Non-target substance rapid detection method for illicit drugs in health food

A health food and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to fully detect prohibited drug ingredients in health food, and achieve the effect of simple pretreatment method, high accuracy, and small matrix effect

CN108303486AInactive Publication Date: 2018-07-20INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2018-07-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a full-scan and high-throughput rapid screening detection method for illicit drugs in a health food. Data-dependent acquisition (IDA-MS) and data-independent acquisition (SWATH-MS) high-resolution mass spectrometry technologies are combined with Chemspider and an ACS compound structure database, illegally added western medicine ingredients in the health food are rapidly screened and analyzed according to the accurate parent ion and fragment molecular weights of primary mass spectrometry and secondary mass spectrometry and isotope peaks and other high-resolution mass spectrometry data information in the absence of a standard substance, and illicit drug ingredients in the health food are qualitatively identified under an unknown target substance condition in order toovercome the non-target substance detection problem of the health food. The method has the advantages of high-throughput analysis of the target substances, small matrix effect, high accuracy, simplicity in pretreatment, fastness, accuracy, small false negative error and small false positive error.
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Description

technical field

[0001] The invention relates to the technical field of detection of unknown risk substances in health food, in particular to a non-target rapid detection method of prohibited drugs in health food. Background technique

[0002] Japan first proposed the concept of health food in the 1980s. At present, countries around the world agree with the following definition of health food by the European Union: If it can be well confirmed that a food can not only provide the usual nutritional effects, but also promote the body’s health in a way If it beneficially affects one or more target functions in the body in a way that reduces the risk of disease, then this food can be considered as a functional food. With the development of modern medical and health technology, the life expectancy of human beings is getting longer and longer, which prompts people to pay more attention to health and diet. Health functional food has gradually come into people's sight. Health food has...

Examples

Embodiment 1

[0034] Example 1: Establishment of LC-Q-TOF-MS high-resolution mass spectrometry rapid screening detection method for target substances of prohibited drugs in health food under standard conditions

[0035] The high-resolution mass spectrometry rapid screening detection method for target substances of prohibited drugs in health food includes the following steps:

[0036] (1) Sample pretreatment

[0037] Using QuEChERS technology combined with ultrasonic-assisted extraction technology for pretreatment extraction of the health food to be tested, the extraction conditions are: add 15 mL of acetonitrile and water solution (volume ratio 7:3), 1.0 g of magnesium sulfate and 0.25 g of health food to 1 mL or 1 g of health food Sodium chloride dispersant, 50mg adsorbent C18 and 50mg PSA purifying agent, vortex for 1min, ultrasonic for 15min, take supernatant after centrifugation and stratification, dilute the supernatant to 25mL, take 1mL solution to pass through organic filter membrane...

Embodiment 2

[0071] Example 2: Application of IDA-MS high-resolution mass spectrometry rapid screening and identification method for prohibited drugs in health food without standard products

[0072] A batch of 25 types of health food with weight-loss function were randomly checked on the market to investigate and analyze whether there was any illegal addition of western medicine in this type of health food.

[0073] (1) Sample pretreatment

[0074] The pretreatment method of 25 kinds of health food samples with weight loss function is carried out according to the general solvent extraction method: firstly grind the sample, accurately weigh 1.000g sample, add 15mL methanol, shake and homogenize for 1min, ultrasonic for 15min, centrifuge for 5min, transfer the supernatant Solution, dilute to 25mL, take 1mL sample solution and pass through 0.45 micron filter membrane to be tested by LC-Q-TOF-MS high-resolution mass spectrometer.

[0075] (2) Data collection, to obtain the IDA-MS mass spectr...

Embodiment 3

[0081] Example 3: Application of SWATH-MS high-resolution mass spectrometry screening and identification method for prohibited drugs in health food under the condition of suspicious target

[0082] Spot checks on the health food with the function of relieving physical fatigue on the market, literature reports show that the health food for relieving physical fatigue on the market may be illegally added with PDE-5 inhibitors, which are mainly used to treat male dysfunction diseases. It has a stimulating effect on the human body for a long time, but long-term use has certain side effects on health, and it cannot be added to health food as a functional ingredient, which seriously violates the national food safety law.

[0083] (1) Sample pretreatment

[0084] Using QuEChERS technology combined with ultrasonic-assisted extraction technology to carry out pretreatment extraction of the health food to be tested, the extraction conditions are: add 15mL of acetonitrile and water solutio...