Convection PCR amplification detection system and convection PCR amplification detection method

Abstract

The invention provides a convective PCR amplification detection system and method. The system includes: a microfluidic chip, including a storage structure, a convection PCR tube, an FTA membrane and a waste liquid receiving structure, the first end of the convection PCR tube communicates with the storage cavity of the storage structure, and the second end of the convection PCR tube communicates with the waste liquid The waste liquid cavity of the receiving structure is communicated, and the FTA membrane is arranged inside the convection PCR tube to filter the solution flowing from the first end of the convection PCR tube to the second end and can make the nucleic acid in the solution adsorb on the surface of the FTA membrane; the flow control module , used to make the solution in the storage chamber enter the convection PCR tube and enter the waste liquid chamber after being filtered by the FTA membrane; the heating module is used to heat the substance in the convection PCR tube; the optical detection module is used for the substance in the convection PCR tube. Fluorescence detection. The invention can improve the nucleic acid analysis efficiency.

Description

technical field [0001] The invention relates to the field of life medical detection and diagnosis, in particular to a convective PCR amplification detection system and a convective PCR amplification detection method. Background technique [0002] In the prior art, the nucleic acid analysis method often includes two steps, wherein, the first step cracks the detection sample, then captures and purifies the nucleic acid template; the second step implements the polymerase chain reaction (Polymerase Chain Reaction, PCR) or other isothermal amplification and detection. Polymerase chain reaction is a molecular biology technique used to amplify specific DNA fragments. Polymerase chain reaction generally requires repeated thermocycling steps of the reaction mixture between two or three temperatures to achieve amplification. As a new type of PCR amplification technology, convective polymerase chain reaction (Convective Polymerase Chain Reaction, CPCCR, hereinafter referred to as con...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a convective PCR amplification detection system and a convective PCR amplification detection method, aiming to solve the problem of low efficiency of traditional nucleic acid analysis systems and methods

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