Neuregulin and cardiac stem cells

A cardiomyocyte and cell technology, applied in the field of neurangrin and cardiac stem cells, can solve the problems of unclear and inefficient differentiation into cardiomyocytes

Inactive Publication Date: 2011-11-02
ZENSUN (SHANGHAI) SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, differentiation of ES cells into cardiomyocytes in vitro remains an ill-defined, inefficient and relatively non-selective process.

Method used

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  • Neuregulin and cardiac stem cells
  • Neuregulin and cardiac stem cells
  • Neuregulin and cardiac stem cells

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0092] Preparation of neuregren

[0093] The preparation of neuregulin can be according to any obvious related technique. Exemplary techniques for preparing neuregulin can be found in, eg, US Patents 7,226,907 and 5,367,060, WO94 / 026298 and WO03 / 099300, the contents of which are hereby incorporated by reference in their entirety. In certain embodiments, neuregulin is synthesized, for example by solution phase or solid phase peptide synthesis, see Merrifield, 1963, J.Am.Chem.Soc.85:2149; Fields et al, 1990, Int J Pept Protein Res. 35:161-214; Fields et al., 1991, Pept Res. 4:95-101, the contents of which are hereby incorporated by reference in their entirety.

[0094] Neuregulin can be purified by any technique known in the art, such as high performance liquid chromatography, ion exchange chromatography, gel electrophoresis, affinity chromatography and the like. Those skilled in the art are familiar with specific neuregulin purification conditions.

Embodiment 1

[0096] Example 1 The effect of neuregulin on cardiac reconstruction

[0097] experimental method:

[0098] 1. Coronary artery ligation and echocardiography in rats

[0099] 200±20g male Wistar rats (Shanghai Experimental Animal Center, Chinese Academy of Sciences) were intraperitoneally injected with 100mg / kg of Ketamine (drug / body weight), fixed on the rat board in a supine position, and sterilized with progeramine after hair removal on the neck and chest. Make a median incision in the neck, bluntly separate the trachea, place an 18G arteriovenous needle between the 3rd and 5th tracheal cartilages, withdraw the needle core, push the plastic cannula 1-2cm into the trachea, and fix it, ready to be connected to a small animal ventilator (SAR -830 / P ventilator, tidal volume about 1ml / 100g / time, frequency 60 times / minute). An incision was made on the left front of the chest, and blunt dissection was performed to expose the fourth and fifth ribs. The chest wall was penetrated f...

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Abstract

The present invention relates to compositions and methods for inducing cardiomyogenesis in mammalian cells, particularly embryonic stem cells in vitro and in vivo.

Description

technical field [0001] The present invention relates to neuregulin inducing mammalian cell cardiomyocytes in vivo and in vitro, especially inducing embryonic stem cells to generate cardiomyocytes. Background technique [0002] Cardiac (ventricular) hypertrophy is an important adaptive physiological response to increased cardiac operating stress or demands. One of the early cellular changes that occurs after hypertrophic stimuli is mitochondrial synthesis and myofibril expansion (wall thickening) with a proportional increase in individual cell size, but no (or minimal) increase in cell number. [0003] When the ventricles are stressed, the initial response is an increase in sarcomere length. This is followed by an increase in overall muscle mass. When the load is too severe, the contractility of the heart muscle will weaken. In the mildest state, this attenuation manifests itself as a reduction in the velocity of unloaded myocardial contraction or a reduction in the rate o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P9/04
CPCC12N5/00A61K9/00A61K38/1883A61P9/00A61P9/04
Inventor 周明东
Owner ZENSUN (SHANGHAI) SCI & TECH CO LTD
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