Purpose of salvianic acid A sodium for inducing cardiac stem cell proliferation

A technology of cardiac stem cells and danshensu sodium, applied in the field of medicine, can solve the problems of poor stability of danshensu, and achieve the effects of exact curative effect, reducing immune rejection and reducing mortality.

Inactive Publication Date: 2012-10-10
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, Danshensu has poor stability and usually exists as its sodium salt, Danshensu Sodium. Danshensu Sodium and Danshensu have the same pharmacological effects

Method used

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  • Purpose of salvianic acid A sodium for inducing cardiac stem cell proliferation
  • Purpose of salvianic acid A sodium for inducing cardiac stem cell proliferation
  • Purpose of salvianic acid A sodium for inducing cardiac stem cell proliferation

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with embodiment.

[0021] 1. Material method

[0022] 1.1 Reagent Fetal bovine serum (FBS, Hyclone); EDTA (Sigma), IMDM medium (Iscove's Modified Dulbecco's Medium, Gbico), penicilin / streptomycin (Gbico), collagenase (Collagenase, Sigma), Mercaptoethanol (Beijing). Take 0.02g of Collagenase, make up the required concentration with 20mL of D-hank’s solution, and then filter and sterilize it for later use. Febronectin, bFGF, EGF, LIF, HEPES and ITS are all products of BD.

[0023] 1.2 Instruments Milipore water purifier (Academic-120, Millipore, USA); electronic balance (Sartorius, Germany), deep-low temperature refrigerator (Thermo Forma, USA; inverted phase contrast microscope, Olympus, Japan; centrifuge (Eppendorf); electric heating magnetic stirrer (Germany IKA); 96-well culture plate, 6-well culture plate (Nunc, Denmark); micro-adjustable sample injector (Eppendorf Company), electric heating cons...

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Abstract

The invention provides a purpose of salvianic acid A sodium for inducing in vitro cultured cardiac stem cell proliferation. The research finds that the salvianic acid A sodium has the effect for inducing in vitro cultured cardiac stem cell proliferation and provides enough stem cells for clinical treatment. The salvianic acid A sodium can be the salvianic acid A sodium with high purity in the market (the content is greater than 98.5%), and can be prepared by a conventional method. The invention has the advantages that the salvianic acid A sodium-induced cardiac stem cell proliferation can provide enough stem cells for treating myocardial infarction, preventing cardiac arrhythmia after infarction during recovery period and treating the stem cells, the salvianic acid A sodium can reduce theorganism immunity rejection reaction and reduce the mortality of acute myocardial infarction, and the therapeutic effect is definite.

Description

Technical field [0001] The invention belongs to the technical field of medicine, in particular to the use of a traditional Chinese medicine monomer compound danshensu sodium, in particular to the use of danshensu sodium in promoting the proliferation of cardiac stem cells (cardiac stem cells, CSCs) in vitro. Background technique [0002] Cardiovascular disease is the number one killer that endangers human health, and death due to coronary heart disease accounts for about 50% of the total number of deaths. At present, the incidence rate of coronary heart disease accounts for 4%-7% in people over 40 years old, and it is on the rise. Studies have shown that after myocardial infarction, a small number of cardiomyocytes divide and proliferate, but they cannot completely repair myocardial tissue. Therefore, after myocardial infarction, the number of myocardial cells decreases, fibrous scar tissue hyperplasia, ventricular remodeling, cardiac function decline, and eventually develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 孙纪元王四旺程蕊吕勇刚杨倩李岳洋华菲
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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