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Metarhizium and Empedobacter brevis compounded insecticide and application thereof

A brevisbacillus and pesticide technology, applied in pesticides, applications, biocides, etc., can solve the problems of limited use scope and effect, and achieve the effect of improving human health, improving the ecological environment, and reducing the amount of use

Inactive Publication Date: 2018-08-03
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, Metarhizium anisopliae and Stenobacter brevis have their own insecticidal spectrum as two biological agents, and both are relatively narrow, resulting in limited application range and effect.

Method used

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  • Metarhizium and Empedobacter brevis compounded insecticide and application thereof
  • Metarhizium and Empedobacter brevis compounded insecticide and application thereof
  • Metarhizium and Empedobacter brevis compounded insecticide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Compound ratio experiment

[0047] A large number of experimental studies in the present invention show that the insecticide prepared by compounding Metarhizium anisopliae and Stenobacter brevis has better insecticidal effect.

[0048] In order to determine the optimal compound ratio of Metarhizium anisopliae and Stenobacter brevis, different ratios of Metarhizium anisopliae. The indoor virulence test of Stablebacillus brevis complex against Spodoptera litura, the results are shown in Table 3.

[0049] The test agent: brevibacterium 1 × 10 10 Spores / mL; Metarhizium anisopliae (M09) 1×10 7 Spores / mL; CK is water.

[0050] Table 3 Different ratios of Metarhizium anisopliae. Toxicity of Stablebacterium brevis complex to Spodoptera litura

[0051]

[0052] The results in Table 3 show that the mixture of the two agents has good virulence activity against Spodoptera litura, among which Metarhizium anisopliae: Stenobacter brevis (V:V) ratio of 5:5 has the...

Embodiment 2

[0054] Embodiment 2: 10% Metarhizium anisopliae. Stenobacterium brevis emulsion in water

[0055] 1. Prepare 10% Metarhizium anisopliae according to the following weight ratio. Stenobacterium brevis emulsion in water

[0056]

[0057] For the above 10% Metarhizium anisopliae. The Metarhizium anisopliae M09 in the Stenobacterium brevis water emulsion is the high sporopollen of Metarhizium anisopliae obtained after extraction of the conidia obtained by solid fermentation, 2.5×10 10 spores / g.

[0058] 2.10% Metarhizium anisopliae. Indoor Toxicity Determination of Stenobacterium brevis Water Emulsion to Lepidoptera Insects

[0059] For the test agent: 10% Metarhizium anisopliae. Stenobacterium brevis emulsion in water.

[0060] Insects to be tested: Spodoptera litura, camphor moth, camphor moth, cinnamon bilobal moth, green and yellow dead leaf moth, large hook-winged moth, green-fronted moth, pear thorn moth, tea long curl moth, double Line steal moth, Poinciana spodop...

Embodiment 3

[0065] Embodiment 3: 12.5% ​​Metarhizium anisopliae. Stenobacterium brevis powder

[0066] 1. Prepare 12.5% ​​Metarhizium anisopliae in the following weight ratio. Stenobacterium brevis powder

[0067]

[0068] For the above 12.5% ​​Metarhizium anisopliae. Metarhizium anisopliae M09 in Stenobacterium brevis powder is the high sporopollen of Metarhizium anisopliae obtained after extraction of conidia obtained from solid fermentation, 2.5×10 10 spores / g.

[0069] 2.12.5% ​​Metarhizium anisopliae. Indoor Toxicity Determination of Stenobacterium breve Powder to Lepidoptera Insects

[0070] The test agent: 12.5% ​​Metarhizium anisopliae. Stenobacterium brevis powder

[0071] Insects to be tested: Spodoptera litura, camphor moth, camphor moth, cinnamon bilobal moth, green and yellow dead leaf moth, large hook-winged moth, green-fronted moth, pear thorn moth, tea long curl moth, double Line steal moth, Poinciana spodoptera, Guangzhou small petal borer, cotton leaf roller. ...

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Abstract

The invention discloses a Metarhizium and Empedobacter brevis compounded insecticide and an application thereof. The insecticide is prepared from Metarhizium, Empedobacter brevis and insecticide acceptable auxiliaries. The insecticide has obvious synergistic effect, and insecticidal toxicity of single chemical can be improved obviously; meanwhile, the action effect after compounding is equivalentto the effect of a chemical agent. The insecticide is compounded from two single biological insecticidal agents as main agents, has good insecticidal activity for defoliator of forestry, has good environmental compatibility and has great significance for improving ecological environment and increasing human health level when widely applied. A production process is simple, use quantity of a pesticide can be reduced substantially, drug resistance of pests can be delayed, and the insecticide has obvious effect on pollution-free control and continuous integrated control of the pests.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a compound insecticide of Metarhizium anisopliae and Stenobacter brevius and its application. Background technique [0002] Forests can regulate the climate, conserve water sources, maintain water and soil, reduce noise, purify the air, beautify the environment, etc. and have complex structures, high diversity, and strong stability. They have important characteristics of great development potential and ecological security, including ecological public welfare forests, timber forests and Urban ecological landscape construction forest, etc. In recent years, the damage of forestry pests and diseases has been on the rise, and some common pests and diseases have risen to become the main pests and diseases, which have seriously occurred and become popular. The types of major plant diseases and insect pests are constantly increasing, seriously causing disease o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/30A01N25/22A01N63/00A01N63/04A01N43/30A01N37/36A01P7/04
CPCA01N25/22A01N25/30A01N37/36A01N43/30A01N63/00A01N63/30
Inventor 杨华赵丹阳秦长生徐金柱揭育泽邱华龙练涛谢国彪梁文洪刘春燕方天松
Owner GUANGDONG ACAD OF FORESTRY
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