Purpose of salvianic acid A sodium for inducing cardiac stem cell proliferation
A technology of cardiac stem cells and danshensu sodium, applied in the field of medicine, can solve the problems of poor stability of danshensu, and achieve the effects of exact curative effect, reducing immune rejection and reducing mortality.
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[0020] The present invention will be further described below in conjunction with embodiment.
[0021] 1. Material method
[0022] 1.1 Reagent Fetal bovine serum (FBS, Hyclone); EDTA (Sigma), IMDM medium (Iscove's Modified Dulbecco's Medium, Gbico), penicilin / streptomycin (Gbico), collagenase (Collagenase, Sigma), Mercaptoethanol (Beijing). Take 0.02g of Collagenase, make up the required concentration with 20mL of D-hank’s solution, and then filter and sterilize it for later use. Febronectin, bFGF, EGF, LIF, HEPES and ITS are all products of BD.
[0023] 1.2 Instruments Milipore water purifier (Academic-120, Millipore, USA); electronic balance (Sartorius, Germany), deep-low temperature refrigerator (Thermo Forma, USA; inverted phase contrast microscope, Olympus, Japan; centrifuge (Eppendorf); electric heating magnetic stirrer (Germany IKA); 96-well culture plate, 6-well culture plate (Nunc, Denmark); micro-adjustable sample injector (Eppendorf Company), electric heating cons...
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