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Biological cleaning agent for treating oily sludge and use method

A cleaning agent and biological technology, applied in sludge treatment, chemical instruments and methods, immiscible solvent sludge treatment, etc., can solve the problem of poor biodegradability of chemical surfactants, high concentration and treatment temperature, secondary pollution, etc. problem, to achieve good sludge cleaning activity, good lipophilic properties, and easy preparation

Active Publication Date: 2018-07-20
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, chemical surfactants are mainly used to clean oily sludge. Although chemical surfactants can clean oily sludge well, they require higher concentrations and processing temperatures, and chemical surfactants have poor biodegradability and have certain toxicity. Easy to cause secondary pollution to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Preparation of single rhamnolipid fermentation liquid

[0021] Weigh separately with electronic balance, 30g glucose, 3g NaNO 3 , 1g KH 2 PO 4 , 2g K 2 HPO 4 ·3H 2 O, 0.4gMgSO 4 ·7H 2 O, 1g of yeast powder; add distilled water and mix well, then dilute to 1L with distilled water; add 30% NaOH to adjust the pH of the medium to 7.0; aliquot and sterilize at 121°C for 30min.

[0022] Select the rhamnolipid product to be Pseudomonas stutzeriRhl (for related records of Pseudomonas stutzeri Rhl, refer to Zhao et al.2015Heterologousproduction of Pseudomonas aeruginosa rhamnolipid under anaerobic conditions for microbial enhanced oil recovery) which is completely single rhamnolipid as A strain of monorhamnolipid.

[0023] After the fermentation medium is cooled to room temperature (20-30° C.), inoculate the seed liquid of the bacterium Rh1 that produces monorhamnolipid of 3% of culture medium volume (number of bacteria is 10 5 per ml), cultured for 5 days...

Embodiment 2

[0026] Embodiment 2: Preparation of monorhamnolipid aqueous solution

[0027] The fermented liquid obtained from the above examples was centrifuged at 10,000 g to remove insoluble matter such as bacteria, and the monorhamnolipid in the fermented liquid was extracted by chloroform / methanol (v / v, 2:1) extraction method.

[0028] Utilize thin-layer chromatography to carry out qualitative analysis to the extracted monorhamnolipid product, get the rhamnolipid product after a small amount of extraction and be dissolved in methylene chloride, prepare the monorhamnolipid sample of 200mg / L, sample Spot on a silica gel plate, develop in a developer of chloroform / methanol / acetic acid=65:15:2 (v / v / v), and develop a color with a developer. Under the phenol-sulfuric acid developer, the product showed yellow color, and its Rf value was calculated. Under the phenol-sulfuric acid developer, the product showed yellow color, and there was only one spot on the thin-layer silica gel plate, and it...

Embodiment 3

[0031] Embodiment 3: application example

[0032] Utilize the biological cleaning agent that embodiment 1 and 2 obtain respectively to clean the oily sludge of an oil field in Northwest China

[0033] Weigh 10g of oily sludge with an electronic balance, pour it into a conical flask, add 100ml of the biological cleaning agent with a concentration of 300mg / L obtained in Example 1 or Example 2, stir and mix well, and under the conditions of 37°C and 180rpm, Oscillate to clean the sludge for 48 hours. After cleaning, oil, water and mud were separated, and the cleaned upper layer oil was recovered. The final oil recovery rate of the biological cleaning agent obtained in Example 1 was 56.8%; the final oil recovery rate of the biological cleaning agent obtained in Example 2 was 59.1%.

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Abstract

The invention belongs to the field of solid waste treatment and particularly relates to a biological cleaning agent for treating oily sludge and a use method. The active component of the biological cleaning agent is mono-rhamnolipid, and the concentration of mono-rhamnolipid in the biological cleaning agent is 0.01wt%-0.2wt%. The cleaning agent for the oily sludge is green, environment-friendly, convenient to prepare, good in cleaning effect and wide in application range. The cleaning method based on the cleaning agent has low consumption and can be used for treating oily sludge produced during petroleum extraction, gathering and transportation, refinement and the like.

Description

technical field [0001] The invention belongs to the field of solid waste treatment, and in particular relates to a biological cleaning agent for treating oily sludge and a use method thereof. Background technique [0002] A large amount of oily solid waste is produced in the process of oil extraction, transportation, refining and oily sewage treatment, which has become an important source of soil oil pollution. About 10 million tons of oily mud sand is produced in the country every year. Oily mud sand has been included in the "National List of Hazardous Wastes" and is the primary pollutant in oil fields. The oil content of the sludge is between 10% and 70%. Although the sludge is a kind of pollutant, cleaning the sludge to recover the crude oil makes the sludge a resource. Compared with other sludge treatment methods, the biological treatment of sludge has the characteristics of no secondary pollution, economical application, etc., and its treatment effect and environmental...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/00
CPCC02F11/002
Inventor 赵峰孙惠春张颖刘畅韩斯琴
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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