Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of pseudomonic acid a

A technology of pseudomonic acid and Pseudomonas fluorescens, which is applied in the field of preparation of pseudomonic acid A, can solve the problems of high production cost, low purity, and low yield, and achieve low cost, simple fermentation process, The effect of short fermentation time

Active Publication Date: 2021-03-12
ZHEJIANG RUIBANG LAB
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the low yield, low purity and high production cost of pseudomonad acid A fermentation in the prior art, the present invention provides a method that can significantly improve the yield of pseudomonad acid A in the fermentation broth of Pseudomonas fluorescens, The preparation method of pseudomonic acid A with simple production process, low additive cost and relatively short fermentation time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of pseudomonic acid a
  • A kind of preparation method of pseudomonic acid a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The seed liquid is adjusted to a concentration of 1.0 g / L (in terms of dry weight of the thalline) with sterile water, and then inoculated into a 100 L fermenter equipped with a 60 L liquid fermentation medium at a volume ratio of 1.0%. The fermentation temperature is 30°C, the ventilation rate is 1:1vvm, and the agitation is 200rpm. First, ferment until the cell concentration reaches 5g / L, and then add 2.0g / L of a mixture of linoleic acid and leucine at one time (the mass ratio of the two is 1:2) into the fermentation broth, add a mixture of ammonia water and sodium citrate (the mass ratio of the two is 2:1) to control the pH at about 5.6, continue to ferment for 72 hours under the same conditions, end the fermentation, and harvest the fermented product.

[0041] After HPLC analysis, the content of pseudomonic acid A was 3147mg / L.

Embodiment 2

[0043] The seed liquid is adjusted to a concentration of 1.0 g / L (in terms of dry weight of the thalline) with sterile water, and then inoculated into a 100 L fermenter equipped with a 60 L liquid fermentation medium at a volume ratio of 1.0%. The fermentation temperature is 30°C, the ventilation rate is 1:1vvm, and the agitation is 200rpm. First, ferment until the cell concentration reaches 5g / L, and then add 2.0g / L of a mixture of linoleic acid and leucine at one time (the mass ratio of the two is 1.5:1) into the fermentation broth, add a mixture of ammonia water and sodium citrate (the mass ratio of the two is 2:1) to control the pH at about 5.6, continue to ferment for 84 hours under the same conditions, end the fermentation, and harvest the fermented product. After HPLC analysis, the content of pseudomonic acid A was 3249mg / L.

Embodiment 3

[0045] The seed liquid is adjusted to a concentration of 1.0 g / L (in terms of dry weight of the thalline) with sterile water, and then inoculated into a 100 L fermenter equipped with a 60 L liquid fermentation medium at a volume ratio of 1.0%. The fermentation temperature is 30°C, the ventilation rate is 1:1vvm, and the agitation is 200rpm. First, ferment until the cell concentration reaches 5g / L, and then add 2.0g / L of a mixture of linoleic acid and leucine at one time (the mass ratio of the two is 1:1) into the fermentation broth, and add a mixture of ammonia water and sodium citrate (the mass ratio of the two is 2:1) to control the pH at about 5.7, continue to ferment for 90 hours under the same conditions, end the fermentation, and harvest the fermented product. After HPLC analysis, the content of pseudomonic acid A was 3462mg / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of pseudomonic acid A, which comprises the following steps: deep-layer fermentation of Pseudomonas fluorescens strains in a liquid fermentation medium until the cell concentration reaches 5g / L; Add a mixture of linoleic acid and leucine at a concentration of 1.0-3.0g / L, and then add a mixture of ammonia water and sodium citrate to control the pH at 5.5-5.8; continue submerged fermentation to the required concentration of pseudomonic acid A Then stop the fermentation. The microbial production method of the present invention can significantly increase the yield of pseudomonic acid A in the Pseudomonas fluorescens fermentation broth, and the yield is as high as 3462 mg / L, which is higher than the yield reported in existing literature.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a preparation method of pseudomonic acid A. Background technique [0002] Pseudomonic acid A (Pseudomonic acid A), the molecular structure is as (I), the chemical name is 9-{4-[5S(2S,3S-epoxy-5S-hydroxyl-4S-methylhexyl)-3R ,4R-dihydroxy-tetrahydropyran-2S-yl]-3-methylbut-2(E)-enoyloxy}nonanoic acid, produced by fermentation of Pseudomonas sp., is a A novel potent immunosuppressant with antifungal, antiproliferative and antitumor activities. It has strong antibacterial activity against Gram-positive bacteria, such as Staphylococcus aureus and Staphylococcus epidermidis. It also has antibacterial effect on some Gram-negative bacteria, such as Haemophilus influenzae and Neisseria gonorrhoeae. Because pseudomonic acid A shows a unique curative effect on the treatment of methicillin-resistant Staphylococcus aureus (MRSA), it has important clinical application prosp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/16C12R1/39
CPCC12P17/162
Inventor 卜光明
Owner ZHEJIANG RUIBANG LAB