between 20-25nm, and form a regular spherical shape;

[0067] (2) Preparation of gold-labeled antibody

[0068] 1) Use boric acid buffer to adjust the pH value of the colloidal gold so...

Embodiment 2

[0084] This embodiment is used to illustrate the preparation method of the colloidal gold quick test card provided by the present invention

[0085] (1) Preparation of colloidal gold solution

[0086] Content is that the chloroauric acid solution of 1.2% by weight is placed on the heating magnetic stirrer, is heated to boiling, adds 2.5ml trisodium citrate solution rapidly (weight ratio 1:3.6 of chloroauric acid and trisodium citrate), solution The color began to change from light blue, blue, purple, and wine red in sequence. When the solution turned into wine red, continue heating for 8 minutes, then cool down to room temperature at a rate of 18 °C / min, and store at 4 °C for later use. Through the transmission electron microscope inspection, the colloidal gold particles are consistent in size, all between 15-20nm, and form a regular spherical shape;

[0087] (2) Preparation of gold-labeled antibody

[0088] 1) Use boric acid buffer to adjust the pH value of the colloidal go...

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Abstract

The invention relates to the biological detection field, discloses a colloidal gold rapid-testing card and kit for detecting vomitoxin and a method for detecting the vomitoxin and particularly discloses the colloidal gold rapid-testing card for detecting the vomitoxin. The colloidal gold rapid-testing card comprises a sample feeding region, a conjugate region, an observation region and a water suction region which are sequentially arrayed on a base plate. The invention further discloses the method for detecting the vomitoxin. The method comprises the steps of carrying out pretreatment on a to-be-tested sample so as to obtain a vomitoxin extract, detecting the vomitoxin extract by virtue of the colloidal gold rapid-testing card, and carrying out result analysis. The invention simultaneouslydiscloses the kit for detecting the vomitoxin. The kit is characterized by comprising the colloidal gold rapid-testing card and a water bottle filled with purified water. According to the colloidal gold rapid-testing card, the detection sensitivity, specificity and precision can be rapidly improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a colloidal gold quick test card for detecting vomitoxin, a method for detecting vomitoxin and a kit for detecting vomitoxin. Background technique [0002] Mycotoxins are metabolites produced by fungi growing in food or feed that are harmful to humans and animals. The earliest record of poisoning by mycotoxins is ergot poisoning in Europe in the 11th century, and the clinical symptoms of this poisoning were described in medieval icon paintings. [0003] Common mycotoxins are aflatoxin (B1), deoxynivalenol, zearalenone, fumonisin B1, T-2 toxin, and ochratoxin A. [0004] Deoxynivalenol (DON), scientific name is deoxynivalenol, is a trichothecene toxin produced by certain Fusarium fungi, and it mainly causes symptoms such as loss of appetite, weight loss and metabolic disorders at low doses , high doses can cause vomiting. DON toxin is the least toxic of more than 200 trichot...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/532
CPCG01N33/532G01N33/558G01N33/577
Inventor 蔡军欧静堃李慧何景傅洋罗荣陈飞
Owner COFCO GROUP
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