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Feed composite multi-microfunctional microbial inoculums and application thereof

A technology for functional bacteria and feeding, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of the use effect of microecological preparations, the reduction of repeatability and stability, the use of single strains, and the infection of various bacteria to achieve the suppression of harmful gases Release, prevent disease, promote growth and development

Active Publication Date: 2018-07-06
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the specific application process of probiotics, due to problems such as diverse sources of bacteria, single strains used, miscellaneous bacteria infection, low number of viable bacteria, and inactivation in gastric juice, the use effect, repeatability and stability of probiotics sex will be greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] 1. According to the following steps to produce multi-micro mixed bacterial liquid

[0021] (1) The polymicrobial mixed bacterial liquid is obtained by mixing the mixed bacterial liquid of lactic acid bacteria, the mixed bacterial liquid of yeast and the mixed bacterial liquid of Bacillus according to the mass ratio of 5:2:1.

[0022] (2) The concentration of lactic acid bacteria in the mixed lactic acid bacteria solution is 1.0×10 10 cfu / ml, containing Enterococcus faecalis (Enterococcus faecalis) CICC 20417, Enterococcus faecium (Enterococcus faecium) CICC 20671, Lactobacillus acidophilus (Lactobacillus acidophilus) CICC 22162, wherein the mass ratio between the lactic acid bacteria is about 3:3:1; the mixed lactic acid bacteria solution is to inoculate the pure lactic acid bacteria into the MRS liquid medium, with a pH value of 6.0-6.2, using a numerically controlled fermenter 32 -Cultivated at 34°C for 12 hours, and obtained by gradually amplifying culture accor...

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PUM

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Abstract

The invention discloses feed composite multi-microfunctional microbial inoculums. The feed composite multi-microfunctional microbial inoculums are obtained by mixing lactic acid bacterium mixed bacterial liquid, saccharomycetes mixed bacterial liquid, bacillus mixed bacterial liquid and a thallus protective agent, wherein the mass ratio of the components is 5:2:1:2. Application of the feed composite multi-microfunctional microbial inoculums in the field of feed is that the feed composite multi-microfunctional microbial inoculums are applied to the conventional feed according to 10 kg per ton of feed. The used bacterial strains are probiotics which are allowed to use through audit of the Department of Agriculture, and can exert special effect in the intestinal track of a pig through long-time production practice; and the probiotics can completely exert the effect through commensalism, breeding, competition or phagocytosis, have high propagation speed of the thallus, have strong competitive advantage, can perform industrialized large-scale production, have high survival rate and high stability after use, and can promote the growth and development of the pigs, prevent diseases, inhibit release of harmful gas and reduce mosquitoes and flies in a colony house.

Description

technical field [0001] The invention relates to a compound multi-microfunctional bacterial agent for production of feed and its application, which belongs to the field of biological feed. Background technique [0002] With the development of animal husbandry and the increase of breeding density, many farmers use antibiotics to prevent and control diseases in the breeding process. But people have gradually realized that long-term use of antibiotics can cause harm to human health through the food chain. Therefore, human beings pay more and more attention to food safety. [0003] The research and application of animal intestinal microbial community has overcome the various disadvantages caused by the long-term use of antibiotics to the breeding industry. A green feed additive with no toxicity, no drug resistance, no residue, and no side effects-microecological preparations has gradually attracted attention. And recognized by the world, has broad application prospects. [000...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/18A23K10/18C12R1/01C12R1/23C12R1/72C12R1/865C12R1/10C12R1/125
CPCA23K10/18C12N1/16C12N1/18C12N1/20
Inventor 李浩赖水明覃智斌刘小成杨伟春张敬学周通
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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