4 results about "Cardiac Stem Cell" patented technology

By expressing specific factors in cardiac stem cells, it was found that their ADM production ability, MMP1 production ability, and / or HGF production ability could be enhanced. Additionally, it was found that such cardiac stem cells exhibit excellent immune-regulatory and / or anti-inflammatory capabilities. Therefore, a cell population comprising such cardiac stem cells can be provided.

This invention discloses a method and system for assessing the differentiation potential of cardiac stem cells, relating to the field of biometrics. It is used for efficient and accurate prediction of cell differentiation potential. Under hypoxic conditions, continuous dynamic imaging of the mitochondrial network of a single cardiac stem cell is performed using a live-cell workstation. Morphometric algorithms are used to quantify network connectivity and cristae remodeling status, establishing a single-cell resolution mitochondrial dynamic remodeling atlas. This atlas is then spatiotemporally correlated with synchronously acquired cellular energy metabolome data to identify specific phenotypic patterns with high differentiation potential. Based on these patterns, a multidimensional feature training set is constructed, and a differentiation potential prediction model is established using a random forest algorithm to screen key precursor features. Finally, the prediction model is used to score the cells to be evaluated, and functional verification is performed by combining single-cell mitochondrial genome copy number variation spectra, screening for high-differentiation-potential cardiac stem cell subpopulations, providing a precise solution for evaluating stem cell differentiation potential.

This utility model relates to a packaging bag for stem cell injection solution. The packaging bag includes a bag body and an infusion interface. The bag body includes a light-proof area and a transparent window area. The inner layer of the light-proof area is polytetrafluoroethylene (PTFE), and the outer layer is a dark-colored coating or dark-colored high-density polyethylene (HDPE). A sealing strip is provided between the transparent window area and the light-proof area. Compared with existing technologies, this packaging bag is specifically designed for the biochemical characteristics of cardiac stem cells and their injection solution. Using HDPE as the main material, it has good biocompatibility, non-stick properties, and thermal insulation, ensuring both the safety and stability of stem cell storage. The sealing strip between the transparent window area and the light-proof area prevents the injection solution from being exposed to the transparent area and deteriorating before use. A Luer-Lock interface with a double-sealing structure (sealing ring or threaded structure) ensures that the stem cell injection solution is leak-free and uncontaminated during storage and transportation.

The invention discloses a method and a system for evaluating differentiation potential of myocardial stem cells, and relates to the technical field of biological recognition. The method is used for efficiently and accurately predicting the cell differentiation potential. The method comprises the following steps: in a low-oxygen environment, carrying out continuous dynamic imaging on a mitochondrial network of a single myocardial stem cell through a living cell workstation, quantifying a network connectivity and a crest membrane reconstruction state by utilizing a morphological metrology algorithm, and establishing a mitochondrial dynamic reconstruction map of a single cell resolution; performing space-time correlation analysis on the atlas and synchronously acquired cell energy metabolite group data, and identifying a specific phenotypic mode with high differentiation potential; based on the mode, constructing a multi-dimensional feature training set, establishing a differentiation potential prediction model through a random forest algorithm, and screening key precursor features; finally, to-be-evaluated cells are scored through the prediction model, functional verification is conducted in combination with the single-cell mitochondrial genome copy number variation spectrum, myocardial stem cell subgroups with high differentiation potential are screened, and a precise scheme is provided for stem cell differentiation potential evaluation.