Umbilical cord mesenchymal stem cells combined with medicine for treating hyperglycemia and diabetic nephropathy

A kind of stem cell and umbilical cord technology, applied in the field of hyperglycemia and diabetic nephropathy drugs, can solve the problems of patients with edema, weight, liver damage, etc., achieve the effect of controlling blood sugar level and improving kidney function

Active Publication Date: 2018-07-24
深圳汉盛再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs are mainly insulin sensitizers, but long-term use of these sensitizers can cause edema and weight gain in patients, and very few patients will also experience severe liver damage

Method used

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  • Umbilical cord mesenchymal stem cells combined with medicine for treating hyperglycemia and diabetic nephropathy
  • Umbilical cord mesenchymal stem cells combined with medicine for treating hyperglycemia and diabetic nephropathy
  • Umbilical cord mesenchymal stem cells combined with medicine for treating hyperglycemia and diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Human umbilical cord mesenchymal stem cell culture and passage method

[0049] After primary culture, huMSCs (i.e. human umbilical cord mesenchymal stem cells) were placed in DMEM / F12 medium containing 10% FBS, 100U / ml penicillin and streptomycin; then, cultured at 37°C, 5% CO2 Routine culture and subculture in the box; the cells obtained from the subculture were digested with 0.25% trypsin, and the stably proliferating cells were collected.

[0050] will be 10 6 The collected human umbilical cord mesenchymal stem cells were suspended in 100ml PBS buffer solution containing 1% bovine serum albumin, and positive antibodies (CD90, CD105, CD73) and negative antibodies (CD45, CD34, CD11b) were detected by flow cytometry , CD19 and HLA-DR) expression. see results figure 1 , showing that the cultured human umbilical cord mesenchymal stem cells meet the standard of stem cells.

Embodiment 2

[0051] Embodiment 2, the preparation of Chinese medicine extract

[0052] Each Chinese medicine extract is prepared according to the following method:

[0053] Take ginseng, salvia miltiorrhiza, and astragalus, slice, filter, dry and grind into powder, pass through a 80-120 mesh sieve to obtain fine powder, and set aside.

[0054] Crush schisandra and Panax notoginseng, put them in a container, immerse in 6-16 times the volume concentration of 50-80% ethanol solution for 1-10 hours, heat and reflux extraction 3 times, each time for 1-6 hours, filter, and combine the filtrates , ethanol was distilled off under reduced pressure and concentrated to a concentrated solution with a relative density of 1.10-1.13 at 60°C for later use.

[0055] Atractylodes macrocephala was decocted and extracted three times with 12 times, 10 times and 8 times the amount of water respectively, each time for 2 hours, the three water extracts were combined, concentrated under reduced pressure to 1g med...

experiment example 3

[0056] Experimental Example 3 Effects of Chinese Medicine Extracts on the Growth of Human Umbilical Cord Mesenchymal Stem Cells and Glomerular Cells

[0057] Select huMSCs (human umbilical cord mesenchymal stem cells) and HMC (glomerular cells) in good growth state to divide 3×10 3 Each well was inoculated in a 96-well plate. After the cells adhered to the wall, different concentrations of Chinese herbal extracts were added for co-cultivation. The control group did not add Chinese herbal extracts, and the zero wells did not contain cells. Only 100 μL of culture solution was added, and each group was parallelized. Make 5 duplicate wells, add 10 μL CCK8 solution to each group after culturing for 4h, 24h, 48h, and 72h, incubate at 37°C for 4h, select 490nm wavelength to measure the absorbance (OD) value of each well. Effective concentration measured according to cck-8 results. The test results are shown in Figure 2 and Figure 3.

[0058] The test results show that the tradition...

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Abstract

The invention provides application of umbilical cord mesenchymal stem cells in preparing medicine for treating hyperglycemia and diabetic nephropathy by combining a traditional Chinese medicine extract. In the application disclosed by the invention, the medicine for treating the hyperglycemia and the diabetic nephropathy is prepared by combining the umbilical cord mesenchymal stem cells having differentiation capacity and having the capacity of treating diabetes with the traditional Chinese medicine extract which is capable of reducing blood glucose and improving the state of kidney and has aninhibition effect on kidney diseases caused by the diabetes, so that the blood glucose can be effectively controlled, and the kidney function can be improved.

Description

technical field [0001] The present invention relates to the field of drugs for hyperglycemia and diabetic nephropathy, in particular to the application of umbilical cord mesenchymal stem cells combined with various Chinese medicinal materials related to diabetes treatment in the preparation of drugs for treating hyperglycemia and diabetic nephropathy. Background technique [0002] According to the World Health Organization (WHO), by 2030, the number of people with diabetes will increase from 285 million in 2010 to 439 million. Diabetes is one of the most serious health hazards to the population in my country, and various acute and chronic complications of diabetes are the main cause of death in diabetic patients. Diabetic nephropathy is one of the most important complications of microvascular disease in diabetic patients, and it is also the main cause of end-stage renal failure. How to effectively prevent and treat diabetic nephropathy is still one of the important issues fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61P3/10A61P13/12A61K36/481A61K36/258A61K36/344A61K36/537A61K36/815A61K36/488A61K36/79A61K36/57A61K36/284A61K36/482A61K36/804A61K36/8969
CPCA61K35/51A61K36/258A61K36/284A61K36/344A61K36/481A61K36/482A61K36/488A61K36/537A61K36/57A61K36/79A61K36/804A61K36/815A61K36/8969A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2300/00
Inventor 郭少娟
Owner 深圳汉盛再生医学科技有限公司
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