dsDNA immunomagnetic microsphere system, its preparation method and application, and preservation solution

A technology of magnetic microspheres and preservation solution, applied in instruments, coatings, analytical materials, etc., can solve the problems of steric hindrance, unstable preservation of preservation solution, unstable binding of dsDNA, etc.

Active Publication Date: 2021-07-06
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, to provide a dsDNA immunomagnetic microsphere system and its preparation method, to overcome the existing dsDNA antigens combined on the magnetic microspheres by physical adsorption, that is, relying on electrostatic force. The technical problems of undesired phenomena such as dsDNA binding instability and steric hindrance
[0010] Another object of the present invention is to provide a dsDNA immunomagnetic microsphere preservation solution, to solve the technical problem that the dsDNA immunomagnetic microspheres of this application are unstable in the existing preservation solution
[0011] Another object of the present invention is to provide an anti-dsDNA antibody detection reagent, kit and detection system to solve the technical problem that existing related preparations are used to detect anti-dsDNA antibody with low accuracy

Method used

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  • dsDNA immunomagnetic microsphere system, its preparation method and application, and preservation solution
  • dsDNA immunomagnetic microsphere system, its preparation method and application, and preservation solution
  • dsDNA immunomagnetic microsphere system, its preparation method and application, and preservation solution

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preparation example Construction

[0046] Correspondingly, the embodiment of the present invention also provides a method for preparing the above-mentioned dsDNA immunomagnetic microsphere system. The process of the preparation method is as follows figure 2 shown, see also figure 1 , including the following steps:

[0047] S01. Preparation of magnetic microspheres modified with amino polymer groups: react the activated magnetic microspheres 1 with amino polymers, and then treat the reaction product with a blocking agent to obtain amino polymer groups modified The magnetic microsphere 1;

[0048] S02. Reaction of magnetic microspheres modified with amino polymer group 2 with dsDNA antigen: reacting magnetic microsphere 1 modified with amino polymer group 2 with dsDNA antigen 3 in the presence of an activator.

[0049] Specifically, in the above step S01, the activators used for activating the magnetic microspheres 1 include but are not limited to N,N'-diisopropylcarbodiimide (CDC), 1-(3-dimethylaminopropyl ...

Embodiment 11

[0106] This example provides a dsDNA immunomagnetic microsphere system and its preparation method. Among them, the structure of dsDNA immunomagnetic microspheres is as follows: figure 1 Said, it includes nano magnetic microsphere 1, polyethyleneimine group 2 and dsDNA antigen formed nano magnetic microsphere 1-polyethyleneimine group 2-dsDNA antigen 3.

[0107] The preparation method of the dsDNA immunomagnetic microsphere system comprises the following steps:

[0108] S11. Preparation of amino polymer functionalized magnetic microspheres:

[0109] 1) Take a certain amount of magnetic balls, add 0.5mg of DCC per milligram of magnetic balls, then add DMF solution to make the concentration of magnetic balls 20mg / mL, place in a 38°C water bath and shake or mechanically stir for 2 hours ;

[0110] 2) Remove the supernatant from the activated nano-magnetic sphere solution, and then add carbonate buffer solution with pH 9.5 to make the concentration reach 20 mg / mL (calculated as ...

Embodiment 12

[0114] This example provides a dsDNA immunomagnetic microsphere system and its preparation method. Wherein, the structure of the dsDNA immunomagnetic microsphere is the same as that in Example 11.

[0115] The preparation method of the dsDNA immunomagnetic microsphere system comprises the following steps:

[0116] S21. Preparation of amino polymer functionalized magnetic microspheres: refer to step S11 of Example 11, except that the amount of polyethyleneimine is changed to 5 mg.

[0117] S22. dsDNA cross-linked amino polymer functionalized magnetic ball: same as step S12 in Example 11.

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Abstract

The invention discloses a dsDNA immunomagnetic microsphere system, a preparation method thereof, a preservation solution, an anti-dsDNA antibody detection reagent, a kit and a system. The dsDNA immunomagnetic microsphere system of the present invention includes a magnetic microsphere body, an amino-containing polymer group, and a dsDNA antigen, the amino-containing polymer group modifies the magnetic microsphere body, and the dsDNA antigen is polymerized with the amino group. compound group coupling. The preservation solution is used for the preservation of the dsDNA immunomagnetic microsphere system. Anti-dsDNA antibody detection reagents, kits and systems include a dsDNA immunomagnetic microsphere system. The dsDNA immunomagnetic microsphere system structure of the present invention is stable, the coating amount of dsDNA antigen is high, the steric hindrance effect is low, the binding efficiency and binding amount with antibodies are high, and the detection sensitivity of antibodies is improved.

Description

technical field [0001] The invention belongs to the technical field of medical reagents, and in particular relates to an anti-dsDNA antibody detection reagent and a preparation method thereof, an anti-dsDNA antibody detection kit, an anti-dsDNA antibody detection system and their applications. Background technique [0002] Systemic lupus erythematosus (SLE) is a systemic autoimmune disease involving small blood vessels and connective tissues in multiple organs and systems. In Europe, the annual new incidence rate of SLE is about 25 / 100,000, while the incidence rate in North America is even higher. SLE is prone to occur in young women, and relapses and remissions often appear alternately during the course of the disease. Antinuclear antibodies and other autoantibodies against nucleic acids, nucleoproteins, and histones can be produced in patients. These autoantibodies combine with corresponding antigens to form immune complexes, which can be deposited in cardiovascular conne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/52
CPCG01N33/52G01N33/54326G01N33/54346G01N2446/90G01N2800/104
Inventor 饶微刘坤袁锦云刘来壮李婷华卢东林
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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