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A composite coating for enriching and purifying c-kit+ heart cells and its preparation method

A composite coating and heart cell technology, applied in biochemical equipment and methods, cell culture support/coating, animal cells, etc., can solve the problems of reducing the therapeutic effect of stem cell regenerative therapy, weak adhesion, and large dosage. Achieve the effects of easy control and implementation, weak adhesion and high product yield

Active Publication Date: 2019-04-05
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the screened cells are in a dispersed state and carry sorted magnetic beads, they have defects in the treatment of myocardial damage: large dosage, weak adhesion and low activity, which greatly reduce the therapeutic effect of stem cell regeneration therapy

Method used

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  • A composite coating for enriching and purifying c-kit+ heart cells and its preparation method
  • A composite coating for enriching and purifying c-kit+ heart cells and its preparation method
  • A composite coating for enriching and purifying c-kit+ heart cells and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a method for enriching and purifying c-kit + The preparation method of the composite coating of heart cell, it comprises the following steps:

[0029] s1) Dissolution: Dissolve poloxamer F68 in water, stir well to obtain a hydrophilic polymer A solution; dissolve gelatin in water, and stir well to obtain a hydrophilic polymer B solution; the temperature during dissolution is 60 ℃; in the process of dissolving poloxamer F68 in water, the mass ratio of poloxamer F68 to water is 0.1%; in the process of dissolving gelatin in water, the mass ratio of gelatin to water is 6%;

[0030] s2) mixing: mixing and stirring the hydrophilic polymer A solution and the hydrophilic polymer B solution to obtain a composite coating solution; the hydrophilic polymer A solution and the hydrophilic polymer B dissolve The mass ratio of liquid is 0.1%;

[0031] s3) Sterilization and incubation: filter the composite coating solution with a pore size of 7 μm, add the fil...

Embodiment 2

[0035] This embodiment provides a method for enriching and purifying c-kit + The preparation method of the composite coating of cardiac cells is similar to Example 1, the difference is:

[0036]s1) Dissolution: the hydrophilic polymer A is loxamer F127, and in the process of dissolving poloxamer F127 in water, the mass ratio of poloxamer F127 to water is 5%; hydrophilic polymer B is fibronectin, In the process of dissolving fibronectin in water, the mass ratio of fibronectin to water is 0.1%;

[0037] s2) mixing: the mass ratio of the hydrophilic polymer A solution to the hydrophilic polymer B solution is 20%;

[0038] In addition, this embodiment also provides a composite coating prepared by the preparation method.

Embodiment 3

[0040] This embodiment provides a method for enriching and purifying c-kit + The preparation method of the composite coating of cardiac cells is similar to Example 1, the difference is:

[0041] s1) Dissolving: hydrophilic polymer A is alginate, and the mass ratio of alginate to water is 7% during the process of dissolving alginate in water; hydrophilic polymer B is polylysine, polylysine In the process of dissolving acid in water, the mass ratio of polylysine to water is 10%;

[0042] s2) mixing: the mass ratio of the hydrophilic polymer A solution to the hydrophilic polymer B solution is 60%;

[0043] In addition, this embodiment also provides a composite coating prepared by the preparation method.

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Abstract

The invention discloses a composite coating used for enriching and purifying c-kit<+> cardiac stem cells and a preparation method of the composite coating, belonging to the field of biological materials. The composite coating provided by the invention comprises a hydrophilic polymer A, a hydrophilic polymer B and water, wherein the hydrophilic polymer A is polyether formed by alginate or poly-oxypropylene and polyoxyethylene, and the hydrophilic polymer B is gelatin, fibronectin or polylysine. For the composite coating provided by the invention, the preparation method is simple, the separationmethod of the c-kit<+> cardiac stem cells can be simplified, and the enriched and purified c-kit<+> cardiac stem cell aggregate can well treat cardiac diseases caused by ischemic injuries.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a method for enriching and purifying c-kit + Composite coatings for cardiac cells and methods for their preparation. Background technique [0002] Myocardial damage caused by ischemic cardiomyopathy is one of the diseases with the highest mortality among heart diseases, and cell regenerative therapy is considered to be one of the most potential therapeutic methods. Inducing the proliferation and differentiation of cardiac stem cells, or stimulating their secretion of growth factors is thought to protect and repair damaged myocardial tissue. Studies have shown that c-kit in the heart + The cells have the potential to protect and repair damaged heart muscle. Currently, c-kit + The separation and purification method of cardiac cells is mainly the immunomagnetic bead screening method, which uses the cell surface antigen c-kit to combine with the corresponding screening magneti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0657C12N5/0662C12N2533/30C12N2533/32C12N2533/52C12N2533/54C12N2535/00
Inventor 陈鲲杨膺黄舒婷谢博文王奕翔汤恺刘昊
Owner GUANGZHOU UNIVERSITY