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Specific molecular marker lpmi001 for identification of Ophiopogon japonicus in broad-leaved mountain

A molecular marker and broad-leaved mountain technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of unfound, accurate and efficient identification methods of broad-leaved mountain Ophiopogon japonicus, and achieve a simple method Effect

Active Publication Date: 2019-02-26
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of the present invention, although there are research reports on the identification of Ophiopogon japonicus from many aspects such as morphological markers, physiology and biochemistry, cell microsections and RAPD markers, no accurate and efficient identification method for Ophiopogon japonicus broad-leafed species has been found.

Method used

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  • Specific molecular marker lpmi001 for identification of Ophiopogon japonicus in broad-leaved mountain

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Embodiment 1

[0023] 1. Extraction of genomic DNA from samples of common Ophiopogon japonicus, Ophiopogon japonicus, and Ophiopogon japonicus to be tested

[0024] Take 0.3-0.5g of fresh leaves of common Ophiopogon japonicus, Ophiopogon japonicus, and Ophiopogon japonicus broad-leaved samples to be tested, add liquid nitrogen and grind them into powder in a mortar, and then use the Ezup column plant genomic DNA of Shanghai Bioengineering Technology Co., Ltd. The extraction kit extracts the genomic DNA of common Ophiopogon japonicus, Ophiopogon japonicus, and Ophiopogon japonicus broad-leaved samples, and uses 1.5% agarose gel to perform electrophoresis on the obtained DNA to detect the purity, and uses the NanoDropTM of Thermo Fisher Corporation of the United States to A 1000-type ultra-micro-volume ultraviolet spectrophotometer was used to detect the DNA concentration, then diluted to 50 ng / μL, and stored in a 4-degree refrigerator for later use.

[0025] 2. Synthesis of Specific Molecular...

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Abstract

The invention discloses a specific molecular marker LPMI001 for identifying liriope platyphylla Wang et Tang. The nucleotide sequence of an upstream primer PGMI001-F of the molecular marker LPMI001 isas shown in SEQ ID NO. 1; the nucleotide sequence of a downstream primer LPMI001-R of the molecular marker LPMI001 is as shown in SEQ ID NO. 2; the nucleotide sequence of a specific band obtained bythe amplification, in an individual of the liriope platyphylla Wang et Tang, of the marker is as shown in SEQ ID NO. 3. The specific molecular marker LPMI001 can be used for identifying and detectingthe liriope platyphylla Wang et Tang, and can also be used for detecting a radix ophiopogonis medicinal material; further, the market of the radix ophiopogonis medicinal material is regulated; the adulteration behavior is detected; the safety of the radix ophiopogonis medicinal material is ensured, and in addition, the specific molecular marker can be used for detecting the seedlings of liriope platyphylla Wang et Tang, and has great application potential and better commercial value to guarantee the purity of the seedlings and improve production efficiency.

Description

technical field [0001] The invention belongs to the technical field of molecular identification of medicinal plants, and in particular relates to a specific molecular marker LPMI001 for identification of Ophiopogon japonicus. Background technique [0002] Ophiopogon japonicus was first recorded in "Shen Nong's Materia Medica", listed as top grade, has the effects of nourishing yin and promoting body fluid, moistening lung and clearing heart, and is a commonly used medicinal material for nourishing yin. Modern pharmacological research shows that the efficacy of Liedong is similar to that of Ophiopogon japonicus, both have the functions of enhancing immunity, anti-myocardial ischemia and infarction, lowering blood sugar and anti-tumor, etc., but there are certain differences in indications, usage and dosage. The Pharmacopoeia of the People's Republic of China (2015 edition) regards the dried roots of Liriope spicata (Thunb.) Lour.Var.prolifera Y.T.Ma or Liriope muscari (Decne....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895
Inventor 卢江杰刘玉洋孟一君何金宇刘朋丽王慧中
Owner HANGZHOU NORMAL UNIVERSITY