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Biologic patch material for cardiac repair

A biological patch and heart technology, applied in the field of biomedical materials, can solve the problems of low mechanical strength, poor biocompatibility, and inability to arbitrarily make pathological parts.

Active Publication Date: 2017-04-05
SHANGHAI EAST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current biomaterials that promote the restoration of cardiac function have many problems, such as poor biocompatibility, cannot be made into the corresponding shape of the pathological site at will, are toxic to cells and tissues, are not conducive to cell growth and differentiation, and are immunogenic. , can not block the invasion and decomposition of inflammatory cells, the mechanical strength is not high, etc.

Method used

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  • Biologic patch material for cardiac repair
  • Biologic patch material for cardiac repair
  • Biologic patch material for cardiac repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] The preparation of embodiment 1 porous structure layer

[0128] Elastin is dissolved in phosphate buffer solution PBS (10mM sodium phosphate, 150mM sodium chloride, pH7.4), forms the solution that concentration is 100mg / ml, adds the mucopolysaccharide (as heparin) that concentration is 10mg / ml in solution ), and finally add a lysine cross-linking agent (such as bis (sulfosuccinimidyl suberate)) to 10mM, and mix quickly. Slowly add the prepared solution to the corresponding mold, transfer the mold to a 37°C incubator and let it stand for 16 hours until it is formed ( figure 1 ), thereby making a porous structure layer consisting of the first structure layer and the second structure layer, and, as Figure 4 As shown, the porosity of the first structural layer is different from that of the second structural layer.

Embodiment 2

[0129] The detection of embodiment 2 biological patch material porosity

[0130] The bio-patch materials were observed by scanning electron microscopy (SEM) and micro-CT scanning in multi-layered sections in the vertical and horizontal directions.

[0131] Such as figure 2 and 3 As shown, the first porosity A1 is 1-10 μm, the second porosity A2 is 20-200 μm, the ratio A2 / A1 of the second porosity A2 to the first porosity A1≥5, preferably ≥8, more preferably Ground, ≥10. And, the thickness of the first structural layer is 0.01-0.05cm, the thickness of the second structural layer is 0.05-0.5cm, preferably, 0.1-0.25cm; the main surface area of ​​the biological patch material is 10-80cm 2 , preferably 25-55cm 2 .

[0132] The results show that the first structural layer has a smaller porosity A1, which can prevent the loss of repair cells, and can also prevent the infiltration of inflammatory cells; the second layer has a larger porosity A2, which can load or accommodate car...

Embodiment 3

[0133] The mechanical performance test result of embodiment 3 biological patch materials

[0134] Use a uniaxial compression-relaxation test (such as Bose ELF3400, etc.) to determine the mechanical strength of the biological patch material. The patches were left in phosphate buffered solution for 5 hours before testing to avoid loss of elasticity due to dehydration of the patches. The entire mechanical strength test was performed in the presence of phosphate buffer solution to keep the patch wet. The test compresses and relaxes the patch with 50% tension at a speed of 30 μm per second, and records the compression value (mm) and load value (Newton) to calculate the final compression coefficient and energy loss rate.

[0135] The calculation method is as follows:

[0136] Compressibility coefficient: Values ​​obtained from linear regression of compression and relaxation curves between 0-0.2 mm / mm.

[0137] Energy loss = 100% X (compression curve area - relaxation curve area) ...

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Abstract

The invention relates to a biologic patch material and concretely relates to a biologic patch material for cardiac repair. The biologic patch material comprises (i) at least two porous structural layers that are compounded together, wherein the porous structural layer comprises a first structural layer for preventing repairing cell loss and a second structural layer for loading (or accommodating) cardiac repairing cells and the repairing cells are selected from cardiac stem cells, cardiac progenitor (precursor) cells or their composition (derived from embryonic stem cells, induced pluripotent stem cells, heart tissue stem cells, bone marrow and peripheral blood), and (ii) repairing cells loaded on the second structural layer, wherein at least a part of or all the repairing cells are located in gaps of the second structural layer. The biologic patch material can load growing of repairing cells and has very good biocompatibility and mechanical strength.

Description

technical field [0001] The invention relates to biomedical materials, in particular to a biological patch material for heart repair. Background technique [0002] With the continuous improvement of the economic level and the deepening of the aging society, the incidence of cardiovascular diseases continues to rise. Heart failure is a clinical syndrome that develops to the end stage of various cardiovascular diseases, and it is also the main cause of death caused by cardiovascular diseases. It is known as the "number one killer" in the cardiovascular field. At present, there are about 117 million heart failure patients in the world, including about 14.621 million in the Americas, 15.586 million in Europe, and 86.364 million in Asia. There are approximately 29.971 million patients with heart failure in my country, accounting for 25.6% of the world total. The resulting medical expenses are increasing year by year, and the social and family burdens are serious. [0003] Altho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/38A61L27/50A61L27/22
Inventor 唐文洁屠一栋刘中民
Owner SHANGHAI EAST HOSPITAL
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